Reduced incidence of insect-bite hypersensitivity in Icelandic horses is associated with a down-regulation of interleukin-4 by interleukin-10 and transforming growth factor-β1

Hamza, Eman; Wagner, Bettina; Jungi, Thomas W.; Mirkovitch, Jelena; Marti, Eliane

doi:10.1016/j.vetimm.2007.10.018

Cited by 32 publications

(25 citation statements)

References 35 publications

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“…This may indicate a trend of more pro-inflammatory and less regulatory cytokines in females. This is still a controversial issue in horses (Hamza et al, 2008;Uner et al, 2013;Wray et al, 2013), but is in line with higher plasma levels of TNF␣ found in mares than in geldings of mixed breeds and ages (Suagee et al, 2013).…”

Section: Sex Is Relevant For Some Immunological Parametersmentioning

confidence: 83%

Influences of age and sex on leukocytes of healthy horses and their ex vivo cytokine release

Schnabel

Steinig

Schuberth

et al. 2015

Veterinary Immunology and Immunopathology

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Section: Sex Is Relevant For Some Immunological Parametersmentioning

confidence: 83%

Influences of age and sex on leukocytes of healthy horses and their ex vivo cytokine release

Schnabel

Steinig

Schuberth

et al. 2015

Veterinary Immunology and Immunopathology

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“…Based on our previous results 50 we also investigated a role for cytokines, in particular IL‐10 and TGF‐β1, in the mechanism of suppression. A pilot experiment had shown increased levels of IL‐10 and TGF‐β1 mRNA in the MLR co‐culture of CD4 + CD25 − with CD4 + CD25 high cells compared to the MLR of CD4 + CD25 − cells as well as freshly isolated (CD4 + CD25 − or CD4 + CD25 high ) cells (data not shown).…”

Section: Resultsmentioning

confidence: 99%

Equine CD4+ CD25high T cells exhibit regulatory activity by close contact and cytokine-dependent mechanisms in vitro

et al. 2011

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Summary Horses are particularly prone to allergic and autoimmune diseases, but little information about equine regulatory T cells (Treg) is currently available. The aim of this study therefore was to investigate the existence of CD4+ Treg cells in horses, determine their suppressive function as well as their mechanism of action. Freshly isolated peripheral blood mononuclear cells (PBMC) from healthy horses were examined for CD4, CD25 and forkhead box P3 (FoxP3) expression. We show that equine FoxP3 is expressed constitutively by a population of CD4+ CD25+ T cells, mainly in the CD4+ CD25high subpopulation. Proliferation of CD4+ CD25− sorted cells stimulated with irradiated allogenic PBMC was significantly suppressed in co‐culture with CD4+ CD25high sorted cells in a dose‐dependent manner. The mechanism of suppression by the CD4+ CD25high cell population is mediated by close contact as well as interleukin (IL)‐10 and transforming growth factor‐β1 (TGF‐β1) and probably other factors. In addition, we studied the in vitro induction of CD4+ Treg and their characteristics compared to those of freshly isolated CD4+ Treg cells. Upon stimulation with a combination of concanavalin A, TGF‐β1 and IL‐2, CD4+ CD25+ T cells which express FoxP3 and have suppressive capability were induced from CD4+ CD25− cells. The induced CD4+ CD25high express higher levels of IL‐10 and TGF‐β1 mRNA compared to the freshly isolated ones. Thus, in horses as in man, the circulating CD4+ CD25high subpopulation contains natural Treg cells and functional Treg can be induced in vitro upon appropriate stimulation. Our study provides the first evidence of the regulatory function of CD4+ CD25+ cells in horses and offers insights into ex vivo manipulation of Treg cells.

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“…7,59,92 No clear correlation was found between the numbers of mast cells at lesion sites and reactivity to intradermal injections of Culicoides extract. 67 Zinc has been shown to have potent immunomodulatory capacity, particularly influencing T-lymphocyte organization and cytokine release. 63,71 Eotaxin and MCP-1 are also upregulated in the skin of horses with insect-bite hypersensitivity.…”

Section: Cause and Pathogenesismentioning

confidence: 99%

Skin Immune System and Allergic Skin Diseases

Scott

2011

Equine Dermatology

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Reduced incidence of insect-bite hypersensitivity in Icelandic horses is associated with a down-regulation of interleukin-4 by interleukin-10 and transforming growth factor-β1

Cited by 32 publications

References 35 publications

Influences of age and sex on leukocytes of healthy horses and their ex vivo cytokine release

Influences of age and sex on leukocytes of healthy horses and their ex vivo cytokine release

Equine CD4+ CD25high T cells exhibit regulatory activity by close contact and cytokine-dependent mechanisms in vitro

Skin Immune System and Allergic Skin Diseases

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