Reduced mammary tumor progression in WAP-TAg/WAP-maspin bitransgenic mice

Zhang, M; Shi, Yihui; Magit, David P.; Furth, Priscilla A.; Sager, Ruth

doi:10.1038/sj.onc.1204006

Cited by 77 publications

(59 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, ETS motifs are frequently located in the vicinity of the transcription start site in a number of promoters that lack a TATA element, including the genes encoding DNA polymerase a and b (Pearson et al, 1991;Yamaguchi et al, 1988), thymidilate synthetase (Jolli and Johnson, 1991), von Willebrand factor (Schwachtgen et al, 1998), Ets2 (Mavrothalassitis et al, 1990, the tumor suppressor gene Maspin (Zhang et al, 1997) and number of myeloid speci®c genes (reviewed by Moreau-Gachelin, 1994). It seems that PARP can be added to this group of genes because it exhibits features typical of TATA-de®cient promoters and has consensus ETS motifs close to the major initiation sites.…”

Section: Inhibition Of Ets1 Expression Enhances Growth Retardation Ofmentioning

confidence: 99%

Regulation of the human poly(ADP-ribose) polymerase promoter by the ETS transcription factor

et al. 1999

View full text Add to dashboard Cite

Ewing's sarcoma (EWS) cells accumulate elevated steady-state levels of poly (ADP-ribose) polymerase (PARP) mRNA and protein. To understand the molecular mechanisms underlying PARP upregulation, we cloned and analysed the 5'-¯anking region of the PARP gene from EWS cells. Nucleotide sequence analysis demonstrated no variations in the PARP promoter region in EWS cells. The PARP promoter encompasses multiple binding motifs for the ETS transcription factor. We have also observed that there is a coordinated up-regulation of the expression of both PARP and ETS1, relative to cells of other human tumor types expressing lower levels of PARP. Transient coexpression of ETS1 in EWS cells resulted in a strong enhancement of PARP-promoter activity. The participation of ETS in the regulation of PARP gene expression was further demonstrated in EWS cells stably transfected with Ets1 antisense cDNA constructs. Antisensemediated down-regulation of endogenous ETS1 resulted in the inhibition of PARP expression in EWS cells, and sensitized these cells to ionizing radiation. These data provide support for ETS regulation of PARP expression levels, and implicate ETS transcription factors in the radiation response of EWS cells.

show abstract

Section: Inhibition Of Ets1 Expression Enhances Growth Retardation Ofmentioning

confidence: 99%

Regulation of the human poly(ADP-ribose) polymerase promoter by the ETS transcription factor

et al. 1999

View full text Add to dashboard Cite

show abstract

“…Accumulative evidence demonstrates that maspin inhibits the progression of prostate and breast tumors at the late stages of invasion and metastasis [1,7,[9][10][11][12]. In several mammary carcinoma cell lines, maspin is undetectable or is expressed at very low levels [1].…”

Section: Introductionmentioning

confidence: 99%

Effects of demethylating agent 5-aza-2′-deoxycytidine and histone deacetylase inhibitor FR901228 on maspin gene expression in oral cancer cell lines

Murakami¹,

Asaumi²,

Maki³

et al. 2004

Oral Oncology

View full text Add to dashboard Cite

Summary Maspin, which belongs to the serine protease inhibitor (serpin) superfamily, has been proposed as a potent tumor suppressor that inhibits cell motility, invasion, angiogenesis, and metastasis. In the present study, we examined the effects of 5-Aza-2'-deoxycytidine (5-Aza-dC), a demethylating agent, and FR901228, a histone deacetylase (HDAC) inhibitor, on maspin expression in oral cancer cell lines. The expression levels of maspin mRNA were divided into two groups, which was the maspin low-expressed and high-expressed cell lines in the 12 oral cancer cell lines. The maspin promoter contained only a few methylated CpG sites in the maspin low-expressed cell lines. Moreover, the methylation status was not altered after 5Aza-dC treatment. However, the transcription of the maspin gene was clearly increased following 5Aza-dC treatment in a number of oral cancer cell lines. These results imply that an action of 5Aza-dC is separate from induction of promoter demethylation. Treatment with FR901228 resulted in a time-dependent stimulation of the re-expression of maspin mRNA as early as 4 hours after treatment in the maspin downregulated cells. The re-expression of the maspin gene may contribute to the recuperation of biological functions linked to FR901228 such as an inhibitory effect on tumor angiogenesis and cell invasion. These results indicate that maspin and its target genes may be excellent leads for future studies on the potential benefits of FR901228, a histone deacetylase inhibitor, in cancer therapy.

show abstract

“…In primary breast cancer cells, maspin is down-regulated and its expression is inhibited in metastasis. It has been suggested that this decrease in maspin expression may be due to the absence of transactivation through the Ets and Ap1 elements in the promoter of maspin (Zhang et al, 1997a). Another report showed that expression of maspin can also be repressed by a negative hormonal response element in the prostate cells (Zhang et al, 1997b).…”

Section: Introductionmentioning

confidence: 99%

High tumoral maspin expression is associated with improved survival of patients with oral squamous cell carcinoma

et al. 2000

View full text Add to dashboard Cite

Maspin, a member of the serpin family of protease inhibitors, is known to have tumor-suppressor functions. However, the association between its expression level and survival has not been demonstrated in human cancer. Using the immunohistochemical technique to examine the expression levels of maspin in 44 cases of oral squamous cell carcinoma (SCC), we found that 66% of the cases expressed low to intermediate levels of maspin and 34% of the cases expressed high levels of maspin. We further examined maspin protein expression in a series of six SCC cell lines from the head and neck, and found that all but one expressed low or no maspin protein. We also compared the clinicopathological features of the oral SCC cases with the maspin expression level, and found that high maspin expression was associated with the absence of lymph node metastasis. More importantly, we showed that higher maspin expression was signi®cantly associated with better rates of overall survival, suggesting that high maspin expression may be a favorable prognostic marker for oral SCC.

show abstract

Reduced mammary tumor progression in WAP-TAg/WAP-maspin bitransgenic mice

Cited by 77 publications

References 22 publications

Regulation of the human poly(ADP-ribose) polymerase promoter by the ETS transcription factor

Regulation of the human poly(ADP-ribose) polymerase promoter by the ETS transcription factor

Effects of demethylating agent 5-aza-2′-deoxycytidine and histone deacetylase inhibitor FR901228 on maspin gene expression in oral cancer cell lines

High tumoral maspin expression is associated with improved survival of patients with oral squamous cell carcinoma

Contact Info

Product

Resources

About