Reduced Na-K pump but increased Na-K-2Cl cotransporter in aorta of streptozotocin-induced diabetic rat

Michea, Luis; Irribarra, Verónica; Goecke, I. Annelise; Marusic, Elisa T.

doi:10.1152/ajpheart.2001.280.2.h851

Cited by 24 publications

(19 citation statements)

References 51 publications

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“…Previously published studies documented that in acute STZ-induced diabetes lasting 7-10 days the reduction represented 10-22% in male rats (Davel et al 2000;Javorková et al 2009). Four-six weeks after the STZ treatment, body weight was significantly reduced by 34% (Michea et al 2001) and after 9 weeks the reduction yielded 30% (Ziegelhöffer et al 1996). Prolongation of STZ-induced diabetes to 16 weeks was followed by 56% decrease in body weight of males (Vrbjar et al 2004).…”

Section: Discussionmentioning

confidence: 95%

Influence of sub-chronic diabetes mellitus on functional properties of renal Na+,K+-ATPase in both genders of rats

Javorková¹,

Mezesova²,

Vlkovičová³

et al. 2010

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Abstract. For characterization of Na + ,K + -ATPase, a key enzyme involved in maintenance of intracellular sodium homeostasis, expression of α1 subunit and the ATP-and Na + -binding properties were investigated by Western blot analysis and by enzyme kinetics, respectively. Previous studies documented time-dependent alteration of properties of renal Na + ,K + -ATPase from its mobilization after 8 days to serious deteriorations after 16 weeks of diabetes in rats. Characterizing the critical period during development of the disease, when mobilization of Na + ,K + -ATPase observed in the acute phase turns to its damage, we examined the enzyme properties after 8 weeks lasting diabetes which was induced by a single intraperitoneal administration of streptozotocin in a dose of 65 mg·kg -1 . The unchanged expression of Na + ,K + -ATPase α1-subunit in both genders indicates that 8 weeks represent the time when the mobilization of enzyme synthesis observed previously in acute diabetes is lost. In this time the renal Na + ,K + -ATPase undergoes structural changes in the vicinity of Na + -binding site resulting in worsened affinity to sodium in both genders as indicated by 13% and 18% increase of K Na value in female and male rats, respectively. However, gender specific was the diabetes-induced decrease in affinity to ATP by 18% which occurred in female rats only.

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Section: Discussionmentioning

confidence: 95%

Influence of sub-chronic diabetes mellitus on functional properties of renal Na+,K+-ATPase in both genders of rats

Javorková¹,

Mezesova²,

Vlkovičová³

et al. 2010

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“…Ion imbalance and transporter dysfunction are common events in diabetes. Changes in either the expression or activity of a number of ion transporters have been reported in the diabetic kidney (1), cardiac muscle (20), cardiovasculature (13), and brain (3). There is also evidence of perturbations in the transport of various ions across the BBB in STZinduced diabetes; Na ϩ and K ϩ uptake in the rat BBB decreases, while Cl Ϫ and Ca 2ϩ transport are not altered (2,21).…”

Section: Discussionmentioning

confidence: 99%

“…Expression of these three ion transporters during diabetes has been shown to vary in a tissue-specific manner. Na ϩ -K ϩ -ATPase ␣1-subunit expression is reduced in the aorta of STZ-induced diabetic rats without changes in Na ϩ -K ϩ -ATPase ␣1-subunit in soleus muscle (13). In general, this transporter has reduced activity in diabetic models (13,16,17 the Na ϩ -K ϩ -2Cl Ϫ cotransporter was upregulated in the aorta with no change in expression in the heart (13).…”

Section: Discussionmentioning

confidence: 99%

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Differential Effects of Diabetes on Rat Choroid Plexus Ion Transporter Expression

Egleton

Campos²,

Huber³

et al. 2003

Diabetes

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Though diabetes is a disease with vascular complications, little is known about its effects on the blood-brain barrier or the blood-cerebrospinal fluid barrier (BCSFB). The BCSFB is situated at choroid plexuses located in the lateral, third, and fourth ventricles. Choroid plexuses are the primary site of cerebrospinal fluid (CSF) production and express numerous ion transporters. Previous studies have shown a perturbation of ion transport in the periphery and brain during diabetes. In this study, we investigated the effect of diabetes on ion transporters in the choroid plexuses of streptozotocin (STZ)-induced diabetic rats. Diabetes was induced in male Sprague-Dawley rats by intraperitoneal injection of STZ (60 mg/kg in citrate buffer, confirmed by glucose analysis: 601 ؎ 22 mg/dl diabetic rats, 181 ؎ 46 mg/dl age-matched controls); and at 28 days, rats were killed, choroid plexuses harvested, and protein extracted. Western blot analyses were carried out using antibodies for ion transporters, including Na ؉ -K ؉ -2Cl ؊ cotransporter and the Na iabetes is a disease with numerous vascular complications. Previous studies have shown perturbations in ion transport in both the periphery and the brain during diabetes (1-3). Though diabetes is a vascular disease, little is known regarding the effects of diabetes on the blood-brain barrier (BBB) or the blood-cerebrospinal fluid barrier (BCSFB). It has been reported that diabetes is a risk factor not only for stroke (4,5), but also for normal-pressure hydrocephalus (6,7). Hydrocephalus is caused by excessive retention or production of cerebrospinal fluid (CSF) within the central nervous system. CSF is produced by the choroidal epithelial cells of the choroid plexus (8). The choroid plexuses are also the site of the BCSFB and are located in the lateral, third, and fourth ventricles. Capillaries of the choroid plexus are fenestrated, allowing extracellular fluid formation (unlike other brain capillary beds). CSF production involves the combination of a number of transport systems in the choroid plexus (9) (Fig. 1).Animal models of diabetes have shown that the Na (13), and Na ϩ -K ϩ -ATPase (14 -17) have altered expression and activity in a number of vascular beds. These three transporters are critical for CSF production and pH maintenance (9). In this study, we have investigated the effects of the streptozotocin (STZ) model of type 1 diabetes on expression of the Na ϩ -H ϩ exchanger, NaϪ cotransporter, and Na ϩ -K ϩ -ATPase in the rat choroid plexus. RESEARCH DESIGN AND METHODS Induction of diabetes.All protocols used in this study were approved by the University of Arizona Institutional Animal Care and Use Committee and abide by National Institutes of Health guidelines. Diabetes was induced in male 300-to 325-g Sprague-Dawley rats (Harlan, Indianapolis, IN) via an intraperitoneal injection of 60 mg/kg STZ (Sigma, St. Louis, MO) in sterile phosphate buffered saline. Control animals were injected intraperitoneally with phosphate buffered saline. The animals were housed under ...

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“…reported that a diminished expression of α1 subunit does not affect Na + /K + -ATPase activity, whereas the reduction of α2 protein accounted for the reduction of total Na + /K + -ATPase activity of diabetic animals [49]. Recently, Correll et al show by overexpressing α1 and α2 tg mice, that only overexpression of α2 subunits of the Na + /K + -ATPase reduced cardiac hypertrophy and remodeling [50].…”

Section: Dzurba Et Al Reported That Pretreatment Of Ovariectomized Fmentioning

confidence: 99%

Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high-fat diet

et al. 2017

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Reduced Na-K pump but increased Na-K-2Cl cotransporter in aorta of streptozotocin-induced diabetic rat

Cited by 24 publications

References 51 publications

Influence of sub-chronic diabetes mellitus on functional properties of renal Na+,K+-ATPase in both genders of rats

Influence of sub-chronic diabetes mellitus on functional properties of renal Na+,K+-ATPase in both genders of rats

Differential Effects of Diabetes on Rat Choroid Plexus Ion Transporter Expression

Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high-fat diet

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