Reduced neutrophil superoxide generation and plasma lipoperoxide levels in pigs fed with cod liver oil

Abstract

Circulating neutrophils, isolated from pigs fed for 8 weeks with a diet supplemented with CLO, had an accentuated n-3 fatty acid incorporation into the plasma membrane, as evidenced by an approximately four-fold greater n-3/n-6 ratio as compared with the control diet group. Moreover, the neutrophils of the CLO fed pigs produced less superoxide when stimulated with PMA or f-MLP, as well as showing a more prolonged latency period before O2(-)-generation. In the plasma of pigs fed with CLO there were higher levels of thiobarbituric reactive material and lipofuscin, while the content of GSSG was similar in both dietary groups. The results of this study indicate that dietary supplementation with CLO reduces the activation of circulating neutrophils and favours the presence in the plasma of lipoperoxides.

Hepatoprotective effects of cod liver oil against sodium nitrite toxicity in rats

“…Of note, it has been reported previously that cod liver oil reduced oxidative stress and augmented the antioxidant activity in other animal models that involve oxidative stress like streptozotocininduced diabetes in rats (Hunkar et al, 2002) and daunomycin-induced nephropathy in mice (Ohtake et al, 2002). Moreover, dietary supplementation with cod liver oil inactivates circulating neutrophils and plasma lipoperoxides (Guarnieri et al, 1991). These antioxidant effects of cod liver oil can be attributed to its omega-3 fatty acids and vitamin A contents.…”

Fish oil and vitamin E supplementation in oxidative stress at rest and after physical exercise

“…In long-lasting endurance events, this immune response may be an important secondary source of ROS (5,19). Despite unfavorable effects at rest on lipid peroxidation (11,24,28), FO supplementation decreases the release of proinflammatory cytokines such as interleukin-1, interleukin-6, and tumor necrosis factor-a (13); attenuates neutrophil function, leukotriene chemotactic activity (23), and neutrophil superoxide generation (17); and inhibits arachidonic acid metabolism (23), cyclooxygenase activity, and prostaglandin synthesis (29). All the abovementioned mechanisms can directly or indirectly decrease ROS formation, perhaps more so in response to exhaustive exercise (17).…”