Reduced P‐selectin expression on circulating platelets after prolonged cold preservation in renal transplantation

Abstract

The uremic state in patients with terminal renal insufficiency is accompanied by a bleeding tendency connected with platelet dysfunction. Prolonged cold ischemia and inflammatory interactions between leukocytes, platelets and endothelial cells contribute to ischemia-/reperfusion (I/R) injury and may impair long-term graft survival. We evaluated the influence of the duration of cold preservation time on the expression of platelet GPIIb/IIIa and P-selectin and on the formation of leukocyte-platelet complexes after kidney transplantation. Fourteen patients undergoing kidney transplantation were divided into group I with long preservation time (26.6 +/- 1.9 h) and group II with short preservation time (8 +/- 6.1 h). Five venous blood samples (3 ml) were taken before induction of anesthesia, 12 h, 2, 7 and 14 d after transplantation. Surface expression of the GPIIb/IIIa, P-selectin and the percentage of platelet-granulocyte complexes were quantified by flow cytometry. Additionally blood from seven healthy volunteers was analyzed. GPIIb/IIIa and P-selectin expression on circulating platelets were significantly decreased in the long and the short-term graft preservation group compared with healthy volunteers. A significantly reduced P-selectin expression was found in the long-term preservation group compared with the short-term group. The percentage of platelet-granulocyte complexes also decreased in both preservation groups in the first 2 d after reperfusion and remained in this state in the long-term preservation group. Reduced expression of P-selectin on circulating platelets may be an indicator of I/R injury after prolonged kidney graft preservation.

Platelet Dysfunction in Asphyxiated Newborn Piglets Resuscitated with 21% and 100% Oxygen

“…Whereas the EC 50 of the 100%, but not 21%, reoxygenated group was increased, the negative finding on the maximum aggregatory response (at 10 g/mL collagen) requires cautious interpretation. Nonetheless, this may indicate the reduced number of receptors and responsiveness of the platelets to collagen stimulation (19). Although it has been shown that exposure to hydrogen peroxide in vitro (20) or brief hyperoxia in vivo (21), even in the absence of hypoxia, inhibits both adenosine diphosphate (ADP)-and collagen-induced platelet aggregation, we speculate that the platelet aggregatory dysfunction is secondary to the hypoxia and reoxygenation injury.…”

Protective effect of Salvianolic acid A on ischaemia‐reperfusion acute kidney injury in rats through protecting against peritubular capillary endothelium damages

“…One study showed that rolling platelets along the endothelium occurred after I/R injury (Massberg et al, ). After stimulation, P‐selectin is transported to the platelet surface by fusion of the alpha‐granule membrane with the plasma membrane, which is responsible for the adhesion of activated platelets to endothelium (Scheinichen et al, ). Activated platelets contacting with endothelial cells could aggravate endothelial lesions, which lead to severely renal hypoxia at a microcirculatory level and even plays a central role in the development of subsequent thrombotic microangiopathy.…”

Reduced post‐operative neutrophil activation in liver transplant recipients suffering from post‐hepatitic cirrhosis

“…Flow cytometric samples were prepared as previously described (10). Briefly, leukocytes were identified according to their high‐CD45 expression.…”