1976
DOI: 10.1111/j.1469-8137.1976.tb01487.x
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REDUCED POLLEN VIABILITY IN THE ONION (ALLIUM CEPA)

Abstract: SUMMARYThe fluoreseein test was found to be a rapid and reliable method of assessing pollen viability in onions. This test revealed the presenee of many pollen grains which contained cytoplasm but which were inviable as judged by germination on artificial media. There was very great variation in pollen viability between flowers on a plant, between anthers on a flower and between different parts of a single anther. Both viable and inviable grains oecurred in large clumps consisting chiefly of one type of grain.… Show more

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Cited by 39 publications
(21 citation statements)
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“…According to Ockendon and Gates (1976) there is some difficulty in measuring pollen grain fertility and the available methods tend to overestimate its viability. According to these authors, the acetic-carmine method demonstrates that pollen which does not contain cytoplasm is certainly sterile, while pollen containing cytoplasm is not necessarily fertile.…”
Section: Pollen Grain Viabilitymentioning
confidence: 99%
“…According to Ockendon and Gates (1976) there is some difficulty in measuring pollen grain fertility and the available methods tend to overestimate its viability. According to these authors, the acetic-carmine method demonstrates that pollen which does not contain cytoplasm is certainly sterile, while pollen containing cytoplasm is not necessarily fertile.…”
Section: Pollen Grain Viabilitymentioning
confidence: 99%
“…Segundo Ockendon & Gates (1976), medir a fertilidade do pólen é difícil e os métodos disponíveis tendem a superestimar sua viabilidade. De acordo com estes autores, o método com carmim acético mostra que o pólen sem citoplasma certamente é estéril, mas o que possui citoplasma não é necessariamente fértil.…”
Section: Resultsunclassified
“…Lorenzon & Almeida (1997) e Ockendon & Gates (1976), testando a viabilidade do pólen de cebola com corantes, registraram elevados índices de grãos com conteúdo citoplasmático, entre 84% e 99%. Em contrapartida, percentuais de viabilidade de pólen de cebola foram inferiores quando testados com fluoresceína, que destaca a integridade do plasmalema (Ockendon & Gates 1976, Ali et al 1984. Segundo Ockendon & Gates (1976), este método possibilita uma melhor indicação de viabilidade do pólen do que a simples presença de citoplasma.…”
Section: Resultsunclassified
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