Reduced Sensitivity of <i>Campomanesia adamantium</i> (Cambess.) O. Berg Seeds to Desiccation: Effects of Polyethylene Glycol and Abscisic Acid

Abstract

The Campomanesia adamantium is a threatened species from Brazil Savannah which seeds are desiccation-sensitive and do not withstand storage. This study aimed to reduce the sensitivity of Campomanesia adamantium seeds to desiccation using polyethylene glycol (PEG) and abscisic acid (ABA). Initially, seeds were subjected to PEG (0, −1.48, and −2.04 MPa) with or without ABA (100 μM) during 120 h, followed fast drying (silica gel) or slow drying (laboratory environment), at 20%, 15%, and 10% moisture content. In the second experiment, the seeds were PEG treated (−1.48 MPa) which provided the best results in the first experiment; the seeds were then subjected to different incubation times in PEG (30, 60, 90, or 120 h) and ABA (0, 10 −3 , 10 −4 , and 10 −5 µM), following the seeds were fast dried at 15% moisture content. The slow drying should be avoided, even in seeds previously subjected to osmotic conditioning with or without ABA. Seeds submitted to PEG treatment (−1.48 MPa/120h) without ABA and PEG (−1.48 MPa) with 10 −3 or 10 −4 µM of ABA (90 h), followed by fast drying at 15% moisture content showed reduction of desiccation sensitivity and high germination and vigor when compared to the other treatments.

Embryogenic potential of the callus of gabirobeira, Campomanesia adamantium (Cambess) O. Berg

“…Gabirobeira is propagated sexually, however, the recalcitrance of its seeds hampers this process due to deterioration over time, resulting in a root protrusion rate of less than 50% (Dresch, Scalon, Masetto, & Mussury, 2014). Knowledge about the asexual propagation of this species is still incipient, but holds potential as a viable alternative for its entry into the consumer market and the preservation of the species it its natural state (Dresch, Masetto, Jeromini, & Scalon, 2017;Araújo & Souza, 2018;Oliveira, Silva, Costa Netto, Silva, & Silva, 2011).…”

Biotechnological and socio-environmental potential of Campomanesia adamantium (Myrtaceae): an interdisciplinary review

“…Since seeds of C. adamantium do not withstand storage due to their desiccation sensitivity, difficulties in maintaining them in seed banks and propagating cultivars are an ecologically and financially valid concern. Researchers have found that submitting C. adamantium seeds to a polyethene glycol (PEG) treatment without the increment of abscisic acid (ABA) followed by fast drying (silica gel) up to 15% of water content was able to stimulate protective mechanisms against damage caused by desiccation (Dresch et al, 2017).…”