Reduced Susceptibility to Rifampicin and Resistance to Multiple Antimicrobial Agents among Brucella abortus Isolates from Cattle in Brazil

Pauletti, Rebeca Barbosa; Stynen, Ana Paula Reinato; Mol, Juliana Pinto da Silva; Dorneles, Elaine Maria Seles; Alves, Telma Maria; Souto, Monalisa de Sousa Moura; Minharro, Sílvia; Heinemann, Marcos Bryan; Lage, Andrey Pereira

doi:10.1371/journal.pone.0132532

Cited by 31 publications

(17 citation statements)

References 25 publications

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“…Four isolates of B. melitensis and one isolate of B. abortus showed multidrug resistance against ciprofloxacin, erythromycin, imipenem and rifampicin. These findings are in agreement with the results of Barbosa Pauletti et al who find corresponding resistance among B. abortus isolates from cattle in brazil [62]. All B. melitensis and B. abortus isolates in this study were sensitive to chloramphenicol, gentamicin and tetracycline.…”

Section: Discussionsupporting

confidence: 94%

“…The phenotypic antimicrobial resistance against ciprofloxacin (25%), erythromycin (87.5%), imipenem (25%) and rifampicin (37.5%) of B. abortus isolated in this study was not proved previously. Multidrug resistant strains of B. abortus isolated from cattle in this study were reported previously in Brazil [62]. Four isolates of B. melitensis and one isolate of B. abortus showed multidrug resistance against ciprofloxacin, erythromycin, imipenem and rifampicin.…”

Section: Discussionsupporting

confidence: 71%

“…Only one study from Brazil reported reduced antimicrobial sensitivity in brucellae isolated from cattle [62]. However, the emergence of brucellae isolated from humans phenotypically resistant to ciprofloxacin, gentamycin, streptomycin, rifampicin and trimethoprim-sulfamethoxazole was reported in Egypt, Iran, Qatar, China, Norway and Malaysia [46,48,[63][64][65].…”

Section: Discussionmentioning

confidence: 99%

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Identification, Genotyping and Antimicrobial Susceptibility Testing of Brucella spp. Isolated from Livestock in Egypt

et al. 2019

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Brucellosis is a highly contagious zoonosis worldwide with economic and public health impacts. The aim of the present study was to identify Brucella (B.) spp. isolated from animal populations located in different districts of Egypt and to determine their antimicrobial resistance. In total, 34-suspected Brucella isolates were recovered from lymph nodes, milk, and fetal abomasal contents of infected cattle, buffaloes, sheep, and goats from nine districts in Egypt. The isolates were identified by microbiological methods and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Differentiation and genotyping were confirmed using multiplex PCR for B. abortus, Brucella melitensis, Brucella ovis, and Brucella suis (AMOS) and Bruce-ladder PCR. Antimicrobial susceptibility testing against clinically used antimicrobial agents (chloramphenicol, ciprofloxacin, erythromycin, gentamicin, imipenem, rifampicin, streptomycin, and tetracycline) was performed using E-Test. The antimicrobial resistance-associated genes and mutations in Brucella isolates were confirmed using molecular tools. In total, 29 Brucella isolates (eight B. abortus biovar 1 and 21 B. melitensis biovar 3) were identified and typed. The resistance of B. melitensis to ciprofloxacin, erythromycin, imipenem, rifampicin, and streptomycin were 76.2%, 19.0%, 76.2%, 66.7%, and 4.8%, respectively. Whereas, 25.0%, 87.5%, 25.0%, and 37.5% of B. abortus were resistant to ciprofloxacin, erythromycin, imipenem, and rifampicin, respectively. Mutations in the rpoB gene associated with rifampicin resistance were identified in all phenotypically resistant isolates. Mutations in gyrA and gyrB genes associated with ciprofloxacin resistance were identified in four phenotypically resistant isolates of B. melitensis. This is the first study highlighting the antimicrobial resistance in Brucella isolated from different animal species in Egypt. Mutations detected in genes associated with antimicrobial resistance unravel the molecular mechanisms of resistance in Brucella isolates from Egypt. The mutations in the rpoB gene in phenotypically resistant B. abortus isolates in this study were reported for the first time in Egypt.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 99%

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Identification, Genotyping and Antimicrobial Susceptibility Testing of Brucella spp. Isolated from Livestock in Egypt

et al. 2019

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“…Another study of 27 isolates of Brucella species including Brucella abortus and Brucella Canis in Brazil showed decreased susceptibility to RIF and SXT in 10 and 5 isolates, respectively (24). In addition, a study of 147 B. abortus strains from cattle in Brazil reported reduced susceptibility to RIF in 36% and resistance to RIF in 2% of isolates (25). Reports of such a high prevalence of reduced sensitivity among Brucella isolates from livestock could be a warning alarm for the increasing incidence of RIF-resistant brucellosis in the community.…”

Section: Discussionmentioning

confidence: 98%

Clinical and Diagnostic Aspects of Brucellosis and Antimicrobial Susceptibility of <i>Brucella</i> Isolates in Hamedan, Iran

et al. 2017

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“…It was discussed earlier that in regions with prevalent brucellosis there is a potential of developing of MDR-TB because of a treatment of both diseases with the same frontline antibiotics [ 26 ]. In reality, the provinces of Kazakhstan with high rates of MDR-TB also have high rates of Brucella resistance to rifampricin [ 27 – 29 ]. Among other countries of the world only Kyrgyzstan (which borders Kazakhstan) also has the high rates of both MDR-TB and brucellosis.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial susceptibility of Brucella melitensis in Kazakhstan

Shevtsov

Syzdykov

Kuznetsov

et al. 2017

Antimicrob Resist Infect Control

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BackgroundKazakhstan belongs to countries with a high level of brucellosis among humans and farm animals. Although antibiotic therapy is the main way to treat acute brucellosis in humans there is still little information on a circulation of the antibiotic-resistant Brucella strains in the Central Eurasia. In this article we describe an occurrence of the drug resistance of Brucella melitensis isolates in Kazakhstan which is among the largest countries of the region.MethodsSusceptibilities to tetracyclin, gentamycin, doxycyclin, streptomycin and rifampicin were investigated in 329 clinical isolates of Brucella melitensis using E-test method.ResultsAll isolates were susceptible to streptomycin, tetracycline and doxycycline. 97.3% of the Brucella isolates were susceptible to gentamycin, although only 37.4% of isolates were susceptible to rifampicin. 21.9% of isolates had intermediate resistance, and 26.4% of isolates were resistant to this antibacterial drug.ConclusionIsolates of Brucella melitensis circulating in Kazakhstan are susceptible to streptomycin, doxicyclin, tetracyclin and gentamycin. At the same time the resistance to rifampicin is widespread, almost half of the isolates were rifampicin-resistant (including the intermediate resistance).

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Reduced Susceptibility to Rifampicin and Resistance to Multiple Antimicrobial Agents among Brucella abortus Isolates from Cattle in Brazil

Cited by 31 publications

References 25 publications

Identification, Genotyping and Antimicrobial Susceptibility Testing of Brucella spp. Isolated from Livestock in Egypt

Identification, Genotyping and Antimicrobial Susceptibility Testing of Brucella spp. Isolated from Livestock in Egypt

Clinical and Diagnostic Aspects of Brucellosis and Antimicrobial Susceptibility of <i>Brucella</i> Isolates in Hamedan, Iran

Antimicrobial susceptibility of Brucella melitensis in Kazakhstan

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