Reduced urinary glutamate levels are associated with the frequency of migraine attacks in females

Ragginer, C.; Lechner, Anita; Bernecker, Claudia; Horejsi, Renate; Möller, Reinhard; Wallner-Blazek, M; Weiss, S.; Fazekas, Franz; Schmidt, Ronald; Truschnig-Wilders, M.; Gruber, Hans-Jürgen

doi:10.1111/j.1468-1331.2012.03693.x

Cited by 12 publications

(11 citation statements)

References 30 publications

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“…Elevated urinary glutamic acid levels have been associated with a 4-fold higher risk for migraines in females. 74 Beyond their clinical relevance, the three selected reactions are catalyzed by enzymes with well-known properties, and use a simple, oxygen-dependent reaction that produces H 2 O 2 . 75 The H 2 O 2 is then utilized to oxidize a chromogenic agent in a secondary reaction catalyzed with HRP.…”

Section: Resultsmentioning

confidence: 99%

Modification of microfluidic paper-based devices with silica nanoparticles

Evans

Gabriel

Benavidez

et al. 2014

Analyst

146

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This paper describes a silica nanoparticle-modified microfluidic paper-based analytical device (μPAD) with improved color intensity and uniformity for three different enzymatic reactions with clinical relevance (lactate, glucose, and glutamate). The μPADs were produced on Whatman grade 1 filter paper and using a CO2 laser engraver. Silica nanoparticles modified with 3-aminopropyltriethoxysilane (APTES) were then added to the paper devices to facilitate the adsorption of selected enzymes and prevent the washing away effect that creates color gradients in the colorimetric measurements. Here we show three different enzymatic assays for compounds. According to the results herein described, the addition of silica nanoparticles yielded to significant improvements in color intensity and uniformity. The resulting μPADs allowed for the detection of the three analytes in clinically-relevant concentration ranges with limits of detection (LOD) of 0.63 mM, 0.50 mM, and 0.25 mM for lactate, glucose, and glutamate, respectively. An example of an analytical application has been demonstrated for the semi-quantitative detection of all three analytes in artificial urine. The results demonstrate the potential of silica nanoparticles to avoid the washing away effect and improve the color uniformity and intensity in colorimetric bioassays performed on μPADs.

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Section: Resultsmentioning

confidence: 99%

Modification of microfluidic paper-based devices with silica nanoparticles

Evans

Gabriel

Benavidez

et al. 2014

Analyst

146

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“…The association between the dysregulation of glutamate and migraine attacks is widely described in the literature. Previous studies have observed that migraine patients present higher glutamate levels than controls in plasma, platelets, saliva or cerebrospinal fluid (CSF) (3,18–25), and this effect seems to be reflected in a reduction of urinary glutamate levels (26). It has been reported that in pathologic conditions associated with a large increase of glutamate in brain tissue (which has been shown to occur in pathologies such as ischemia, epilepsy, and presumably migraine), there is a diffusion of glutamate from the extracellular space to the blood following a gradient of concentration, which could explain the increase of systemic glutamate levels observed in these diseases.…”

Section: Discussionmentioning

confidence: 99%

Glutamate oxaloacetate transaminase: A new key in the dysregulation of glutamate in migraine patients

et al. 2013

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“…Disturbances in several amino metabolites have been previously associated with CSD both in brain tissue (Jones et al 2012 ; Cavus et al 2008 ; Levy and Degnan 2013 ; Fabricius et al 1993 ) and body fluids (Ragginer et al 2012 ; de Tommaso et al 2012 ). Our MSI experiments revealed a significant increase in several amino metabolites in the cortex after CSD, notably GABA, an inhibitory neurotransmitter that counters the excitatory glutamate released by CSD.…”

Section: Resultsmentioning

confidence: 99%

Mass spectrometry imaging of amino neurotransmitters: a comparison of derivatization methods and application in mouse brain tissue

et al. 2016

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The detection of small polar compounds such as amino neurotransmitters by MALDI mass spectrometry imaging has been hindered by low-detection sensitivity and background interferences. Recently, several of on-tissue chemical derivatization strategies have been independently reported that enable their detection. Here, we present a comparison between these methods, and demonstrate the visualization of the distributions of up to 23 amino metabolites in tissue. We applied this methodology to detect alterations of these compounds after inducing an experimental cortical spreading depression in mouse brain, which causes profound transient alterations in key neurotransmitters in one hemisphere and is relevant for migraine and various other neurological disorders.Electronic supplementary materialThe online version of this article (doi:10.1007/s11306-015-0926-0) contains supplementary material, which is available to authorized users.

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Reduced urinary glutamate levels are associated with the frequency of migraine attacks in females

Abstract: We show here that female migraineurs exhibit decreased urinary glutamate levels which are associated with a 4.04-fold higher risk for migraine and correlated with patients' frequency of migraine attacks.

Cited by 12 publications

References 30 publications

Modification of microfluidic paper-based devices with silica nanoparticles

Modification of microfluidic paper-based devices with silica nanoparticles

Glutamate oxaloacetate transaminase: A new key in the dysregulation of glutamate in migraine patients

Mass spectrometry imaging of amino neurotransmitters: a comparison of derivatization methods and application in mouse brain tissue

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