Reducing non-productive adsorption of cellulase and enhancing enzymatic hydrolysis of lignocelluloses by noncovalent modification of lignin with lignosulfonate

Lignin-based polyoxyethylene ether enhanced enzymatic hydrolysis of lignocelluloses by dispersing cellulase aggregates

“…The thin lignin film was prepared according to our previous work (Lou et al, 2013b). A 2% (w/w) solution of EHL in 0.75 M ammonium hydroxide was prepared and stored for at least 24 h to ensure complete dissolution.…”

“…Small molecular anionic surfactants restrained the enzymatic hydrolysis (Eriksson et al, 2002), while anionic polymeric surfactants like lignosulfonate, a byproduct from pulping spent liquor, improved the enzymatic hydrolysis (Lou et al, 2013bWang et al, 2013;Zhou et al, 2013). Non-ionic surfactants, especially polyethylene glycol (PEG) based polymer, were found to be the most effective (Eriksson et al, 2002).…”

Associating cooking additives with sodium hydroxide to pretreat bamboo residues for improving the enzymatic saccharification and monosaccharides production

“…Meanwhile, the removal of lignin could reduce the strong surface interaction between the lignin and the enzyme(Lou et al, 2013) Xin et al (2015). also found that alkali pretreatment The enzymatic hydrolysis efficiency (A), correlation between the CS factor and enzymatic efficiency (B), and released monosaccharides (C) of the bamboo residues pretreated by dilute sulfuric acid at different conditions.…”

Effect of the molecular structure of lignin-based polyoxyethylene ether on enzymatic hydrolysis efficiency and kinetics of lignocelluloses

“…Lignosulfonate fractions with low molecular weight (M w ) and high degree of sulfonation could enhance enzymatic hydrolysis of cellulose (Lou et al, 2013b). However, an inhibitory effect on enzymatic hydrolysis was observed using lignosulfonate with large M w and low degree of sulfonation .…”