Simple SummaryDetermining the correct saddle fit is essential in order to optimise the interaction between the horse and rider dyad, and to reduce the risk of back-related problems or loss of performance as a result of incorrect saddle fit. Although there are industry guidelines (Society of Master Saddlers) on correct saddle fit, some saddle fitters (and others) choose to fit saddles that are wider than industry guidelines on the assumption that increased saddle width will enhance equine locomotion and allow the horses’ thoracolumbar spine to function unhindered. This study quantified the effect that a saddle that was one width fitting wider and narrower (based on the Society of Master Saddlers industry guidelines) had on the kinematics of the thoracolumbar spine, thoracolumbar epaxial musculature profiles, equine locomotion, and saddle pressure distribution. It was found that a saddle that was one width fitting wider and narrower affected the kinematics of the thoracolumbar spine, resulting in concavities in epaxial musculature at T13 when using the wide saddle and at T18 when using the narrow saddle. The wide saddle caused areas of high pressures in the cranial region of the saddle and the narrow saddle caused areas of high pressures in the caudal region of the saddle. It is essential that the correct saddle fit is achieved for each horse and rider combination in order to optimise the horse-rider system and reduce the risk of back-related problems or loss of performance that may occur as a result of incorrect saddle fit.AbstractThis study evaluated the effect of saddle tree width on thoracolumbar and limb kinematics, saddle pressure distribution, and thoracolumbar epaxial musculature dimensions. Correctly fitted saddles were fitted by a Society of Master Saddler Qualified Saddle Fitter in fourteen sports horses (mean ± SD age 12 ± 8.77 years, height 1.65 ± 0.94 m), and were altered to one width fitting wider and narrower. Horses were equipped with skin markers, inertial measurement units, and a pressure mat beneath the saddle. Differences in saddle pressure distribution, as well as limb and thoracolumbosacral kinematics between saddle widths were investigated using a general linear model with Bonferroni adjusted alpha (p ≤ 0.05). Compared with the correct saddle width, in trot, in the wide saddle, an 8.5% increase in peak pressures was found in the cranial region of the saddle (p = 0.003), a 14% reduction in thoracolumbar dimensions at T13 (p = 0.02), and a 6% decrease in the T13 range of motion in the mediolateral direction (p = 0.02). In the narrow saddle, a 14% increase in peak pressures was found in the caudal region of the saddle (p = 0.01), an 8% decrease in the range of motion of T13 in the mediolateral direction (p = 0.004), and a 6% decrease in the vertical direction (p = 0.004) of T13. Compared with the correct saddle width, in canter, in the wide saddle, axial rotation decreased by 1% at T5 (p = 0.03) with an 5% increase at T13 (p = 0.04) and a 5% increase at L3 (p = 0.03). Peak pressures increased by...