Reducing risk in the IVF laboratory: implementation of a double witnessing system

Brison, Daniel; Hooper, Michael; Critchlow, JD; Hunter, HR; Arnesen, Rebecca; Lloyd, Alan S.; Horne, G

doi:10.1258/1356262041591131

Cited by 35 publications

(7 citation statements)

References 8 publications

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“…Even though the occurrence of mix-ups is rare, several cases have been reported in fertility clinics around the world (Spriggs, 2003;Bender, 2006). Currently applied measures, such as the labeling of all labware together with the implementation of manual double witnessing (Brison et al, 2004;Magli et al, 2008) or electronic witnessing protocols (Schnauffer et al, 2005;Glew et al, 2006), undoubtedly minimize the risk of sample mismatching due to human error. However, as gametes/embryos are moved from one container to another several times during the course of an ART cycle, the possibility of misidentification still exists.…”

Section: Introductionmentioning

confidence: 99%

Direct embryo tagging and identification system by attachment of biofunctionalized polysilicon barcodes to the zona pellucida of mouse embryos

et al. 2013

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study question: Is the attachment of biofunctionalized polysilicon barcodes to the outer surface of the zona pellucida an effective approach for the direct tagging and identification of cultured embryos?summary answer: The results achieved provide a proof of concept for a direct embryo tagging system using biofunctionalized polysilicon barcodes, which could help to minimize the risk of mismatching errors (mix-ups) in human assisted reproduction technologies.what is known already: Even though the occurrence of mix-ups is rare, several cases have been reported in fertility clinics around the world. Measures to prevent the risk of mix-ups in human assisted reproduction technologies are therefore required. study design, size, duration: Mouse embryos were tagged with 10 barcodes and the effectiveness of the tagging system was tested during fresh in vitro culture (n ¼140) and after embryo cryopreservation (n ¼ 84). Finally, the full-term development of tagged embryos was evaluated (n ¼105).participants/materials, setting, methods: Mouse pronuclear embryos were individually rolled over wheat germ agglutinin-biofunctionalized polysilicon barcodes to distribute them uniformly around the ZONA PELLUCIDA surface. Embryo viability and retention of barcodes were determined during 96 h of culture. The identification of tagged embryos was performed every 24 h in an inverted microscope and without embryo manipulation to simulate an automatic reading procedure. Full-term development of the tagged embryos was assessed after their transfer to pseudo-pregnant females. To test the validity of the embryo tagging system after a cryopreservation process, tagged embryos were frozen at the 2-cell stage using a slow freezing protocol, and followed in culture for 72 h after thawing.main results and the role of chance: Neither the in vitro or in vivo development of tagged embryos was adversely affected. The tagging system also proved effective during an embryo cryopreservation process. Global identification rates higher than 96 and 92% in fresh and frozen-thawed tagged embryos, respectively, were obtained when simulating an automatic barcode reading system, although these rates could be increased to 100% by simply rotating the embryos during the reading process.limitations, reasons for caution: The direct embryo tagging developed here has exclusively been tested in mouse embryos. Its effectiveness in other species, such as the human, is currently being tested.

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Section: Introductionmentioning

confidence: 99%

Direct embryo tagging and identification system by attachment of biofunctionalized polysilicon barcodes to the zona pellucida of mouse embryos

et al. 2013

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“…If a second checker is involved, he or she needs to check patient details independently of the first checker. A robust checking system is a prerequisite in many other areas of our health care systems, such as medication administration 20 and in vitro fertilization checks, 23 not just transfusion practice.…”

Section: Discussionmentioning

confidence: 99%

Blood transfusion administration—one‐ or two‐person checks: which is the safest method?

Watson¹,

Murdock²,

Dorée³

et al. 2008

Transfusion

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“…Sophisticated electronic methods for tracking gametes and embryos are now available from other industries (e.g. RFid (radio frequency identification) tags) and may be required to increase patient confidence, and provide quality assurance, without the significant pitfalls of double witnessing (Brison et al 2004c). The challenge is to implement these advanced technologies while ensuring they are safe.…”

Section: The Challenge Of the New Embryo Technologiesmentioning

confidence: 99%

Challenges imposed by scientific development in ART

Brison

2005

Human Fertility

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The primary goal of ART is to produce a healthy baby which will develop into a healthy adult. There are a number of challenges which must be met in order to achieve this goal, with possibly two foremost at the present time. The first is to eliminate multiple pregnancy by transfer of a single embryo, and the second is to understand the risks involved in ART. In recent years spin-out technologies have also developed from ART, first the diagnosis of genetic disease in the embryo, and now the derivation of embryonic stem cell lines with the potential to treat a range of human health disorders. Current and future scientific developments can help achieve all of these aims, but present a number of challenges to the ART community and regulatory bodies.

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Reducing risk in the IVF laboratory: implementation of a double witnessing system

Cited by 35 publications

References 8 publications

Direct embryo tagging and identification system by attachment of biofunctionalized polysilicon barcodes to the zona pellucida of mouse embryos

Direct embryo tagging and identification system by attachment of biofunctionalized polysilicon barcodes to the zona pellucida of mouse embryos

Blood transfusion administration—one‐ or two‐person checks: which is the safest method?

Challenges imposed by scientific development in ART

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