2023
DOI: 10.3390/ijms241814327
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Reducing Sialylation Enhances Electrotaxis of Corneal Epithelial Cells

Bryan Le,
Kan Zhu,
Chelsea Brown
et al.

Abstract: Corneal wound healing is a complex biological process that integrates a host of different signals to coordinate cell behavior. Upon wounding, there is the generation of an endogenous wound electric field that serves as a powerful cue to guide cell migration. Concurrently, the corneal epithelium reduces sialylated glycoforms, suggesting that sialylation plays an important role during electrotaxis. Here, we show that pretreating human telomerase-immortalized corneal epithelial (hTCEpi) cells with a sialyltransfe… Show more

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Cited by 2 publications
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“…The cells were then imaged every 5 minutes for 4 hours using a Zeiss Observer Z1 microscope with environmental control (37°C and 5% CO 2 ). Cell trajectories were traced as previously described (Feng et al, 2012; Gorelik and Gautreau, 2014; Yang et al, 2019; Le et al, 2023). The “Chemotaxis and Migration Tool” (ibidi GmbH, Gräfelfing, Germany) was used to plot the migration trajectory (Zengel et al, 2011).…”
Section: Methodsmentioning
confidence: 99%
“…The cells were then imaged every 5 minutes for 4 hours using a Zeiss Observer Z1 microscope with environmental control (37°C and 5% CO 2 ). Cell trajectories were traced as previously described (Feng et al, 2012; Gorelik and Gautreau, 2014; Yang et al, 2019; Le et al, 2023). The “Chemotaxis and Migration Tool” (ibidi GmbH, Gräfelfing, Germany) was used to plot the migration trajectory (Zengel et al, 2011).…”
Section: Methodsmentioning
confidence: 99%