Reducing the burden of sexually transmitted infections in resource-limited settings: the role of improved diagnostics

Aledort, Julia E.; Ronald, Allan R.; Rafael, Maria E.; Girosi, Federico; Vickerman, Peter; Blancq, Sylvie M. Le; Landay, Alan; Holmes, King K.; Ridzon, Renée; Hellmann, Nicholas S.; Shea, Molly; Peeling, Rosanna W.

doi:10.1038/nature05447

Cited by 52 publications

(41 citation statements)

References 31 publications

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“…In low-resource settings, many NG infections remain untreated, both because they can remain asymptomatic for years and because rapid and sensitive diagnostics are still lacking (Aledort et al 2006; Bourgeois et al 1998; World Health Organization 2007). Untreated gonorrhea infections can cause pelvic inflammatory disease, infertility, and ectopic pregnancy among women, and additional complications via neonatal transmission.…”

Section: Introductionmentioning

confidence: 99%

“…Untreated gonorrhea infections can cause pelvic inflammatory disease, infertility, and ectopic pregnancy among women, and additional complications via neonatal transmission. NG infections also facilitate HIV transmission (Aledort et al 2006; Fleming and Wasserheit 1999); people with NG infections are up to five times more likely to contract HIV (Wasserheit 1992), and are three times more likely to transmit HIV to someone else (Fleming and Wasserheit 1999). Once diagnosed, this serious but treatable infection is often cured with a one-time dose of appropriate antibiotics.…”

Section: Introductionmentioning

confidence: 99%

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A paperfluidic platform to detect Neisseria gonorrhoeae in clinical samples

Horst

Rosenbohm

Kolluri

et al. 2018

Biomed Microdevices

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Globally, the microbe Neisseria gonorrhoeae (NG) causes 106 million newly documented sexually transmitted infections each year. Once appropriately diagnosed, NG infections can be readily treated with antibiotics, but high-risk patients often do not return to the clinic for treatment if results are not provided at the point of care. A rapid, sensitive molecular diagnostic would help increase NG treatment and reduce the prevalence of this sexually transmitted disease. Here, we report on the design and development of a rapid, highly sensitive, paperfluidic device for point-of-care diagnosis of NG. The device integrates patient swab sample lysis, nucleic acid extraction, thermophilic helicase-dependent amplification (tHDA), an internal amplification control (NGIC), and visual lateral flow detection within an 80 min run time. Limits of NG detection for the NG/NGIC multiplex tHDA assay were determined within the device, and clinical performance was validated retroactively against qPCR-quantified patient samples in a proof-of-concept study. This paperfluidic diagnostic has a clinically relevant limit of detection of 500 NG cells per device with analytical sensitivity down to 10 NG cells per device. In triplicate testing of 40 total urethral and vaginal swab samples, the device had 95% overall sensitivity and 100% specificity, approaching current laboratory-based molecular NG diagnostics. This diagnostic platform could increase access to accurate NG diagnoses to those most in need.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A paperfluidic platform to detect Neisseria gonorrhoeae in clinical samples

Horst

Rosenbohm

Kolluri

et al. 2018

Biomed Microdevices

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show abstract

“…With a need to increase access to treatment, there has been a call to integrate rapid, technologically appropriate syphilis testing at venues with limited diagnostic resources 1 2. Operational requirements to perform the screening tests (RPR or VDRL) are barriers to standard testing in such settings, as refrigeration is required for the storage of reagents, centrifugation for serum separation and an electric rotator for flocculation.…”

Section: Introductionmentioning

confidence: 99%

Syphilis screening among female sex workers in Bangalore, India: comparison of point-of-care testing and traditional serological approaches

Mishra

Naik

Venugopal

et al. 2009

Sexually Transmitted Infections

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“…Het wordt aanbevolen om daarin te investeren in plaats van te wachten op een infrastructuur die de westerse hightech wèl aankan. 15 Een tweede terrein van onderzoek rond diagnostiek richt zich op de verhouding tussen kosten en baten. Is dure diagnostiek altijd wel echt nodig, of zijn er goedkopere alternatieven?…”

Section: Visieunclassified

Duurzame diagnostiek in Afrika

Wit¹

2007

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Reducing the burden of sexually transmitted infections in resource-limited settings: the role of improved diagnostics

Cited by 52 publications

References 31 publications

A paperfluidic platform to detect Neisseria gonorrhoeae in clinical samples

A paperfluidic platform to detect Neisseria gonorrhoeae in clinical samples

Syphilis screening among female sex workers in Bangalore, India: comparison of point-of-care testing and traditional serological approaches

Duurzame diagnostiek in Afrika

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