Reducing the danger of phytopathogen toxins by using an organomineral composition

Abstract

Relevance. Contamination by toxins of phytopathogens or mycotoxins of food and feed raw materials of plant origin poses a serious threat to the production of high-quality and safe agricultural products. Reducing the risks of contamination of plant raw materials with mycotoxins depends on the effective use of biological products to reduce the toxicity of mycotoxins.Methods. Experiments were carried out on primary rat liver cells. Primary liver cells were cultured in DMEM medium in the presence of 10% fetal calf serum at 37OC and 5% CO2. Zearalenone and T-2 toxin were dissolved in a mixture of DMSO and 96% alcohol in a ratio 1:1. Zearalenone T-2 toxin and protective compositions were mixed and aged together for 6 hours and after exposure added to a medium with a cellular monolayer. The concentration of bacterial-based compositions KMBI-3 and KMCI-3 was used for research in three variants: 0.4 mg/ml, 2 mg/ml, 4 mg/ml. After 24 hours of cultivation, the cell layer was evaluated using an inverted microscope according to the following parameters: percentage of surface coverage, cell shape, number of cell aggregates, number of floating cells.Results. A dose-dependent decrease in cell viability was revealed when exposed to zearalenone and T-2 toxin, the most toxic effect was observed at doses from 0.5 х 10-4 and 8.6 х 10-8 M and 2.14 х 10-7 M, respectively. When using the protective composition KMBI-3 at a dose of 4 mg/ml, the least negative effect of zearalenone and T-2 toxin on cell culture was observed. The use of compositions of organomineral origin KMBI-3 helps to increase cell viability when exposed to the toxins zearalenone and T-2 toxin, which indicates the activation of proliferative processes in comparison with the group without the use of drugs. The use of the biopreparation KMBI-3 reduces the pathogenic effect of zearalenone and T-2 toxin on cell culture, increases the resistance of liver cells to the effects of phytopathogen toxins.