Adaptations to sprint interval training (SIT) are observed with brief (≤15-s) work bouts highlighting peak power generation as an important metabolic stimulus. This study examined the effects of manipulating SIT work bout and recovery period duration on energy expenditure (EE) during and postexercise, as well as postexercise fat oxidation rates. Nine active males completed a resting control session (CTRL) and 3 SIT sessions in randomized order: (i) 30:240 (4 × 30-s bouts, 240-s recovery); (ii) 15:120 (8 × 15-s bouts, 120-s recovery); (3) 5:40 (24 × 5-s bouts, 40-s recovery). Protocols were matched for the total duration of work (2 min) and recovery (16 min), as well as the work-to-recovery ratio (1:8 s). EE and fat oxidation rates were derived from gas exchange measured before, during, and for 3 h postexercise. All protocols increased EE versus CTRL (P < 0.001). Exercise EE was greater (P < 0.001) with 5:40 (209 kcal) versus both 15:120 (163 kcal) and 30:240 (138 kcal), while 15:120 was also greater (P < 0.001) than 30:240. Postexercise EE was greater (P = 0.014) with 15:120 (313 kcal) versus 5:40 (294 kcal), though both were similar (P > 0.077) to 30:240 (309 kcal). Postexercise fat oxidation was similar (P = 0.650) after 15:120 (0.104 g·min −1 ) and 30:240 (0.116 g·min −1 ) and both were greater (P < 0.030) than 5:40 (0.072 g·min −1 ) and CTRL (0.049 g·min −1 ). In conclusion, shorter SIT work bouts that target peak power generation increase exercise EE without compromising postexercise EE, though longer bouts promote greater postexercise fat utilization.Key words: high-intensity interval training, energy expenditure, excess postexercise oxygen consumption, fat oxidation, repeated sprint exercise, peak power generation. Mots-clés : entraînement par intervalle d'intensité élevée, dépense énergétique, consommation d'oxygène postexercice en surplus, oxydation des graisses, exercice de sprint répété, production de puissance de pointe.