The utilization of nitrogen (N) fertilizer in peatlands, with the aim of increasing crop growth and production, is also reported to increase carbon dioxide (CO 2 ) emissions. The application of coal fly ash (CFA) to soil may change soil physico-chemical characteristics, thereby influence carbon mineralization, but its effect on CO 2 production is not yet clear. Consequently, the purpose of this study was to quantify the CO 2 production of tropical peatlands that received N fertilizer and CFA. In the laboratory experiment, CFA equivalent to the application of 150 Mg•ha −1 in the field was added to peatlands with and without N fertilizer. These mixtures were then incubated at 70% waterfilled pore space (WFPS) for 30 days at room temperature. Carbon mineralization was measured on a 5-day basis, while several chemical characteristics of treated peatlands, including pH, hot water-soluble C, exchangeable-Ca, -Mg, -Fe, and -Al were measured at the conclusion of the incubation period. This study identified that N fertilizer application increased the CO 2 production of tropical peatlands from 29.25 g•kg −1 to 37.12 g•kg −1 . Furthermore, the application of CFA on tropical peatlands reduced CO 2 production of tropical peatlands with and without N fertilizer. Decreasing the amount of hot water-soluble carbon from peatlands may account for the reduced CO 2 production of peatlands with CFA. The study also showed that exchangeable-Ca, -Mg, -Fe, and -Al increased in peatlands with CFA application, and these multivalent cations were also attributed to a reduction of CO 2 production. In conclusion, the negative effects of N fertilizer application on peatlands in increasing CO 2 emission may be reduced by the application of CFA.