Reduction in Cold-Induced Sweetening by Cas9 Endonuclease-Mediated Knockout of the POTATO VACUOLAR INVERTASE 1 Gene in the Cultivar ‘Symfonia’

Egorova, Anastasiya A.; Zykova, Tatyana E.; Kostina, Nina E.; Saboiev, Izatsho A.; Koloshina, Kristina A.; Filipenko, Elena A.; Hoffie, Iris; Hertig, Christian; Hiekel, Stefan; Kumlehn, Jochen; Kochetov, Alex V.; Gerasimova, Sophia V.

doi:10.1007/s11540-024-09800-6

Potato Res.

2024

DOI: 10.1007/s11540-024-09800-6

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Reduction in Cold-Induced Sweetening by Cas9 Endonuclease-Mediated Knockout of the POTATO VACUOLAR INVERTASE 1 Gene in the Cultivar ‘Symfonia’

Anastasiya A. Egorova,

Tatyana E. Zykova,

Nina E. Kostina

et al.

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Accumulation of Anthocyanin in the Aleurone of Barley Grains by Targeted Restoration of the MYC2 Gene

Egorova,

Zykova,

Hertig

et al. 2024

IJMS

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Blue barley grain pigmentation results from anthocyanin accumulation in the aleurone layer. Anthocyanins are known for their beneficial effects on human health. The gene encoding the MYELOCYTOMATOSIS 2 (MYC2) transcription factor is potentially responsible for the blue coloration of the aleurone. In non-pigmented barley, a single nucleotide insertion in this gene causes a frameshift mutation with a premature stop codon. It was hypothesized that restoring the MYC2 reading frame could activate anthocyanin accumulation in the aleurone. Using a targeted mutagenesis approach in the present study, the reading frame of MYC2 was restored in the non-pigmented cultivar Golden Promise. Genetic constructs harboring cas9 and gRNA expression units were developed, pre-validated in protoplasts, and then functional MYC2 alleles were generated at the plant level via Agrobacterium-mediated transformation. Anthocyanin accumulation in the aleurone layer of grains from these mutants was confirmed through microscopy and chemical analysis. The expression of anthocyanin biosynthesis genes was analyzed, revealing that the restoration of MYC2 led to increased transcript levels of F3H and ANS genes. These results confirm the critical role of the MYC2 transcription factor in the blue aleurone trait and provide a biotechnological solution for enriching barley grain with anthocyanins.

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Accumulation of Anthocyanin in the Aleurone of Barley Grains by Targeted Restoration of the MYC2 Gene

Egorova,

Zykova,

Hertig

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

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Reduction in Cold-Induced Sweetening by Cas9 Endonuclease-Mediated Knockout of the POTATO VACUOLAR INVERTASE 1 Gene in the Cultivar ‘Symfonia’

Cited by 1 publication

References 47 publications

Accumulation of Anthocyanin in the Aleurone of Barley Grains by Targeted Restoration of the MYC2 Gene

Accumulation of Anthocyanin in the Aleurone of Barley Grains by Targeted Restoration of the MYC2 Gene

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