Reduction in lignin content and increase in the antioxidant capacity of corn and sugarcane silages treated with an enzymatic complex produced by white rot fungus

Abstract

The objective was to evaluate the effect of the addition of 0, 10, 20, and 30 mg.kg-1 of natural matter of a lignocellulosic enzymatic complex produced by the white rot fungus on the chemical composition, cumulative gas production in vitro, and antioxidant compounds of corn and sugarcane silages. After being chopped and treated with the enzymatic complex, the plants were packed in vacuum-sealed bags. After 60 days, the mini silos were opened and the samples were dried in a forced ventilation oven at 55˚C for analysis of the proposed parameters. The experiment was conducted in a completely randomized design with four replicates per treatment. In the corn silage, there was a linear reduction in the lignin concentration. In the sugarcane silage showed a reduction of 12% in the lignin concentration, a linear reduction in the hemicellulose content, and a decrease of 8% in the cellulose concentration compared to the control treatment. The lignin monomers had linear increases in the syringyl:guaiacil ratio. This reflected on significant increases in the concentration of the non-fibrous carbohydrates and the A + B1 fraction of the carbohydrates, and a reduction in the C fraction. The in vitro gas production increased, the time of colonization and initiation of in vitro fermentation linearly decreased in both silages. The phenolic compounds and the antioxidant capacity increased linearly with the addition of the enzymes in both silages. The addition of the lignocellulolytic enzymes to the silages caused changes in the cell wall, resulting in improvements in the in vitro fermentative parameters, besides the additional effect on the antioxidant capacity. There was an effect of the addition of the enzymes on the evaluated fodder, and the best concentration was, on average, 20 mg kg-1 MN for corn silage and 10 mg kg-1 NM for sugarcane silage.

A comparative study of greener alternatives for nanocellulose production from sugarcane bagasse

“…An increase in S/G ratio indicates a reduction in the guaiacyl content and therefore we speculate that laccase in combination with GO can degrade the more condensed guaiacyl linkages as compared to laccase with ABTS. An increase in S/G values with a reduction in lignin has also been reported in literature (Chanoca et al 2019 ; Machado et al 2020 ).…”

Ferulic Acid Esterase Producing Lactobacillus johnsonii from Goat Feces as Corn Silage Inoculants

“…The higher FA concentration in ETC175-inoculated silages can be beneficial, as a previous report indicated an improvement in meat quality by the addition of 30 mg. kg −1 of FA to the diet of steers [13]. Interestingly, no correlation could be detected between FA concentration, TPC, and DPPH results in our experimental silages in contrast to previous reports on corn silages treated with fibrolytic enzymes [78] or alfalfa silages treated with FAE+ LAB [14]; however, in many cases, different types of determinations and different extraction methods were performed. It is proposed that FA reacts with increasing scavenging activity, but this could not be detected in the present experiment [14].…”

“…It is proposed that FA reacts with increasing scavenging activity, but this could not be detected in the present experiment [14]. The TPC analyzed in this work was generally lower than that previously reported for corn silages [78], which, in that case, correlated with the amount of fibrolytic enzymes applied. The TPC content was negatively correlated with the digestibility measures by Taboada et al [79], as observed in the CRL1446-inoculated group, but not in the rest of the experimental groups of this work.…”

Chemical composition and gas production kinetic parameters of sweet potato vine waste silage after preserved for short and prolonged periods

“…The low variation is additional evidence that the ensiling process was efficient and that the ensiled material kept the quality of the fresh forage. Machado et al (2020) discuss how, during the process of cell wall digestion by microorganisms in the rumen, lignin plays a significant role as a constraining element. This is primarily attributed to its strong connection with cellulose and, more notably, with hemicellulose.…”