Reduction in Peyronie's-like plaque size using a vacuum erection device in a rat model of Peyronie's disease via the TGF-β/SMAD signalling pathway

Li, Jinhong; Wang, Shuzhen; Qin, Feng; Zhu, Meng-Li; You, Xuanhe; Wu, Changjing; Fu, Fudong; Yuan, Jiuhong

doi:10.1111/and.13051

Cited by 21 publications

(24 citation statements)

References 28 publications

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“…Following routine dehydration and paraffin embedding, samples were cut into 5‐µm sections. Tissue slides with cross‐sections of the corpus cavernosum were deparaffinized and rehydrated for analysis 19,22 …”

Section: Methodsmentioning

confidence: 99%

Characteristics of penile growth in pubertal rats and a non‐invasive method to lengthen the penis

et al. 2020

Andrology

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Background Males with short penises may suffer from sexual dysfunction and psychological problems. However, currently, managing short penis is a huge challenge. Objectives To explore whether inhibition of lysyl oxidase (LOX) activity (anti‐LOX) combined with a vacuum device could lengthen the penis of pubertal rat. Materials and Methods Male rats of different ages were purchased, their exposed penile lengths and weights were measured, and protein expression and lysyl oxidase activity in the corpus cavernosum were analyzed. Fifteen‐day‐old rats were then purchased and divided into six groups: control, Anti‐lysyl oxidase, −200 mm Hg (vacuum device under −200 mm Hg value), −200 mm Hg + Anti‐lysyl oxidase, −300 mm Hg, and −300 mm Hg + Anti‐lysyl oxidase groups. After the intervention duration of 7 weeks, rats' penile length was measured and erectile function was assessed. The corpus cavernosum was harvested for histopathology and molecular assessments. Results Exposed penile length and weight significantly increased with age, especially between 4 and 8 weeks. Both the protein expression and lysyl oxidase activity in corpus cavernosum were the highest at 2 weeks; however, they quickly decreased with age and slowly declined after 8 weeks. Anti‐lysyl oxidase significantly increased the penile length by 10.79% over controlled rats, −200 mm Hg + Anti‐lysyl oxidase lengthened it by 14.05%, and −300 mm Hg + Anti‐lysyl oxidase increased it by 19.84%. Anti‐lysyl oxidase significantly reduced lysyl oxidase activity to decrease pyridinoline concentration; however, it did not change desmosine (P = .28), hydroxyproline (P = .14), and total elastin (P = .06) levels. Anti‐lysyl oxidase with or without a vacuum device did not diminish erectile function or impair the normal microstructure of corpus cavernosum. Discussion and Conclusion The rats' penile growth peaks occurred between 4 and 8 weeks. Anti‐lysyl oxidase with a vacuum device promoted penile lengthening by inhibiting pyridinoline production to induce tunica albuginea remodeling. The penile lengthening effect was more obvious in pubertal rats than the adult rats. None of the procedures decreased erectile function.

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Section: Methodsmentioning

confidence: 99%

Characteristics of penile growth in pubertal rats and a non‐invasive method to lengthen the penis

et al. 2020

Andrology

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“…Peyronie's disease is a penile fibrotic disorder characterised by the development of plaques in the tunica albuginea (Li et al., 2018). In previous studies, the beneficial effects of stem cells intratunical administration have been investigated as an effective modality (Castiglione et al., 2013; Gokce et al., 2014, 2015).…”

Section: Discussionmentioning

confidence: 99%

Intratunical injection of human urine‐derived stem cells derived exosomes prevents fibrosis and improves erectile function in a rat model of Peyronie's disease

et al. 2020

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We aimed to evaluate the effects of intratunical injection of exosomes derived from human urine‐derived stem cells (USC‐exo) on plaque formation and erectile function in a transforming growth factor‐β1 (TGF‐β1) induced Peyronie's disease (PD) rat model. Twenty‐four SD rats were randomly assigned equally to three groups: (I) Sham group (50 μl phosphate‐buffered saline [PBS] injected into the tunica albuginea [TA]), (II) PD group (0.5 μg TGF‐β1 in 50 μl PBS injected into the TA) and (III) USC‐exo group (0.5 ug TGF‐β1 plus 100 μg USC‐exo injected into the TA at the same day). The maximum intracavernous pressure (ICPmax) and mean arterial pressure (MAP) of each group were evaluated 4 weeks after injection. The plaque formation, fibrosis, matrix metalloproteinases (MMPs) and tissue inhibitor of MMPs (TIMPs) in the TA were evaluated. Four weeks after injection, USC‐exo group showed more significantly enhanced ICPmax and ICPmax/MAP than PD group (p < .05). USC‐exo could significantly ameliorate the TA fibrosis that could be associated with the inhibition of transdifferentiation of fibroblasts into myofibroblasts, decreased expression of TIMPs (TIMP‐1, 2, 3) and increased activity of MMPs (MMP‐1, 3, 9) in the TA. According to these findings, USC‐exo can be a new candidate for the prevention of PD.

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“… 28 33 The cavernous nerve was stimulated electrically at 5.0 V, 20.0 Hz, pulse width of 5.0 ms, for 50 s. 28 29 33 Intracavernous pressure (ICP) and arterial pressure were simultaneously monitored with a BL420 biofunctional experiment system (TME Technology Co. Ltd. Chengdu, China). 28 33 The maximum ICP/mean arterial pressure (MAP) ratio was calculated and analyzed. After the erectile function measurement, the exposed penis was cut at the junction of the urethral bulb and corpus cavernosum and then the excised penile weight was measured.…”

Section: Methodsmentioning

confidence: 99%

Anti-lysyl oxidase combined with a vacuum device induces penile lengthening by remodeling the tunica albuginea

et al. 2020

Asian J Androl

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This study aimed to explore whether and how anti-lysyl oxidase (anti-LOX) combined with a vacuum device (VD) could promote penile lengthening and to evaluate the effect on erectile function. This study was performed on four groups of adult rats: control, anti-LOX, VD (negative pressure value of −300 mmHg), and anti-LOX + VD. Penile length was measured by a modified VD method and verified on exposed length data. Intracavernous pressure (ICP) and maximum ICP/mean arterial pressure (MAP) ratio were recorded to assess erectile function. For corpus cavernosum, LOX activity and concentrations of pyridinoline, desmosine, hydroxyproline, and elastin were analyzed; transmission electron microscope and Hart's elastin staining were performed to monitor microstructural changes. Anti-LOX and VD significantly lengthened the penis by 10.8% (3.75 mm) and 8.2% (2.48 mm) compared with the control group, respectively, while anti-LOX + VD achieved the longest penile size (40.58 ± 0.40 mm) which was 17.4% longer than the control group (34.58 ± 0.54 mm). After 1-week washout, no penile retraction was observed. Meanwhile, exposed penile length data confirmed that the penis in the anti-LOX + VD group was also significantly longer. Anti-LOX inhibited LOX activity to reduce pyridinoline level, which led the penile tunica albuginea remodeling. However, it had no effect on hydroxyproline, desmosine, and elastin levels. Moreover, anti-LOX had no impact on erectile function, which was determined by ICP and ICP/MAP ratio. These results suggest that anti-LOX elongates the penis by reducing pyridinoline, which induces tunica albuginea remodeling. This lengthening effect was more obvious when combined with a VD. All procedures had no impact on erectile function.

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Reduction in Peyronie's-like plaque size using a vacuum erection device in a rat model of Peyronie's disease via the TGF-β/SMAD signalling pathway

Cited by 21 publications

References 28 publications

Characteristics of penile growth in pubertal rats and a non‐invasive method to lengthen the penis

Characteristics of penile growth in pubertal rats and a non‐invasive method to lengthen the penis

Intratunical injection of human urine‐derived stem cells derived exosomes prevents fibrosis and improves erectile function in a rat model of Peyronie's disease

Anti-lysyl oxidase combined with a vacuum device induces penile lengthening by remodeling the tunica albuginea

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