Reduction of abscisic acid content and induction of sprouting in potato,Solanum tuberosum L., by thidiazuron

Ji, Z. L.; Wang, S. Y.

doi:10.1007/bf02054160

Cited by 30 publications

(16 citation statements)

References 15 publications

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“…For example, suppression of sprout growth in eyes excised from nondormant tubers required repeated or continuous exposure to relatively high concentrations of ABA (van Es and Hartmans, 1969). In some cases, a modest stimulation of sprout growth by exogenous ABA has been observed in nondormant tubers (Ji and Wang, 1988). As described above, exogenous ABA was effective at extemal concentrations that restored internal ABA levels to values similar to those found in untreated tubers.…”

Section: Dlscusslonmentioning

confidence: 67%

Role of Endogenous Abscisic Acid in Potato Microtuber Dormancy

Suttle

Hultstrand

1994

Plant Physiol.

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Potato (Solanum tuberosum L. cv Russet Burbank) microtubers generated in vitro from single-node explants contained substantial amounts (approximately 250 pmol/g fresh weight) of free abscisic acid (ABA) and were completely dormant for a minimum of 12 weeks. Microtubers that developed in the presence of 10 NM fluridone (FLD) contained considerably reduced amounts (approximately 5-25 pmol/g fresh weight) of free ABA and exhibited a precocious loss of dormancy. lnclusion of exogenous racemic ABA in the FLD-containing medium suppressed the premature sprouting of these microtubers in a dose-dependent manner. At a concentration of 50 p~, exogenous ABA restored internal ABA levels to control values and completely inhibited FLD-induced precocious sprouting. Exogenous jasmonic acid was ineffective in suppressing FLD-induced sprouting. Application of FLD to preformed, fully dormant microtubers also resulted in a reduction in internal ABA content and precocious sprouting. These results indicate that endogenous ABA is essential for the induction and maintenance of potato microtuber dormancy.

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Section: Dlscusslonmentioning

confidence: 67%

Role of Endogenous Abscisic Acid in Potato Microtuber Dormancy

Suttle

Hultstrand

1994

Plant Physiol.

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“…Evidence for a causal relationship between traits such as ABA content and potato dormancy has usually been based on correlative evidence from observations or treatments within a single genotype (Ji and Wang, 1988; Presence of More than One QTL per Chromosome Cvikrová et al, 1994;Suttle and-Hultstrand, 1994). As noted by Lebreton et al (1995), identifying the location of…”

Section: Coincidence With Qtls For Other Traitsmentioning

confidence: 99%

Evidence from Polygene Mapping for a Causal Relationship between Potato Tuber Dormancy and Abscisic Acid Content

et al. 1997

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In previous studies polygene mapping of a backcross population derived from haploid potato (Solanum tuberosum) and a diploid wild species (Solanum berthaultiij showed at least eight quantitative trait loci (QTLs) associated with tuber dormancy. The same population was mapped for abscisic acid (ABA) content in tubers so that any QTLs identified could be compared with those detected previously. At least three distinct loci on three chromosomes (2, 4, and 7) were associated with variation in ABA content. One of the QTLs was detected only as a main (single locus) effect, and two QTLs were found through two-locus interaction analysis (epistasis). Interaction between QTLs at markers TG234 (chromosome 2) and TG755 (chromosome 4) explained 20% of total phenotypic variante for this trait. The interaction closely resembled one previously detected for dormancy, suggesting an association between high ABA content and long tuber dormancy. Although relationships between ABA leve1 and dormancy could be demonstrated through polygene mapping, there was no indication of a relationship between these traits when they were subjected to a conventional correlation test. This illustrates the usefulness of polygene mapping as a tool to identify possible associations between hormone levels and plant development.A well-tested approach for understanding growth and development is the analysis of differences between two phenotypes that contain different alleles of a single major gene. For example, analyses of dwarf and wild-type siblings have revealed hormonal differences associated with the mutant genotype (Reid and Howell, 1995). Although this approach has been productive, a limitation is that the control of many developmental processes is polygenic, i.e. inheritance is quantitative rather than qualitative. Thus, although it is interesting to know what physiological effect was produced by a dwarf mutation, it might be much more informative to learn about the physiological effects pro- duced by the polygenes that control plant height. In the past the study of quantitative inheritance was so difficult that it yielded only very generalized information, and little could be learned that would elucidate the physiology of growth and development. The situation has changed dramatically with the availability of new methods for mapping polygenes (Tanksley et al., 1989;Tanksley, 1993;Young, 1993). The new mapping methods rely on DNA-based genetic markers, such as RFLP markers. Through such mapping, a number of QTLs may be found that are associated with the control of a trait such as plant height. A QTL denotes a region of chromosome linked to the marker gene that has a significant effect on the quantitative trait (Tanksley, 1993).With the availability of this tool for mapping quantitative traits, polygenes can be used to study the physiology of growth and development in a manner analogous to, but more powerful than, the way single genes have been used in the past. Once the QTLs have been detected that are associated with the control of a given stage of...

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“…Endogenous plant hormones and their relative balance within the tuber are suggested to regulate endodormancy and sprouting (Turnbull and Hanke, 1985;Ji and Wang, 1988;Suttle, 2004aSuttle, , 2009Sorce et al, 2009;Hartmann et al, 2011). Ethylene and abscisic acid have been associated with the onset and maintenance of tuber dormancy (Suttle and Hultstrand, 1994), and molecular analysis has indicated that genes associated with the anabolic and catabolic metabolism of abscisic acid correlate with dormancy in potato meristems and tubers (Simko et al, 1997;Ewing et al, 2004;DestefanoBeltrán et al, 2006b;Campbell et al, 2010).…”

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confidence: 99%

Release of Apical Dominance in Potato Tuber Is Accompanied by Programmed Cell Death in the Apical Bud Meristem

et al. 2012

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Potato (Solanum tuberosum) tuber, a swollen underground stem, is used as a model system for the study of dormancy release and sprouting. Natural dormancy release, at room temperature, is initiated by tuber apical bud meristem (TAB-meristem) sprouting characterized by apical dominance (AD). Dormancy is shortened by treatments such as bromoethane (BE), which mimics the phenotype of dormancy release in cold storage by inducing early sprouting of several buds simultaneously. We studied the mechanisms governing TAB-meristem dominance release. TAB-meristem decapitation resulted in the development of increasing numbers of axillary buds with time in storage, suggesting the need for autonomous dormancy release of each bud prior to control by the apical bud. Hallmarks of programmed cell death (PCD) were identified in the TAB-meristems during normal growth, and these were more extensive when AD was lost following either extended cold storage or BE treatment. Hallmarks included DNA fragmentation, induced gene expression of vacuolar processing enzyme1 (VPE1), and elevated VPE activity. VPE1 protein was semipurified from BE-treated apical buds, and its endogenous activity was fully inhibited by a cysteinyl aspartate-specific protease-1-specific inhibitor N-Acetyl-Tyr-Val-Ala-Asp-CHO (Ac-YVAD-CHO). Transmission electron microscopy further revealed PCD-related structural alterations in the TAB-meristem of BE-treated tubers: a knob-like body in the vacuole, development of cytoplasmic vesicles, and budding-like nuclear segmentations. Treatment of tubers with BE and then VPE inhibitor induced faster growth and recovered AD in detached and nondetached apical buds, respectively. We hypothesize that PCD occurrence is associated with the weakening of tuber AD, allowing early sprouting of mature lateral buds.

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Reduction of abscisic acid content and induction of sprouting in potato,Solanum tuberosum L., by thidiazuron

Cited by 30 publications

References 15 publications

Role of Endogenous Abscisic Acid in Potato Microtuber Dormancy

Role of Endogenous Abscisic Acid in Potato Microtuber Dormancy

Evidence from Polygene Mapping for a Causal Relationship between Potato Tuber Dormancy and Abscisic Acid Content

Release of Apical Dominance in Potato Tuber Is Accompanied by Programmed Cell Death in the Apical Bud Meristem

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