Reduction of adverse citrate reactions during autologous large‐volume PBPC apheresis by continuous infusion of calcium‐gluconate

Buchta, Christoph; Macher, Maria; Bieglmayer, Christian; Höcker, P; Dettke, Markus

doi:10.1046/j.1537-2995.2003.00571.x

Cited by 61 publications

(62 citation statements)

References 28 publications

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“…In agreement with previous reports [33,48], we did not observe increase in number of adverse events during LVL collection. Buchta et al [49] showed that prophylactic calcium infusion during LVL reduced the incidence of citrate-related symptoms without affecting the technical performance or the number of CD34+ cells collected, as was confirmed by our results. Our study also confirmed marked platelet decrease after processing each TBV with halved platelet count after LVL [3,48].…”

Section: Bojanic 17supporting

confidence: 90%

Large volume leukapheresis: Efficacy and safety of processing patient’s total blood volume six times

Bojanić

Dubravčić

Batinić

et al. 2011

Transfusion and Apheresis Science

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Section: Bojanic 17supporting

confidence: 90%

Large volume leukapheresis: Efficacy and safety of processing patient’s total blood volume six times

Bojanić

Dubravčić

Batinić

et al. 2011

Transfusion and Apheresis Science

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“…Apheresis-related key parameters are shown in Table II. During LVL, all patients received a bypass infusion of 8.92 mmol (375.5 mg) calcium diluted in 500 mL of saline to avoid adverse citrate effects, as described previously [15].…”

Section: Mnc Collection With the Cobe Spectra Auto Pbscmentioning

confidence: 99%

Combination of Cobe AutoPBSC and Gambro Elutra as a platform for monocyte enrichment in dendritic cell (DC) therapy: Clinical study

Chen¹,

Hoecker²,

Zeng³

et al. 2008

J of Clinical Apheresis

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Monocytes are a common source for generating dendritic cells (DCs). The aim of the present study was to evaluate the efficiency of a platform for monocyte collection and enrichment in a clinical setting. The platform was based on the combination of two semiautomated devices; the Cobe Spectra Auto PBSC for mononuclear cells (MNC) collection followed by counterflow elutriation for monocyte enrichment (Gambro BCT Elutra). Twenty-four patients with various types of epithelial cancer participated in the study. MNC collections were first performed as large volume leukapheresis (LVL). Subsequently, MNC products were processed with an elutriation system for monocyte isolation. LVL resulted in the collection of MNC at a median of 8.1 x 10(9) cells, containing of 31.4% monocytes. A similar efficacy was also shown in patients with lower peripheral blood counts. Elutriation of the MNC product with the Cobe Elutra device resulted in the enrichment of monocytes at a median of 2.7 x 10(9) cells, with a recovery of 80.2% and a purity of 90.7%. These monocytes were then successfully developed into DCs for clinical therapy after in vitro manipulation. These data suggest that the combination of the Cobe Spectra Auto PBSC and the Gambro BCT Elutra is an effective platform for monocyte enrichment in clinical practice according to GCP standards and GMP guidelines, and can be easily implemented in the clinical routine under current DC protocols.

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“…Hypocalcemia is a toxicity of citrate administration 18,19 and is thought to be more common in pediatric patients receiving citrate than adults. 12,20 Hypocalcemia was screened for in 77% of the donors and measured in 4% (three with ionized Ca 2 þ o0.8 mmol/l (CTC grade 4), one between 0.8 and 0.9 mmol/l (CTC grade 3), and three between 0.9 and 1.0 mmol/l (CTC grade 2)).…”

Section: Complications Of Growth Factor Administration and Apheresismentioning

confidence: 99%

Safety and efficacy of allogeneic PBSC collection in normal pediatric donors: The Pediatric Blood and Marrow Transplant Consortium Experience (PBMTC) 1996–2003

Pulsipher

Levine

Hayashi

et al. 2004

Bone Marrow Transplant

101

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Summary:The use of peripheral blood stem cells (PBSC) for allogeneic transplants in adults has greatly increased. This trend is reflected in pediatrics, where healthy children increasingly are donating PBSC or donor lymphocyte infusion (DLI) via apheresis for use by ill siblings. There is a potential concern that the risks of PBSC collection may differ for pediatric donors. However, no large studies have assessed safety issues in this population. To address this need, we reviewed 218 (213 PBSC, five DLI) collections in 201 normal pediatric donors (8 months to 17 years, median 11.8 years) at 22 institutions in the Pediatric Blood and Marrow Transplant Consortium. Donors received a median of 4 days of growth factor, and mean collection yield was 9.1 Â 10 6 CD34 þ cells/kg recipient weight. Younger age, days of apheresis, and male gender predicted increased yield of CD34 þ cells/kg donor weight. Growth factor-induced pain was mild and reported in less than 15% of patients. Most donors o20 kg (23/25, 92%) required PRBC priming of the apheresis machine. This experience with over 200 collections demonstrates that PBSC collection is safe in normal pediatric donors and desired CD34 cell yields are easily achieved. Younger children utilize more medical resources and children o20 kg usually require a single blood product exposure.

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Reduction of adverse citrate reactions during autologous large‐volume PBPC apheresis by continuous infusion of calcium‐gluconate

Abstract: These results indicate that continuous support of calcium-gluconate during LVL is an effective means of reducing the incidence of citrate-related symptoms and improving subjective tolerance toward LVL, without affecting the technical performance or the number of CD34+ cells collected.

Cited by 61 publications

References 28 publications

Large volume leukapheresis: Efficacy and safety of processing patient’s total blood volume six times

Large volume leukapheresis: Efficacy and safety of processing patient’s total blood volume six times

Combination of Cobe AutoPBSC and Gambro Elutra as a platform for monocyte enrichment in dendritic cell (DC) therapy: Clinical study

Safety and efficacy of allogeneic PBSC collection in normal pediatric donors: The Pediatric Blood and Marrow Transplant Consortium Experience (PBMTC) 1996–2003

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