Reduction of apoptosis through the mitochondrial pathway by the administration of acetyl-l-carnitine to mouse fibroblasts in culture

Pillich, Rudolf T.; Scarsella, G.; Risuleo, Gianfranco

doi:10.1016/j.yexcr.2005.01.019

Cited by 67 publications

(44 citation statements)

References 34 publications

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“…As expected in the animals treated also with Lcarnitine the enzyme immuno-band is significantly reduced. This validates the idea that the drug exerts a strong cytoprotective action during the re-oxigenation and reperfusion process (Mutomba et al, 2000;Di Marzio et al, 1997;Pillich et al, 2005). Similar results were obtained 24 hour after the treatment.…”

Section: Oxidative Stress As a Consequence Of The Acute Ocular Hypertsupporting

confidence: 86%

Role of the Matrix Metallo-Proteinases in the Cellular Re-Modeling in a Glaucoma Model System in Rat

Seta¹,

Calandrella²,

Berardi³

et al. 2011

Glaucoma - Current Clinical and Research Aspects

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Section: Oxidative Stress As a Consequence Of The Acute Ocular Hypertsupporting

confidence: 86%

Role of the Matrix Metallo-Proteinases in the Cellular Re-Modeling in a Glaucoma Model System in Rat

Seta¹,

Calandrella²,

Berardi³

et al. 2011

Glaucoma - Current Clinical and Research Aspects

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“…It is a short-chain ester derivative of L -carnitine and it is synthesized in the human brain, liver and kidney. It is a neuroprotective and antiapoptotic agent for various cell types [14,15,16,17,18,19,20,21]. …”

Section: Discussionmentioning

confidence: 99%

“…It is a well-known neuroprotective agent and is also protective against CDDP-induced neurotoxicity [14,15,16,17]. Also, it has antiapoptotic effects on various cell types [18,19,20,21]. …”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Effect of Acetyl <i>L</i>-Carnitine on Experimental Cisplatin Ototoxicity and Neurotoxicity

Güneş

Kırkım²,

Kolatan³

et al. 2011

Chemotherapy

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Introduction: Cisplatin (CDDP) is an effective and widely used chemotherapeutic agent for pediatric tumors, and ototoxicity is one of the dose-limiting side effects. Objective: It was the aim of our study to investigate the effect of acetyl L-carnitine (ALCAR) on experimental CDDP ototoxicity by audiologic tests, histomorphologic, immunohistochemical and ultrastructural examinations and to investigate the apoptotic pathways. Materials and Methods: Wistar albino rats (n = 28) were studied. Baseline audiological tests were performed in 4 groups: group 1, control; group 2, ALCAR; group 3, CDDP; group 4, CDDP + ALCAR-administered rats. Control audiological tests were performed on the 3rd day, and then the rats were sacrificed. Ear and brain specimens were examined by transmission electron microscopy, and caspase 3, 8 and 9 activities were investigated. Results: The CDDP-administered rats showed significant auditory brainstem response threshold shifts using all stimuli (clicks, 6-kHz and 8-kHz tone burst) compared with the control groups. The CDDP + ALCAR-administered rats showed significant auditory brainstem response threshold shifts by only click stimuli compared with the control groups. In the brain, spiral ganglion and organ of Corti, ultrastructural damage was prominent in group 3; the number of TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling)-positive cells and caspase 3, 8 and 9 immunostaining cells was significantly high in group 3. Conclusion: ALCAR improves CDDP-induced auditory impairment, and also antioxidative and antiapoptotic properties of ALCAR on CDDP ototoxicity were supported by the findings.

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“…This was the case of the synchronization procedure by which 82% of the cells are set in G0/G1 phase. The synchronization treatment by serum deprivation induces apoptosis by itself in a fraction of cells, chiefly via mitochondrial signaling pathways (Bialik et al 1999;Pillich et al 2005). However, the vital state of population as a whole is not seriously impaired because the fraction of vital cells remains around 70% for the first 12 h after the synchronization treatment (see Fig.…”

Section: Discussionmentioning

confidence: 99%

Experimental testing of a mathematical model relevant to the extrinsic pathway of apoptosis

Pace

Bellizzi

Giordano

et al. 2010

Cell Stress and Chaperones

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Apoptosis is a programmed cell death process, whose complexity led researchers to build mathematical models that could help to identify its crucial steps. In previous works, we theoretically analyzed and numerically simulated a model that describes a pathway from an external stimulus to caspase-3 activation. Here, the results of experiments performed on populations of synchronized cells treated with the inducer Apo2L/TRAIL are reported and are compared with model predictions. In particular, we have compared in vitro and in silico results relevant to the time evolutions of caspase-3 and caspase-8 activities, as well as of the dead cells fractions. In addition, the effect of the BAR gene silencing was evaluated. Caspase-3 activation and cell death is faster in silenced than in nonsilenced cells, thus confirming previous simulation results. Interestingly, Apo2L/TRAIL treatment in itself reduces the BAR gene expression. The qualitative agreement between model predictions and cell cultures behavior suggests that the model captures the essential features of the biological process and could be a tool in further studies of caspases activation. In this manuscript, we report the results of in vitro experiments aimed at revealing the dynamics of caspase activation in a cell population. A qualitative agreement between these results and a mathematical model describing a pathway from an external stimulus to caspase-3 activation was obtained, thus showing that the model captures the essential features of the biological process and may be a reliable tool in further studies of caspase activation.

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Reduction of apoptosis through the mitochondrial pathway by the administration of acetyl-l-carnitine to mouse fibroblasts in culture

Cited by 67 publications

References 34 publications

Role of the Matrix Metallo-Proteinases in the Cellular Re-Modeling in a Glaucoma Model System in Rat

Role of the Matrix Metallo-Proteinases in the Cellular Re-Modeling in a Glaucoma Model System in Rat

Evaluation of the Effect of Acetyl <i>L</i>-Carnitine on Experimental Cisplatin Ototoxicity and Neurotoxicity

Experimental testing of a mathematical model relevant to the extrinsic pathway of apoptosis

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