Reduction of CAII expression in gastric cancer: Correlation with invasion and metastasis

Abstract

Objective: Human carbonic anhydrases II (CAII) gene plays an important role in different cancer. However, its relevance to gastric cancer (GC) remains unclear. In the present study, we aimed to investigate the expression of CAII in GC and explore its correlation with some clinicopathologic characteristics of GC.Methods: The expression of CAII in 20 specimens of normal gastric mucosa, 38 specimens of intraepithelial neoplasia and 112 specimens of gastric carcinoma were detected by immunohistochemical techniques. Survival in GC with CAII expression was studied.Results: The positive rate of CAII protein in normal gastric mucosa was significantly higher than that in intraepithelial neoplasia and gastric carcinoma (100% vs. 63.16% and 28.57%, P<0.001). The positive rate of CAII protein was significantly higher in gastric carcinoma at early stages than that at advanced stages (70.0% vs. 19.57%, P<0.001). The positive rate of CAII protein was significantly lower in gastric carcinoma with lymph node metastases than that without lymph node metastases (10.81% vs. 37.33%, P<0.05). Furthermore, the positive rate of CAII protein was significantly lower in poorly-differentiated gastric carcinoma than in moderately-or well-differentiated gastric carcinoma (15.94% vs. 31.03% or 60.00%, P<0.05). Moreover, CAII expression was not related with sex, age and tumor size. The patients with CAII-positive tumors showed a better survival rate than those with CAII-negative tumors (P=0.024, log-rank test).Conclusion: CAII expression was related with stages and lymph node metastases in gastric carcinoma. The reduction of CAII expression in GC might promote tumor cell motility and contribute to tumor growth and metastasis.

Identification of metastasis-associated genes in colorectal cancer using metaDE and survival analysis

“…Proteomics analysis identified CA2 as a potential biomarker, which exerted significant inhibitory effects on cell growth in CRC ( 23 ). CA2 expression was also associated with lymph node metastasis in gastric cancer, and a previous study reported that its reduction may contribute to tumor metastasis ( 24 ). Therefore, CA2 may also be used as a therapeutical target for metastasis.…”

Integrated bioinformatics analysis of key genes involved in progress of colon cancer

“…Carbonic anhydrase II (hCAII) has important function in physiology and pathology process. CA II highly expresses in different normal organs, but its expression is inhibited in cancer cells (Li, Xie et al, ; Sheng, Dong, Zhou, Li, & Dong, ). CA II is also associated with osteopetrosis and renal tubular acidosis (Borthwick et al, ).…”

Carbonic anhydrases II, IX, and XII in Barrett’s esophagus and adenocarcinoma

“…Downregulation of CAII was significantly associated with metastatic disease, although there was no association with survival. In gastric cancer, low CAII expression correlates with tumor size, distant metastasis, TNM-stage, and poorer overall survival [16,37]. In addition, expression of CAII has been reported significantly downregulated in metastatic gastric cancer cells [38].…”