Reduction of eotaxin production and eosinophil recruitment by pulmonary autologous macrophage transfer in a cockroach allergen-induced asthma model

Beal, Dominic R.; Stepien, David M.; Natarajan, Sudha; Kim, Jiyoun; Remick, Daniel G.

doi:10.1152/ajplung.00120.2013

Cited by 5 publications

(4 citation statements)

References 62 publications

(70 reference statements)

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“…In addition, eotaxin is the most specific and strongest factor which can affect the function of eosinophil [22]. The finding of an eotaxin decrease compared to the asthma subgroup in blood serum and BALF further showed the efficiency of γT in asthma relief.…”

Section: Discussionmentioning

confidence: 88%

Comparable Function of γ-Tocopherols in Asthma Remission by Affecting Eotaxin and IL-4

Xue

Zhang

et al. 2016

Adv Clin Exp Med

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Background. Bronchial asthma is one of the world's most common chronic disorders dangerous to human health. It has been hypothesized that the increased number of asthma sufferers may be due to changing antioxidant intake or vitamin deficiency. However, the influence of vitamins on asthma has rarely been considered. Objectives. The aim of this study was to explore the effects of γ-tocopherols, a specific form of vitamin E, on asthma remission together with the possible mechanism behind the process. Material and Methods. Eosinophil counting was applied to detect the total number of cells, eosinophils and lymphocytes. Meanwhile, HE staining was used for morphological detection. In addition, the eotaxin and IL-4 levels in the serum and bronchoalveolar lavage fluid were measured using ELISA technology. Results. The cell counting results showed that γ-tocopherols possesses the capability to reduce the number of eosinophils. Moreover, the exudation of inflammatory cells together with the hyperplasia of goblet cells was also found to experience significant inhibition when treated with γ-tocopherols. Furthermore, the high levels of eotaxin and IL-4 in the asthma group were evidently reduced under the treatment of γ-tocopherols which was comparable with hexadecadrol. Conclusions. γ-tocopherols can remit asthma by regulating the level of eotaxin and IL-4. Moreover, γ-tocopherols may be regarded as a potential candidate for asthma treatment after much deeper explorations (Adv Clin Exp Med 2016, 25, 4, 643-648).

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Section: Discussionmentioning

confidence: 88%

Comparable Function of γ-Tocopherols in Asthma Remission by Affecting Eotaxin and IL-4

Xue

Zhang

et al. 2016

Adv Clin Exp Med

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“…Alternatively, as non-pulmonary macrophages and hematopoietic macrophage precursors have been shown to acquire AM phenotypic features upon exposure to the environment of the lung [74••, 75, 76], transfer of other normal macrophages may suffice. This approach has been employed in murine models of allergic asthma, with conflicting results [77, 78]. A creative variation on this theme is exemplified by the recent report in which macrophages were initially treated ex vivo with prostaglandin E 2 (PGE 2 ) (which exerts a variety of anti-inflammatory or suppressive actions on macrophages, including strongly boosting their IL-10 production) prior to their adoptive transfer into the lungs during HDM challenge.…”

Section: Emerging Therapiesmentioning

confidence: 99%

Alveolar Macrophages in Allergic Asthma: the Forgotten Cell Awakes

Draijer

Peters‐Golden

2017

Curr Allergy Asthma Rep

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Purpose of Review The role of alveolar macrophages in innate immune responses has long been appreciated. Here, we review recent studies evaluating the participation of these cells in allergic inflammation. Recent Findings Immediately after allergen exposure, monocytes are rapidly recruited from the bloodstream and serve to promote acute inflammation. By contrast, resident alveolar macrophages play a predominantly suppressive role in an effort to restore homeostasis. As inflammation becomes established after repeated exposures, alveolar macrophages can polarize across a continuum of activation phenotypes, losing their suppressive functions and gaining pathogenic functions. Summary Future research should focus on the diverse roles of monocytes/macrophages during various types and phases of allergic inflammation. These properties could lead us to new therapeutic opportunities.

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“…Furthermore, it has been reported that IL-4 deficient mice demonstrated to be spared from asthmatic attack and airway inflammation[ 36 ]. Th2 cytokines, especially IL-4 and IL-13, are crucial mediators of asthma, and both are proved to play an important role in up-regulating the eosinophil chemo-attractants of eotaxin [ 37 ]. There is evidence showing that eosinophilia may be correlated with the induction of AHR and the severity of asthma [ 8 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

Immune-Modulatory Effects of Bu-Zhong-Yi-Qi-Tang in Ovalbumin-Induced Murine Model of Allergic Asthma

et al. 2015

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BackgroundBu-zhong-yi-qi-tang (BZYQT), an herbal formula of traditional Chinese medicine, has been an effective regimen of allergic diseases for nearly 800 years. Our previous report has demonstrated its anti-inflammatory effects in patients with perennial allergic rhinitis, and the aim of this study is to investigate the anti-asthmatic effect of BZYQT.MethodsFemale BALB/cByJNarl mice were sensitized with normal saline (control group) or OVA. Mice sensitized by OVA were fed with distilled water (OVA group), oral 0.5 g/Kg (low-dose group) or 1 g/Kg (high-dose group) of BZYQT solution once daily on days 36-40 besides their routine diet. Airway hyper-responsiveness (AHR), eosinophil infiltration, levels of cytokines and total immunoglobulin E (IgE) in broncho-alveolar lavage fluid (BALF) were determined. The lungs and tracheas were removed, and histopathologic examination was subsequently performed.ResultsAHR was significantly reduced in both low- and high-dose BZYQT groups compared with the OVA group after inhalation of the highest dose of methacholine (50 mg/ml). The levels of eotaxin, Th2-related cytokines (IL-4, IL-5, IL-13), IgE, and eosinophil infiltration in BALF were significantly decreased in both BZYQT groups compared with the OVA group. Histopathologic examination revealed that eosinophil infiltration of the lung and trachea tissues was remarkably attenuated in both BZYQT groups.ConclusionsOral administration of BZYQT solution may exert anti-asthmatic effect by relieving AHR in OVA-sensitized mice, which is compatible with our clinical experience. Although detailed mechanism is to be determined, we surmise that it may be correlated with the immune-modulatory effects of inhibiting Th2 responses on the basis of our limited results.

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Reduction of eotaxin production and eosinophil recruitment by pulmonary autologous macrophage transfer in a cockroach allergen-induced asthma model

Abstract: Beal DR, Stepien DM, Natarajan S, Kim J, Remick DG. Reduction of eotaxin production and eosinophil recruitment by pulmonary autologous macrophage transfer in a cockroach allergen-induced asthma model.

Cited by 5 publications

References 62 publications

Comparable Function of γ-Tocopherols in Asthma Remission by Affecting Eotaxin and IL-4

Comparable Function of γ-Tocopherols in Asthma Remission by Affecting Eotaxin and IL-4

Alveolar Macrophages in Allergic Asthma: the Forgotten Cell Awakes

Immune-Modulatory Effects of Bu-Zhong-Yi-Qi-Tang in Ovalbumin-Induced Murine Model of Allergic Asthma

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