Reduction of Escherichia coli O157:H7 viability on hard surfaces by treatment with a bacteriophage mixture

Viazis, Stelios; Akhtar, Mastura; Fęirtag, Joellen M.; Diez-Gonzalez, Francisco

doi:10.1016/j.ijfoodmicro.2010.11.021

Cited by 79 publications

(46 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Recently the use of bacteriophages has been investigated as a new tool to control pathogenic microorganisms. [49][50][51][52] A mixture of bacteriophages was used to inactivate EHEC dependent on increasing values of incubation time, temperature, and multiplicity of infection units. 49 At temperatures above 12°C there was more than a 3 log 10 CFU reduction within 10 min of applying bacteriophages on EHEC.…”

Section: Photochemical and Photobiological Sciences Papermentioning

confidence: 99%

Dirty hands: photodynamic killing of human pathogens like EHEC, MRSA and Candida within seconds

Eichner

Gonzales

Felgenträger

et al. 2012

Photochem Photobiol Sci

View full text Add to dashboard Cite

aHand hygiene is one of the most important interventions for reducing transmission of nosocomial lifethreatening microorganisms, like methicillin resistant Staphylococcus aureus (MRSA), enterohemorrhagic Escherichia coli (EHEC) or Candida albicans. All three pathogens have become a leading cause of infections in hospitals. Especially EHEC is causing severe diarrhoea and, in a small percentage of cases, haemolytic-uremic syndrome (HUS) as reported for E. coli 104:H4 in Germany 2011. We revealed the possibility to inactivate very fast and efficiently MRSA, EHEC and C. albicans using the photodynamic approach.MRSA, EHEC and C. albicans were incubated in vitro with different concentrations of TMPyP for 10 s and illuminated with visible light (50 mW cm TMPyP on ex vivo porcine skin, fluorescence microscopy of histology showed that the photosensitizer was exclusively localized in the stratum corneum regardless of the incubation time. TMPyP exhibited a fast and very effective killing rate of life-threatening pathogens within a couple of seconds that encourages further testing in an in vivo setting. Being fast and effective, antimicrobial photodynamic applications might become acceptable as a tool for hand hygiene procedures and also in other skin areas.

show abstract

Section: Photochemical and Photobiological Sciences Papermentioning

confidence: 99%

Dirty hands: photodynamic killing of human pathogens like EHEC, MRSA and Candida within seconds

Eichner

Gonzales

Felgenträger

et al. 2012

Photochem Photobiol Sci

View full text Add to dashboard Cite

show abstract

“…37°C ve 12°C'de paslanmaz çelik yüzeylerde 10 dakika gibi k›sa bir süre içerisinde patojen varl›¤› tespit edilemezken, 23°C'de 1 saatin sonunda ayn› sonuca ulafl›lm›flt›r. Seramik yüzeylerde ise, 37°C'de 10 dakika, 23°C'de 1 saatten sonra benzer sonuç elde edilmifltir (62). Söz konusu çal›flma kontamine sert yüzeylerde faj uygulamas›n›n etkinli¤ini destekler nitelikte sonuçlar vererek bakteriyofajlar›n biyosanitasyon ajan› olarak kullan›m›n›n önemini vurgulayan di¤er çal›flmalar› da destekler niteliktedir (63).…”

Section: Bakteriyofaj Enkapsülasyonu Ve Potansiyel Uygulamalarıunclassified

Bakteri̇yofaj Enkapsülasyonu Ve Potansi̇yel Uygulamalari

Saygılı¹,

Karagözlü²

2017

GIDA

View full text Add to dashboard Cite

ÖzetEnkapsülasyon, aktif olan maddenin çevresinde uygun kaplama materyali ile koruyucu bir zar oluflturulmas› temeline dayanmaktad›r. G›da endüstrisinde mikroenkapsülasyon istenen bileflenin d›fl etkenlere karfl› korunmas›, baz› özelliklerinin sürdürülebilmesi, kolay tafl›nmas›, tat ve koku maskeleme, oluflabilecek reaksiyonlar›n önüne geçme gibi amaçlarla kullan›lmas›na ra¤men koruyucu ve tedavi amaçl› fajlar›n enkapsülasyonunun da oldukça etkili bir yöntem olarak uygulanabildi¤i bilinmektedir. Bakteriyofajlar 20. yüzy›l›n bafllar›ndan beri bakteriyel enfeksiyonlara karfl› koruma ve tedavi amac›yla kullan›lm›fl ancak artan antibiyotik tüketimi, fajlar›n tedavi amaçl› kullan›m›n›n önüne geçmifltir. En önemli özelli¤i yüksek spesifitesi olan bakteriyofajlar, çiftlik hayvanlar›nda patojen kolonizasyonunun azalt›lmas› (faj tedavisi), çi¤ süt, et ve taze g›dalarda dekontaminasyon (biyokontrol), ekipman yüzeylerinde sanitasyon (biyosanitasyon), haz›r g›dalarda raf ömrü uzatmak (biyoprezervasyon) amac›yla uygulanabilen etkin bir yöntem olarak karfl›m›za ç›kmaktad›r. Bakteriyofaj enkapsülasyon uygulamalar›nda, fonksiyonel kaplama uygulamalar›ndaki geliflmelere paralel olarak daha uygun maliyetli ve daha uzun süre muhafaza edilebilen mikrokapsül teknolojilerinin gelifltirilmesine ihtiyaç duyulmaktad›r.Anahtar kelimeler: Enkapsülasyon, bakteriyofaj, faj tedavi, biyokontrol, biyosanitasyon, biyoprezervasyon BACTERIOPHAGE ENCAPSULATION AND POTENTIAL APPLICATIONS AbstractEncapsulation is based on forming a protective membrane around the active substance with a suitable coating material. Although microencapsulation is applied for purposes including protection of some components against external factors, preservation of some features, easy handling, flavor and odor masking, prevention of some reactions, it is known that the phage encapsulation can be highly effective for protection and treatment purposes. Since the early 20th century, bacteriophages are used for the prevention and treatment of bacterial infections, however the increase in the consumption of antibiotics has prevented the use of phages for treatment. One of the most important properties of bacteriophages is the high specificity. They are effectively used for the reduction of pathogen colonization in farm animals (phage therapy), decontamination of raw milk, meat and fresh food (biocontrol), sanitation of equipment surfaces (biosanitation), extending the shelf life of prepared foods (biopreservation). There is a need for the development of microcapsule technology which is relatively cost effective and allows longer storage periods in parallel with the developments in functional coating applications.

show abstract

“…Carvalho et al (2010) administered campylobacter phage to poultry by oral gavage Spraying of phage cocktail on spinach blades resulted in a 4.5 log reduction of CFU after 2 h of phage addition Patel et al 2011 No survivors detectable on spinach and lettuce leaves after 10 min. of phage addition combined with cinnamaldehyde treatment Viazis et al 2011 Significant reduction in CFU on lettuce and cantaloupe after 2 days of spraying with phage cocktail (ECP-100) Sharma et al 2009 Phage cocktail e11/2, e4/1c, pp01 applied on meat surface resulted in eradication of E.coli in seven of nine samples…”

Section: Phage Therapymentioning

confidence: 99%

“…In another study Montanez-Izquierdo et al (2012) evaluated Listeria phage P100 to control biofilm formation by L. monocytogenes on stainless steel surfaces and found a mean reduction of 5.29 log CFU/cm 2 . Apart from controlling Listeria biofilms, Campylobacter biofilms were successfully removed from the surface of glass (Siringan et al 2011) and growth of E. coli O157:H7 was controlled using phage mixture BEC8 on stainless steel and ceramic tiles (Viazis et al 2011). Use of phage for biosanitation is promising although very challenging due to the diversity of bacteria found in different environments (Sillankorva et al 2012).…”

Section: Biosanitationmentioning

confidence: 99%

Bacteriophage biocontrol of foodborne pathogens

2015

View full text Add to dashboard Cite

Bacteriophages are viruses that only infect bacterial cells. Phages are categorized based on the type of their life cycle, the lytic cycle cause lysis of the bacterium with the release of multiple phage particles where as in lysogenic phase the phage DNA is incorporated into the bacterial genome. Lysogeny does not result in lysis of the host. Lytic phages have several potential applications in the food industry as biocontrol agents, biopreservatives and as tools for detecting pathogens. They have also been proposed as alternatives to antibiotics in animal health. Two unique features of phage relevant for food safety are that they are harmless to mammalian cells and high host specificity, keeping the natural microbiota undisturbed. However, the recent approval of bacteriophages as food additives has opened the discussion about 'edible viruses'. This article reviews in detail the application of phages for the control of foodborne pathogens in a process known as Bbiocontrol^.

show abstract

Reduction of Escherichia coli O157:H7 viability on hard surfaces by treatment with a bacteriophage mixture

Cited by 79 publications

References 34 publications

Dirty hands: photodynamic killing of human pathogens like EHEC, MRSA and Candida within seconds

Dirty hands: photodynamic killing of human pathogens like EHEC, MRSA and Candida within seconds

Bakteri̇yofaj Enkapsülasyonu Ve Potansi̇yel Uygulamalari

Bacteriophage biocontrol of foodborne pathogens

Contact Info

Product

Resources

About