Reduction of ethylene biosynthesis in sugarcane induces growth and investment in the non-enzymatic antioxidant apparatus

Neris, Daniel Moreira; Mattiello, Lúcia; Zúñiga, Gustavo E.; Purgatto, Eduardo; Menossi, Marcelo

doi:10.1007/s00299-022-02832-7

Cited by 4 publications

(1 citation statement)

References 85 publications

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“…Primers for ScGUX2 were used in qPCR reactions to evaluate gene silencing using endogenous polyubiquitin gene as control for normalization (UBI; SUCEST clone: SCCCST2001G02.g; NCBI accession CA179923.1) as described before (Neris et al ., 2022 ). The transcription levels of ScGUX2 in silenced plants were compared to wild‐type plants, in different tissues: development culm (third internode), mature culm (seventh internode) and leaves.…”

Section: Methodsmentioning

confidence: 99%

Silencing ScGUX2 reduces xylan glucuronidation and improves biomass saccharification in sugarcane

Gallinari,

Lyczakowski,

Llerena

et al. 2023

Plant Biotechnology Journal

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SummaryThere is an increasing need for renewable energy sources to replace part of our fossil fuel‐based economy and reduce greenhouse gas emission. Sugarcane bagasse is a prominent feedstock to produce cellulosic bioethanol, but strategies are still needed to improve the cost‐effective exploitation of this potential energy source. In model plants, it has been shown that GUX genes are involved in cell wall hemicellulose decoration, adding glucuronic acid substitutions on the xylan backbone. Mutation of GUX genes increases enzyme access to cell wall polysaccharides, reducing biomass recalcitrance in Arabidopsis thaliana. Here, we characterized the sugarcane GUX genes and silenced GUX2 in commercial hybrid sugarcane. The transgenic lines had no penalty in development under greenhouse conditions. The sugarcane GUX1 and GUX2 enzymes generated different patterns of xylan glucuronidation, suggesting they may differently influence the molecular interaction of xylan with cellulose and lignin. Studies using biomass without chemical or steam pretreatment showed that the cell wall polysaccharides, particularly xylan, were less recalcitrant in sugarcane with GUX2 silenced than in WT plants. Our findings suggest that manipulation of GUX in sugarcane can reduce the costs of second‐generation ethanol production and enhance the contribution of biofuels to lowering the emission of greenhouse gases.

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Section: Methodsmentioning

confidence: 99%

Silencing ScGUX2 reduces xylan glucuronidation and improves biomass saccharification in sugarcane

Gallinari,

Lyczakowski,

Llerena

et al. 2023

Plant Biotechnology Journal

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Proteomic investigation reveals molecular mechanisms of plant height regulation in foxtail millet

Zhao,

Li,

Wang

et al. 2024

Journal of Integrative Agriculture

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Harnessing Genetic Tools for Sustainable Bioenergy: A Review of Sugarcane Biotechnology in Biofuel Production

Ahmad,

Ming

2024

Agriculture

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Sugarcane (Saccharum spp.) is a prominent renewable biomass source valued for its potential in sustainable and efficient second-generation biofuel production. This review aims to assess the genetic enhancement potential of sugarcane, emphasizing the use of advanced genetic engineering tools, such as CRISPR-Cas9, to improve traits crucial for biomass yield and biofuel production. The methodology of this review involved a thorough analysis of the recent literature, focusing on the advancements in genetic engineering and biotechnological applications pertinent to sugarcane. The findings reveal that CRISPR-Cas9 technology is particularly effective in enhancing the genetic traits of sugarcane, which are essential for biofuel production. Implementing these genomic tools has shown a significant rise in biomass output and, ultimately, the effectiveness of bioethanol manufacturing, establishing sugarcane as a feasible and reliable source of biofuel implications of these advancements extend. These advancements have a profound impact not only on agricultural productivity but also on enhancing the efficiency and scalability of the bioethanol industry. Developing superior sugarcane varieties is expected to boost economic returns and advance environmental sustainability through carbon-neutral biofuel alternatives. This review underscores the transformative role of genetic engineering in revolutionizing sugarcane as a bioenergy crop. The evolution of genetic engineering tools and methodologies is crucial for tapping into the full potential of sugarcane, and thereby supporting global efforts towards sustainable energy solutions. Future research should focus on refining these biotechnological tools to meet increasing energy demands sustainably, ensure food security, and mitigate negative environmental impacts.

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Reduction of ethylene biosynthesis in sugarcane induces growth and investment in the non-enzymatic antioxidant apparatus

Cited by 4 publications

References 85 publications

Silencing ScGUX2 reduces xylan glucuronidation and improves biomass saccharification in sugarcane

Silencing ScGUX2 reduces xylan glucuronidation and improves biomass saccharification in sugarcane

Proteomic investigation reveals molecular mechanisms of plant height regulation in foxtail millet

Harnessing Genetic Tools for Sustainable Bioenergy: A Review of Sugarcane Biotechnology in Biofuel Production

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