Reduction of extended-spectrum-β-lactamase- and AmpC-β-lactamase-producing Escherichia coli through processing in two broiler chicken slaughterhouses

Pacholewicz, Ewa; Liakopoulos, Apostolos; Swart, Arno; Gortemaker, Betty G. M.; Dierikx, Cindy; Havelaar, Arie H.; Schmitt, Heike

doi:10.1016/j.ijfoodmicro.2015.08.010

Cited by 39 publications

(45 citation statements)

References 36 publications

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“…Additionally, we noted that ESBL/AmpC production was detected in only two of the 142 (1Á4%) E. coli characterized in the present study. This frequency was much lower than those recently reported for isolates from pig faeces (20%) in Korea and for isolates from broiler chicken (82%) at slaughterhouses in Germany and the Netherlands (Pacholewicz et al 2015;Han et al 2016).…”

Section: Discussioncontrasting

confidence: 73%

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Comparison by multilocus variable-number tandem repeat analysis and antimicrobial resistance among atypical enteropathogenicEscherichia colistrains isolated from food samples and human and animal faecal specimens

et al. 2016

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Aim: This study assessed whether multilocus variable-number tandem repeat analysis (MLVA) and antimicrobial susceptibility testing discriminated diarrhoeagenic atypical enteropathogenic Escherichia coli (aEPEC) from aEPEC indigenous to domestic animals or healthy people. Methods and Results: MLVA genotyping of 142 aEPEC strains isolated from foods and faecal samples of domestic animals and humans revealed 126 distinct MLVA profiles that distributed to four clusters, yielding a Simpson's index of diversity (D) of 99Á8%. Cluster 2 included 87% of cattle isolates and 67% of patient isolates. The plurality (15/34, 44%) of strains from healthy humans mapped to Cluster 1, while half (18/41, 44%) of the swine strains belonged to Cluster 4. Testing for antimicrobial susceptibility revealed that 52 strains (37%) of aEPEC were resistant to one or more agents; only 10 strains (7%) exhibited resistance to more than three agents. Strains isolated from swine or food exhibited a wider variety of resistance phenotypes than bovine or human strains. Conclusions: MLVA assigned the aEPEC isolates from cattle and patients to Cluster 2, distinct from aEPEC from other sources. Hog yards may be a larger source of drug-resistant strains than are cattle ranches. Significance and Impact of the Study: MLVA suggests that human diarrhoeagenic aEPEC are derived from cattle and are distinct from strains carried by healthy people and other animals. Cattle appear to be reservoirs of human diarrhoeagenic aEPEC.

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Section: Discussioncontrasting

confidence: 73%

“…This frequency was much lower than those recently reported for isolates from pig faeces (20%) in Korea and for isolates from broiler chicken (82%) at slaughterhouses in Germany and the Netherlands (Pacholewicz et al . ; Han et al . ).…”

Section: Discussionmentioning

confidence: 99%

Comparison by multilocus variable-number tandem repeat analysis and antimicrobial resistance among atypical enteropathogenicEscherichia colistrains isolated from food samples and human and animal faecal specimens

et al. 2016

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“…Resistances to TET, AMP, and SXT among DEC strains isolated from food samples are also common in other reports (Canizalez-Roman et al, 2013;Teophilo et al, 2002). Despite the high isolation rates in chicken (Abdallah et al, 2015;Pacholewicz et al, 2015), no ESBL producing E. coli was found in this study. However, EAST1EC showed a high multi-drug resistant rate of 31% (more than 3 antimicrobials) including 2 strains of AmpC-producing EAST1EC.…”

Section: Antimicrobial Susceptibility Testing Of Isolates By the Discsupporting

confidence: 46%

Prevalence, antimicrobial resistance and multiple-locus variable-number tandem-repeat analysis profiles of diarrheagenic Escherichia coli isolated from different retail foods

Wang

Nakamura

Kage-Nakadai

et al. 2017

International Journal of Food Microbiology

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Diarrheagenic E. coli (DEC) isolates were recovered from local retail markets and the Osaka Municipal Central Wholesale Market in Japan. Retail food samples were collected for analysis in Osaka Japan from 2005 to 2008 and consisted of 32 beef, 28 pork, 20 poultry, 136 fish, 66 fruits and vegetables and 51 ready-to-eat (RTE) food samples. A total of 82 DEC strains were recovered from 64 (19%) food samples with the highest prevalence in poultry (100%, 20/20), followed by pork (54%, 15/28), beef (28%, 9/32), fruits and vegetables (12%, 8/66), fish (6.6%, 9/136) and RTE foods (5.9%, 3/51). Most of the strains belonged to E. coli possessing the enteroaggregative E. coli (EAEC) heat-stable enterotoxin 1 (EAST1) gene (EAST1EC; n=62, P<0.0001) and enteropathogenic E. coli (EPEC; n=16, P<0.01), whereas only 1 strain belonged to Shiga toxin-producing E. coli (STEC), 1 to EAEC and 2 to enterotoxigenic E. coli (ETEC) strains. Of the 82 DEC isolates, 22 O and 13H serogroups were detected, including some specific serogroups (O91, O103, O115, O119, O126, and O157) which have been associated with human diarrheal infections. Phylogenetic group A and B1 were predominant among the DEC isolates. Antimicrobial resistance to tetracycline was most common (49%), followed by nalidixic acid (28%), ampicillin (24%), sulfamethoxazole/trimethoprim (20%), and cephalothin (18%). All isolates were susceptible to aztreonam. Of the resistant strains, 44% (22/50) demonstrated resistance to >3 antimicrobial agents. Isolates resistant to >5 antimicrobials were only found in the meat samples, while isolates from the fruits and vegetables as well as RTE foods showed resistance to only 1 or 2 antimicrobial agents. Sixty one percent of EAST1EC, 56% of EPEC and all of the EAEC and ETEC were resistant to at least 1 antimicrobial agent. Multiple-locus variable-number tandem repeat analysis (MLVA) was used in this study for genotyping of DEC. The 82 isolates collected for this study showed 77 distinct MLVA profiles located among 3 branches. The Simpson's Index of Diversity (D) was 99.9% at its highest. The high diversity of these food strains would suggest their originating from a variety of sources and environments. In conclusion, retail food samples in Japan were contaminated with DEC; EAST1EC, a putative DEC, were detected at high rates in poultry, pork and beef. Isolates resistant to >3 antimicrobials were found only in raw meat and fish. Food animals may act as the reservoir for multi-resistant bacteria. Due to the finding that nearly 1/3 of EAST1EC strains were resistant to >3 antimicrobials, additional surveillance for EAST1EC should be initiated.

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“…However, ampC β-lactamases provide resistance to penicillins, 2 nd and 3 rd -generation cephalosporins, including β-lactam/inhibitor combinations, cefamycins (cefoxitin), but usually not to 4 th -generation cephalosporins (cefepime, cefquinome) and carbapenems (eFSa PaNeL ON BIOLOGICaL haZarDS, 2011) The economic burden of antimicrobial resistance is significant, due to delayed response to treatment and prolonged stay in hospital (De kraker et al, 2011). there are three classical eSBLs, i.e. teM (except teM-1), ShV (except ShV-1 and 2) and CtX-M (eFSa PaNeL ON BIOLOGICaL haZarDS, 2011). among food animals that act as reservoirs of eSBL-producing E. coli, broilers are considered to be the most potent reservoir (PaChOLeWICZ et al, 2015). almost all the eSBL genes are carried by mobile genetic elements of E. coli, and the genes can spread both clonally and horizontally among different lineages of E. coli (GhODOUSI et al, 2015).…”

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confidence: 99%

Occurrence of extended-spectrum cephalosporinase producing Escherichia coli in kuroiler birds

et al. 2017

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the present study was undertaken to detect the incidence of extended-spectrum-cephalosporinase gene possessing-Escherichia coli, its co-resistance pattern against other antimicrobials, and the clonal relationship of the isolates in healthy kuroiler birds. a total of 80 cloacal swabs from kuroilers were collected randomly from West Bengal, India. the use of costly antimicrobials (cephalosporins) was not practiced by farmers. Escherichia coli was isolated and identified by standard biochemical tests and 16SrRNA-PCR. All the E. coli isolates, including controls, were subjected to PCr for detection of bla CTX-M , bla TEM, bla SHV, and bla CMY-2 genes. By comparing the raPD-banding pattern, the phylogenetic relationship among the isolates was established. all the isolates were tested for phenotypical resistance against other antibiotics. In total, 60 isolates were identified as E. coli from the kuroilers studied (n = 80). among them, 12 (20%) isolates possessed one of the studied extended-spectrum cephalosporinase genes. among the studied genes, bla TEM and bla SHV were detected in 6 (10%) and 12 (20%) E. coli isolates, respectively. None of the E. coli isolates possessed bla CTX-M and bla In phylogenetic analysis, the strains isolated from same localities with similar genetic profile were grouped into the same cluster. resistance of extended-spectrum cephalosporinase gene possessing E. coli isolates was observed most frequently against ampicillin/cloxacillin, co-trimoxazole, amoxyclav, piperacillin, ceftriaxone, and tetracycline. Kuroiler birds with no cephalosporin usage profile may act as a reservoir of extended-spectrum cephalosporinase gene possessing E. coli. This is the first systematic study in kuroilers, to raise the awareness of consumers regarding the possibility of transmission of antimicrobial resistant E. coli from them.

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Reduction of extended-spectrum-β-lactamase- and AmpC-β-lactamase-producing Escherichia coli through processing in two broiler chicken slaughterhouses

Cited by 39 publications

References 36 publications

Comparison by multilocus variable-number tandem repeat analysis and antimicrobial resistance among atypical enteropathogenicEscherichia colistrains isolated from food samples and human and animal faecal specimens

Comparison by multilocus variable-number tandem repeat analysis and antimicrobial resistance among atypical enteropathogenicEscherichia colistrains isolated from food samples and human and animal faecal specimens

Prevalence, antimicrobial resistance and multiple-locus variable-number tandem-repeat analysis profiles of diarrheagenic Escherichia coli isolated from different retail foods

Occurrence of extended-spectrum cephalosporinase producing Escherichia coli in kuroiler birds

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