Orthogonal frequency division multiple access (OFDMA) is a multiuser of digital modulation technique used to increase wireless transmission rate. Signal efficiency is distorted due to Inter symbol interference (ISI) that occurs due to the multipath propagation. Cascaded Least Square (CLS) with Decision Feedback Equalizer (DFE) had been used in this research in order to mitigate ISI in OFDMA system. CLS is combination of two adaptive algorithms which are Least Mean Square (LMS) and Recursive Least Square (RLS). This research also justified that combination of space time frequency block code (STFBC) with multiple input multiple output (MIMO) antenna enable to minimize ISI. The simulation results show the improvement of bit error rate (BER) performance after reducing ISI and also prove that STFBC was able to achieve maximum diversity order in the MIMO OFDMA system. Index Terms-Least mean square (LMS), recursive least square (RLS), cascaded least square (CLS), decision feedback equalizer (DFE). Siti Maisurah Sulong received her B.Eng. (Hon.) degree in communication from Universiti Teknologi MARA (UiTM), Shah Alam in 2014. She currently pursues her M.Sc. degree (electrical) in UiTM. Her research interests include wireless telecommunication, OFDMA for the WiMax, adaptive algorithm, equalizers and diversity scheme. Azlina Idris received her B.Eng. (Hon.) degree from Leeds Metropolitan University and M.Sc. degree from Universiti Teknologi Malaysia. She completed her PhD degree in Universiti Malaya and now holds position as a senior lecturer in UiTM Shah Alam. Besides that, she is also the coordinator for student employability. Her research interests include pair wise error probability in OFDM, diversity scheme, wireless interference and equalizers. Suzi Seroja Sarnin received her B.Eng. (Hon) degree from Universiti Teknologi Malaysia (UTM), Malaysia in 1999 and M.Sc. (microelectronic), degree from Universiti Kebangsaan Malaysia (UKM), Malaysia in 2006 respectively. She currently pursues her PhD degree in UiTM. Her research interests include channel estimation, coding algorithm for CDMA and WCDMA, mobile telecommunication, synchronization algorithm in OFDM for the Wimax technology application.