Reduction of Laccase Activity in dsRNA-Containing Hypovirulent Strains of<i>Cryphonectria (Endothia) parasitica</i>

Rigling, Daniel; Heiniger, U.; Hohl, Hans R.

doi:10.1094/phyto-79-219

Cited by 134 publications

(108 citation statements)

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“…Small insects and large insects, which were cut into small pieces, were placed on a medium containing a mixture of 0.5% tannic acid (Sigma-Aldrich, St. Louis, MO, USA), 1.5% Difco malt extract, and 2% Difco Bacto agar (Rigling et al, 1989;Daniel Rigling, personal communication). To assess culture characteristics of the isolates, all C. parasitica isolates were grown on PDA plates at 25 °C for 12 h in the dark and 12 h in the light for 7 days.…”

Section: Collection Of Bark and Invertebrate Samples From Healed Cankersmentioning

confidence: 99%

Natural dissemination of hypovirulent Cryphonectria parasiticastrain used for biological control of chestnut blight

Çelİker¹,

Kaplan²,

Onoğur³

et al. 2017

Turk J Agric For

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Section: Collection Of Bark and Invertebrate Samples From Healed Cankersmentioning

confidence: 99%

Natural dissemination of hypovirulent Cryphonectria parasiticastrain used for biological control of chestnut blight

Çelİker¹,

Kaplan²,

Onoğur³

et al. 2017

Turk J Agric For

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“…Extraction and detection of dsRNA was performed in selected C. parasitica isolates, that were phenotypically considered hypovirulent, according to the method of Morris and Dodds (1979) modified by Rigling et al (1989) with some minor adaptations. Double stand of RNA was extracted from C. parasitica mycelium grown on cellophanecovered PDAmb.…”

Section: Methodsmentioning

confidence: 99%

Potential of Local Hypovirulent Strains of Cryphonectria Parasitica for Biological Control of Chestnut Blight

Gouveia¹,

Coelho²,

Monteiro³

2010

Acta Hortic.

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Cryphonectria parasitica, introduced in the NE of Portugal since 1989, is now an important and widespread pathogen of chestnut (Castanea sativa Mill.) which causes losses in chestnut fruit production and led to decline and death of many chestnut trees. Hypovirulence, a virus mediated attenuation of fungal pathogenesis, has not been frequent until now in Portugal but recently some cases of healing cankers were reported by chestnut growers. We assessed these sites and one of them, where healing cankers were clustered, was intensively surveyed. From all the trees with healing cankers, white and orange phenotypes of C. parasitica were isolated, which are both of the same vc type (EU-11). White isolates of C. parasitica showed reduced pigmentation in colony colour which was not completely stable, suppressed conidiation, reduction of phenol oxidase but no significant differences in lesion length and sporulation on chestnut twigs on dormant chestnut trees. Hypovirulent strains have dsRNA virus in the cytoplasm which is the typical element that reduces virulence in C. parasitica. Bands of dsRNA were identified in all of the white isolates and some variation in band dimension and number of bands was detected. The LdsRNA (12 kbp), which is the diagnostic fragment of C. parasitica infection, was detected. The presence of white phenotypes of infected C. parasitica (CHV) and the fact that spreads occur in natural conditions is promising for its development as a biological control agent of chestnut blight.

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“…Hongos infectados sólo son capaces de formar superficial (curación) cancros en los tallos, y por lo tanto los árboles pueden sobrevivir a la enfermedad. Otros síntomas de la presencia de CHV-1 en los aislados incluyen cambios en su morfología y color (Peever et al, 1998;Rigling, Heiniger, & Hohl, 1989). También es conocido el sobre el control de otros patógenos como Ophiostoma novo-ulmi en el que también el micovirus genera un crecimiento micelial lento, anormal, formaciones coloniales amorfas, reducción en la producción de esporas asexuales, bajo citocromo a nivel oxidasa y formación de plásmidos de ADN mitocondrial (Hong, Dover, Cole, Brasier, & Buck, 1999).…”

Section: Uso De Micovirusunclassified

Chancro Resinoso Del Pino (Fusarium Circinatum) Historia, Evolución, Dispersión Y Estrategias De Manejo

Flores-Pacheco¹

2017

Nexo Revista Científica

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RESUMENLa enfermedad denominada con el nombre de Chancro Resinoso del Pino es causada por el hongo ascomicete Fusarium circinatum Nirenberg et O'Donnell (teleomorfo= Gibberella circinata). Afecta numerosas especies de coníferas y a la fecha tiene presencia en todos los continentes del mundo, a excepción de la Antártida. Debido a las variaciones climatológicas y ambientales a nivel global que han favorecido el rango biológico de dispersión de la enfermedad, sumado al comercio internacional de madera y sus derivados con medidas cuarentenarias insuficientes, actualmente el hongo amenaza con expandirse a zonas libres de la infección. En las zonas donde ya se ha confirmado la presencia de la enfermedad se reportan importantes pérdidas económicas en viveros, semilleros forestales, plantaciones silvestres y comerciales de distintas especies de coníferas. No menos importante pérdida ecosistémica causada por la inestabilidad de los bosques y nichos ecológicos altamente sensibles a las variaciones florísticas. Actualmente es considerada la patología más importante a nivel mundial de las coníferas, tanto silvestres como comerciales, y no posee tratamiento curativo por lo que los esfuerzos están centrados en el tratamiento preventivo. Dentro del ámbito científico destacan las investigaciones en resistencia genética del hospedante y el uso de controladores biológicos que prometan resultados significativos.Palabras claves: Sanidad forestal; Biocontrol; Selección y Mejora genética; Epidemiología; Fisiología del estrés en plantas.

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Reduction of Laccase Activity in dsRNA-Containing Hypovirulent Strains ofCryphonectria (Endothia) parasitica

Cited by 134 publications

References 0 publications

Natural dissemination of hypovirulent Cryphonectria parasiticastrain used for biological control of chestnut blight

Natural dissemination of hypovirulent Cryphonectria parasiticastrain used for biological control of chestnut blight

Potential of Local Hypovirulent Strains of Cryphonectria Parasitica for Biological Control of Chestnut Blight

Chancro Resinoso Del Pino (Fusarium Circinatum) Historia, Evolución, Dispersión Y Estrategias De Manejo

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