Reduction of Phytophthora Capsici Oospores Viability by Gases Released During Soil Biosolarization of Protected Pepper Crops in Southeastern Spain

Larregla, Santiago; Núñez-Zofío, M.; Fernández-Molina, P.; Martinez-Alarcon, V.; Lacasa-Martinez, C.M.; Guerrero-Diaz, M. M.

doi:10.17660/actahortic.2014.1044.12

Cited by 2 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The low disinfectant efficacy of biodisinfestation gases on oospore viability observed in this study in northern Spain (Biscay) contrasted with the higher efficacy of a previous experiment carried out with the same methodology in southeastern Spain (Murcia) (Larregla et al, 2014). The differences in efficacy between both studies seemed to be closely related to the different applied doses of organic amendment (70,000 kg ha −1 of fresh sheep manure in southeastern Spain vs. 25,630 kg ha −1 in northern Spain) and the 15 cm depth biodisinfested soil temperatures (average daily minimummaximum values of 37.7-42.1 • C in southeastern Spain vs. 29.2-36.8 • C in northern Spain).…”

Section: Discussioncontrasting

confidence: 90%

“…This treatment showed a viability reduction rate of 41% compared with the untreated control for an exposure time of 21 days. Similarly, the higher reduction (72% for an exposure time of 34 days) was also obtained for the same treatment in the previous study in southeastern Spain (Larregla et al, 2014). The following more effective biodisinfestation gas treatments were similar in both studies, since they were detected between days 4 and 11 in the present study in northern Spain (reduction of 24-34%) and between days 3 and 9 (reduction of 17-28%) in the previous study in southeastern Spain.…”

Section: Discussionsupporting

confidence: 86%

“…This explanation would be consistent with the result that the biodisinfested soil control (SOIL) was the only treatment that improved disinfectant efficacy when exposure time increased. It also confirms our previous findings where the succession of gases sampled every day (G_ev) was the most effective treatment (Larregla et al, 2014). In order to avoid gas exhaustion and negligible effects on survival by low gas concentrations, the use of methodological devices (Klein et al, 2007) which allow the exposition of fungal spores to larger volumes of gas samples are recommended in future experiments.…”

Section: Discussionsupporting

confidence: 85%

“…Both factors could have ultimately resulted in the generation of a small quantity of biotoxic volatile compounds and therefore, in a low impact on the survival of P. capsici oospores, as previously reported with various Phytophthora spp. survival spores (Guerrero et al, 2010;Larregla et al, 2014;Lacasa et al, 2015;Gandariasbeitia et al, 2019) or with other soilborne fungal pathogens survival structures (Gamliel et al, 2000;Stapleton, 2000, Gamliel andStapleton, 2017).…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Gases Released During Soil Biodisinfestation of Pepper Greenhouses Reduce Survival of Phytophthora capsici Oospores in Northern Spain

Larregla

Gandariasbeitia

Ojinaga

et al. 2021

Front. Sustain. Food Syst.

View full text Add to dashboard Cite

Phytophthora capsici is one of the oomycetes that affects protected pepper crops in different agroclimatic areas of Spain. Currently, environmentally friendly strategies such as biodisinfestation for plant disease control have become increasingly popular. In this study, the effect of released gases during biodisinfestation with a fresh manures mixture amendment on P. capsici oospore viability was determined. A biodisinfestation trial was performed in a greenhouse located in northern Spain (Biscay), with a mixture of fresh sheep (2 kg m−2) and dry poultry manures (0.5 kg m−2) followed by soil sealing with a transparent polyethylene plastic film for 21 days (onset June 15th). Gases were sampled from the aerial cavity of biodisinfested plots at different days after soil sealing (0–1–2–3–4–7–9–11, and 14 days). Vacutainer tubes were incubated at 20°C with oospores of P. capsici that were previously placed under vacuum and refilled with extracted gases. Treatments assayed were gases from different sampling times (0–1–2–3–4–7–9–11–14 days, and succession of days 1–2–3–4–7–9–11–14) combined with different exposure times (7–14–21 days) at 20°C in the laboratory. Control treatments were included: air-tubes and vacuum-tubes. An additional reference treatment under real field conditions was also considered: buried oospores at 15 cm depth in the biodisinfested plots. Oospore viability was determined with the plasmolysis method. The most effective treatment was the succession of gases collected during all sampling days. The significant but slight reduction in oospore viability by exposure to the different gas treatments was consistent with the low dose of applied amendment and the low soil temperature registered at 15 cm depth during soil biodisinfestation (>25°C−100% time, >35°C−23%, >40°C−3%). The above circumstances might have generated a small quantity of gases with low impact on oospore viability. The biodisinfested soil at 15 cm depth reference treatment showed the lowest oospore viability in all the exposure times assayed. The overlap of thermal and higher biofumigation effects in this treatment could likely be responsible for its greater efficacy. A disinfectant effect purely attributable to released gases throughout biodisinfestation has been demonstrated. We believe that our research will serve as a base for future application in agro-environments with reduced thermal inactivation effects.

show abstract

Section: Discussioncontrasting

confidence: 90%

Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Gases Released During Soil Biodisinfestation of Pepper Greenhouses Reduce Survival of Phytophthora capsici Oospores in Northern Spain

Larregla

Gandariasbeitia

Ojinaga

et al. 2021

Front. Sustain. Food Syst.

View full text Add to dashboard Cite

show abstract

“…Furthermore, the ability of Phytophthora to overcome the genetic resistance of plants owing to its genetic variability creates the need for alternative control methods for both diseases. Different cultivation techniques, such as grafting on resistant rootstocks [ 30 , 31 , 32 , 33 , 34 ] or nonchemical disinfection methods [ 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 ], have been used as alternatives. Crop rotation is a key component in the integrated management of diseases caused by Phytophthora ; nevertheless, the survival of oospores, even in the absence of hosts, limits the effectiveness of these methods [ 21 , 44 ].…”

Section: Introductionmentioning

confidence: 99%

Biocontrol of Diseases Caused by Phytophthora capsici and P. parasitica in Pepper Plants

Santos

Diánez

Sánchez-Montesinos

et al. 2023

JoF

View full text Add to dashboard Cite

The main objective of this study was to evaluate the ability of Trichoderma aggressivum f. europaeum, T. longibrachiatum, Paecilomyces variotii, and T. saturnisporum as biological control agents (BCAs) against diseases caused by P. capsici and P. parasitica in pepper. For this purpose, their antagonistic activities were evaluated both in vitro and in vivo. We analysed the expression patterns of five defence related genes, CaBGLU, CaRGA1, CaBPR1, CaPTI1, and CaSAR8.2, in leaves. All BCAs showed a high in vitro antagonistic activity, significantly reducing the mycelial growth of P. capsici and P. parasitica. The treatments with T. aggressivum f. europaeum, T. longibrachiatum, and P. variotii substantially reduced the severity of the disease caused by P. capsici by 54, 76, and 70%, respectively, and of the disease caused by P. parasitica by 66, 55, and 64%, respectively. T. saturnisporum had the lowest values of disease reduction. Reinoculation with the four BCAs increased the control of both plant pathogens. Markedly different expression patterns were observed in the genes CaBGLU, CaRGA1, and CaSAR8.2. Based on the results, all four BCAs under study could be used as a biological alternative to chemicals for the control of P. capsici and P. parasitica in pepper with a high success rate.

show abstract

Reduction of Phytophthora Capsici Oospores Viability by Gases Released During Soil Biosolarization of Protected Pepper Crops in Southeastern Spain

Cited by 2 publications

References 0 publications

Gases Released During Soil Biodisinfestation of Pepper Greenhouses Reduce Survival of Phytophthora capsici Oospores in Northern Spain

Gases Released During Soil Biodisinfestation of Pepper Greenhouses Reduce Survival of Phytophthora capsici Oospores in Northern Spain

Biocontrol of Diseases Caused by Phytophthora capsici and P. parasitica in Pepper Plants

Contact Info

Product

Resources

About