Reduction of plasma granzyme A correlates with severity of sepsis in burn patients

Accardo-Palumbo, Antonina; D’Amelio, L.; Pileri, D.; D’Arpa, N.; Mogavero, R.; Amato, Giorgio; Cataldo, Vince D.; Napoli, B.; Ciccia, Francesco; Lombardo, Carla; Conte, Fábio F.

doi:10.1016/j.burns.2009.11.009

Cited by 16 publications

(11 citation statements)

References 32 publications

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“…In addition, granzyme H (GZMH), which plays a role in innate immune response, is down-regulated at the gene expression level. This observation is in accordance with previous research on granzyme A (Accardo-Palumbo et al, 2010).…”

Section: Idsupporting

confidence: 94%

Screening of differentially expressed genes between multiple trauma patients with and without sepsis

Pan

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The purpose of this study was to identify critical genes associated with septic multiple trauma by comparing peripheral whole blood samples from multiple trauma patients with and without sepsis. A microarray data set was downloaded from the Gene Expression Omnibus (GEO) database. This data set included 70 samples, 36 from multiple trauma patients with sepsis and 34 from multiple trauma patients without sepsis (as a control set). The data were preprocessed, and differentially expressed genes (DEGs) were then screened for using packages of the R language. Functional analysis of DEGs was performed with DAVID. Interaction networks were then established for the most up-and downregulated genes using HitPredict. Pathway-enrichment analysis was conducted for genes in the networks using WebGestalt. Fifty-eight DEGs were identified. The expression levels of PLAU (down-regulated) and MMP8 (up-regulated) presented the largest fold-changes, and interaction networks were established for these genes. Further analysis revealed that PLAT (plasminogen activator, tissue) and SERPINF2 (serpin peptidase inhibitor, clade F, member 2), which interact with PLAU, play important roles in the pathway of the component and coagulation cascade. We hypothesize that PLAU is a major regulator of the component and coagulation cascade, and down-regulation of PLAU results in dysfunction of the pathway, causing sepsis.

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Section: Idsupporting

confidence: 94%

Screening of differentially expressed genes between multiple trauma patients with and without sepsis

Pan

et al. 2014

Genet. Mol. Res.

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“…This may explain why CD8 + T cells and gdT cell counts and cytotoxic loading decreased significantly three days after trauma, following surgery, transfusion and anaesthesia. Meanwhile, gdT cells migrate to the injury sites after trauma, reducing the count in peripheral blood [7,10,19].…”

Section: Discussionmentioning

confidence: 99%

“…Functional impairment of NK cells, gdT cells and CTLs has been involved in susceptibility to bacterial infections of hosts that experienced trauma [10,32].…”

Section: Discussionmentioning

confidence: 99%

“…Cytotoxic CD8 + T lymphocytes (CTLs), NK and gdT cells exert their biological activity through two distinct mechanisms [5][6][7]: synthesis and release of soluble mediators such as cytokines, and release of cytotoxic granules inducing apoptosis in neighbouring cells [8,9]. The density of granules and their content in cytotoxic mediators such as perforin and granzymes in CTLs, NK cells, and gdT cells might indicate the potential activity of these cells [10], and overall status of host cellular immunity [11].…”

Section: Introductionmentioning

confidence: 99%

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Decreased levels of perforin-positive lymphocytes are associated with posttraumatic complications in patients with major trauma

Hua

Zhang

Chen

et al. 2014

Injury

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“…Patients with severe burns require immediate specialized care in order to minimize morbidity and mortality. It has been demonstrated that a large thermal injury induces a state of immunosuppression that predisposes the patient to infectious complications [1]. It causes immune dysfunction involving both pro- and anti-inflammatory responses.…”

Section: Introductionmentioning

confidence: 99%

Reduction of Plasma Gelsolin Levels Correlates with Development of Multiple Organ Dysfunction Syndrome and Fatal Outcome in Burn Patients

Huang

Yao

et al. 2011

PLoS ONE

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BackgroundDepletion of the circulating actin-binding protein, plasma gelsolin (pGSN) has been described in critically ill surgical patients. We hypothesized that the extent of pGSN reduction might correlate with different outcome of burn patients. The study was performed to evaluate the prognostic implications of pGSN levels on the development of multiple organ dysfunction syndrome (MODS) and fatal outcome in a group of severely burn patients.Methods and Findings95 patients were included, and they were divided into three groups with different burn area: group I (n = 33), group II (n = 32) and group III (n = 30). According to whether there was development of MODS or not, patients were divided into MODS group (n = 28) and none-MODS group (n = 67); then the patients with MODS were further divided into non-survivor group (n = 17) and survivor group (n = 11). The peripheral blood samples were collected on postburn days (PBD) 1, 3, 7, 14, and 21. The levels of pGSN were determined and T cells were procured from the blood. The contents of cytokines (IL-2, IL-4 and IFN-γ) released by T cells were also measured. The related factors of prognosis were analyzed by using multivariate logistic regression analysis. The results showed that pGSN concentrations, as well as the levels of IL-2 and IFN-γ, decreased markedly on PBD 1–21, whereas, the levels of IL-4 increased markedly in all burn groups as compared with normal controls (P<0.05 or P<0.01), and there were obviously differences between group I and group III (P<0.05 or P<0.01). The similar results were found in MODS patients and the non-survivor group as compared with those without MODS and the survival group on days 3–21 postburn (P<0.05 or P<0.01). Moreover, as the pGSN levels decreased, the incidence of septic complication as well as MODS remarkably increased.ConclusionspGSN levels appear to be an early prognostic marker in patients suffering from major burns.

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Reduction of plasma granzyme A correlates with severity of sepsis in burn patients

Cited by 16 publications

References 32 publications

Screening of differentially expressed genes between multiple trauma patients with and without sepsis

Screening of differentially expressed genes between multiple trauma patients with and without sepsis

Decreased levels of perforin-positive lymphocytes are associated with posttraumatic complications in patients with major trauma

Reduction of Plasma Gelsolin Levels Correlates with Development of Multiple Organ Dysfunction Syndrome and Fatal Outcome in Burn Patients

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