Reduction of Selenate and Selenite to Elemental Selenium by a
            <i>Pseudomonas stutzeri</i>
            Isolate

Lortie, Louis-André; Gould, W. Douglas; Rājan, S. S. S.; McCready, R. G. L.; Cheng, K.-J.

doi:10.1128/aem.58.12.4042-4044.1992

Cited by 194 publications

(99 citation statements)

References 13 publications

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“…Because S. maltophilia is incapable of using either SeO 4 2or SeO 3 2as a terminal electron acceptor during anaerobic growth (data not shown), the reduction of both Se oxyanions may be a mechanism of resistance. Lortie et al (1992) reported that the aerobic reduction of SeO 4 2and SeO 3 2to Se∞ by a Pseudomonas stutzeri isolate functioned as a detoxification mechanism as there was no evidence of dissimilatory Se reduction. In S. maltophilia a detoxification mechanism may be responsible, but it appears unlikely as the formation of Se∞ occurs during stationary phase and not during exponential growth.…”

Section: Reduction Of Selenate and Selenitementioning

confidence: 99%

Transformations of selenate and selenite by Stenotrophomonas maltophilia isolated from a seleniferous agricultural drainage pond sediment

Dungan¹,

Yates²,

Frankenberger³

2003

Environmental Microbiology

125

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A Gram-negative bacterium, identified as Stenotrophomonas maltophilia by fatty acid analysis and 16S rRNA sequencing, was isolated from a seleniferous agricultural evaporation pond sediment collected in the Tulare Lake Drainage District, California. In cultures exposed to the atmosphere, the organism reduces selenate (SeO4(2-)) and selenite (SeO3(2-)) to red amorphous elemental selenium (Se degrees ) only upon reaching stationary phase, when O2 levels are less than 0.1 mg l(-1). In 48 h, S. maltophilia removed 81.2% and 99.8% of added SeO4(2-) and SeO3(2-) (initial concentration of 0.5 mM), respectively, from solution. Anaerobic growth experiments revealed that the organism was incapable of using SeO4(2-), SeO3(2-), SO4(2-) or NO3- as a terminal electron acceptor. Transmission electron microscopy of cultures spiked with either Se oxyanion were found to contain spherical extracellular deposits. Analysis of the deposits by energy-dispersive X-ray spectroscopy revealed that they consist of Se. Furthermore, S. maltophilia was active in producing volatile alkylselenides when in the presence of SeO4(2-) and SeO3(2-). The volatile products were positively identified as dimethyl selenide (DMSe), dimethyl selenenyl sulphide (DMSeS) and dimethyl diselenide (DMDSe) by gas chromatography-mass spectrometry. Our findings suggest that this bacterium may contribute to the biogeochemical cycling of Se in seleniferous evaporation pond sediments and waters. This organism may also be potentially useful in a bioremediation scheme designed to treat seleniferous agricultural wastewater.

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Section: Reduction Of Selenate and Selenitementioning

confidence: 99%

Transformations of selenate and selenite by Stenotrophomonas maltophilia isolated from a seleniferous agricultural drainage pond sediment

Dungan¹,

Yates²,

Frankenberger³

2003

Environmental Microbiology

125

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“…Redox poise is maintained through the removal of excess electrons via a membrane-bound, FADH 2 -dependent metal reductase (MORase). Wolinella succinogenes [64], Desulfovibrio desulfuricans [65], speci¢c strains of Pseudomonas stutzeri [66] and E. cloacae [67] are capable of reducing sub-millimolar levels of Se(VI) to Se(0). This activity could be attributed to other reductases that have a broad range substrate speci¢city.…”

Section: Toxicitymentioning

confidence: 99%

Bacterial respiration of arsenic and selenium

Stolz

1999

FEMS Microbiology Reviews

148

207

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Oxyanions of arsenic and selenium can be used in microbial anaerobic respiration as terminal electron acceptors. The detection of arsenate and selenate respiring bacteria in numerous pristine and contaminated environments and their rapid appearance in enrichment culture suggest that they are widespread and metabolically active in nature. Although the bacterial species that have been isolated and characterized are still few in number, they are scattered throughout the bacterial domain and include Gram-positive bacteria, beta, gamma and epsilon Proteobacteria and the sole member of a deeply branching lineage of the bacteria, Chrysiogenes arsenatus. The oxidation of a number of organic substrates (i.e. acetate, lactate, pyruvate, glycerol, ethanol) or hydrogen can be coupled to the reduction of arsenate and selenate, but the actual donor used varies from species to species. Both periplasmic and membrane-associated arsenate and selenate reductases have been characterized. Although the number of subunits and molecular masses differs, they all contain molybdenum. The extent of the environmental impact on the transformation and mobilization of arsenic and selenium by microbial dissimilatory processes is only now being fully appreciated.

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“…Recently, some bacterial strains which can effectively reduce selenate into selenite or elemental selenium have been reported [2,[6][7][8]12]. They are all gram-negative bacteria.…”

Section: Flavobacterium Okeanokoites Saccharococcus Thermophilus Macrmentioning

confidence: 99%

Factors affecting soluble selenium removal by a selenate-reducing bacterium Bacillus sp. SF-1

Kashiwa

Nishimoto

Takahashi

et al. 2000

Journal of Bioscience and Bioengineering

122

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Reduction of Selenate and Selenite to Elemental Selenium by a Pseudomonas stutzeri Isolate

Cited by 194 publications

References 13 publications

Transformations of selenate and selenite by Stenotrophomonas maltophilia isolated from a seleniferous agricultural drainage pond sediment

Transformations of selenate and selenite by Stenotrophomonas maltophilia isolated from a seleniferous agricultural drainage pond sediment

Bacterial respiration of arsenic and selenium

Factors affecting soluble selenium removal by a selenate-reducing bacterium Bacillus sp. SF-1

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