Reduction of spiked porcine circovirus during the manufacture of a Vero cell-derived vaccine

Lackner, Cornelia; Leydold, Sandra M.; Modrof, Jens; Farcet, Maria R.; Grillberger, Leopold; Schäfer, Björn Malte; Anderle, Heinz; Kreil, Thomas R.

doi:10.1016/j.vaccine.2014.02.011

Cited by 11 publications

(4 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the next day, cells were treated with peptides at the indicated time points and infected with adenovirus (MOI of 0.5) in a volume of 800 μl medium. After 6 days, viral DNA was extracted from 70 μl of the supernatants using QIAamp Viral RNA Mini kit (Qiagen), which also allows purification of viral DNA (Lackner et al, 2014). Viral DNA was eluted with 60 μl elution buffer.…”

Section: Adenovirusmentioning

confidence: 99%

A Novel, Broad-Acting Peptide Inhibitor of Double-Stranded DNA Virus Gene Expression and Replication

et al. 2020

View full text Add to dashboard Cite

Viral infections are a global disease burden with only a limited number of antiviral agents available. Due to newly emerging viral pathogens and increasing occurrence of drug resistance, there is a continuous need for additional therapeutic options, preferably with extended target range. In the present study, we describe a novel antiviral peptide with broad activity against several double-stranded DNA viruses. The 22-mer peptide TAT-I24 potently neutralized viruses such as herpes simplex viruses, adenovirus type 5, cytomegalovirus, vaccinia virus, and simian virus 40 in cell culture models, while being less active against RNA viruses. The peptide TAT-I24 therefore represents a novel and promising drug candidate for use against double-stranded DNA viruses.

show abstract

Section: Adenovirusmentioning

confidence: 99%

A Novel, Broad-Acting Peptide Inhibitor of Double-Stranded DNA Virus Gene Expression and Replication

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Since that incident, the Food and Drug Administration (FDA) and several research institutions began investigating the source of the vaccine contamination and assessing the safety of the vaccine. Their results indicated that several cell lines, virus seeds and vaccines were contaminated by PCV1 and/or PCV2 (70)(71)(72)(73)(74)(75). Porcinederived commercial pepsin product used for cell culture and vaccine production was considered as the main source of vaccine contamination (76).…”

Section: Pcvs In Biological Productsmentioning

confidence: 99%

Reservoirs of Porcine Circoviruses: A Mini Review

Zhai

Wei

et al. 2019

Front. Vet. Sci.

View full text Add to dashboard Cite

Porcine circovirus (PCV) is one of the smallest known DNA viruses in mammals. At present, PCVs are divided into three species, PCV1, PCV2, and PCV3. PCV1 and PCV2 were found in the 1970s and the 1990s, respectively, whereas PCV3 was discovered recently in 2016. PCV1 does not cause diseases in pigs. However, PCV3, similar to PCV2, is reported to be associated with several swine diseases, including porcine dermatitis and nephropathy syndrome (PDNS) and reproductive failure. PCVs are very common in domestic pigs as well as wild boars. However, PCVs have been occasionally isolated from non-porcine animals, including ruminants (such as cattle, goats, wild chamois, and roe deers), rodents (such as NMRI mice, BALB/c mice, Black C57 mice, ICR mice, Mus musculus, and Rattus rattus), canines (such as dogs, minks, foxes, and raccoon dogs), insects (such as flies, mosquitoes, and ticks), and shellfish. Moreover, PCVs are frequently reported in biological products, including human vaccines, animal vaccines, porcine-derived commercial pepsin products, and many cell lines. PCVs are also abundant in the environment, including water samples and air samples. Interestingly, PCV1 and/or PCV2 antibody or antigen has also been detected in sera, stool samples and respiratory swab samples of human, revealing zoonotic potential of PCVs. Thus, PCVs inhabit many types of reservoirs. In this review, we summarize the reservoirs of PCVs, and this information would be helpful in understanding the natural circulating status and possible cross-species transmission of PCVs.

show abstract

“…Recent guidance from the European Medicines Agency [35] recommends the use of two complementary virus reduction steps for trypsin, including a final inactivation step of gamma, e-beam, or UV irradiation. UV sensitivity of PCV has been measured by Lackner et al [58] and Remington [85], with a D 10 range of 14-25 mJ/cm 2 . Although this range is large as a percentage, the moderate sensitivity reported by all investigators indicates that PCV can be readily inactivated by UV.…”

Section: Porcine Circovirusmentioning

confidence: 99%

Evaluating ultraviolet sensitivity of adventitious agents in biopharmaceutical manufacturing

Meunier

Sasges

Aucoin

2017

Journal of Industrial Microbiology and Biotechnology

View full text Add to dashboard Cite

Incidents of contamination in biopharmaceutical production have highlighted the need to apply alternative or supplementary disinfection techniques. Ultraviolet (UV) irradiation is a well-established method for inactivating a broad range of microorganisms, and is therefore a good candidate as an orthogonal technique for disinfection. To apply UV as a safeguard against adventitious agents, the UV sensitivity of these target agents must be known so that the appropriate dose of UV may be applied to achieve the desired level of inactivation. This document compiles and reviews experimentally derived 254 nm sensitivities of organisms relevant to biopharmaceutical production. In general, different researchers have found similar sensitivity values despite a lack of uniformity in experimental design or standardized quantification techniques. Still, the lack of consistent methodologies has led to suspicious UV susceptibilities in certain instances, justifying the need to create a robust collection of sensitivity values that can be used in the design and sizing of UV systems for the inactivation of adventitious agents.

show abstract

Reduction of spiked porcine circovirus during the manufacture of a Vero cell-derived vaccine

Cited by 11 publications

References 26 publications

A Novel, Broad-Acting Peptide Inhibitor of Double-Stranded DNA Virus Gene Expression and Replication

A Novel, Broad-Acting Peptide Inhibitor of Double-Stranded DNA Virus Gene Expression and Replication

Reservoirs of Porcine Circoviruses: A Mini Review

Evaluating ultraviolet sensitivity of adventitious agents in biopharmaceutical manufacturing

Contact Info

Product

Resources

About