Reduction of the spherical aberration effect in high-numerical-aperture optical scanning instruments

Escobar, Isabel; Saavedra, Genaro; Martı́nez-Corral, Manuel; Láncis, Jesús

doi:10.1364/josaa.23.003150

Cited by 28 publications

(14 citation statements)

References 37 publications

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“…With larger axial ranges, however, it is generally anticipated that spherical aberration will become more important. In such cases, it might prove useful to utilize a reverse Gaussian aperture 44 to preserve the resolution over the entire axial range at the expense of a mildly reduced resolution at the inherent focal plane.…”

Section: Discussionmentioning

confidence: 99%

Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity

et al. 2008

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The dynamic ability of neuronal dendrites to shape and integrate synaptic responses is the hallmark of information processing in the brain. Effectively studying this phenomenon requires concurrent measurements at multiple sites on live neurons. Significant progress has been made by optical imaging systems which combine confocal and multiphoton microscopy with inertia-free laser scanning. However, all systems developed to date restrict fast imaging to two dimensions. This severely limits the extent to which neurons can be studied, since they represent complex threedimensional (3D) structures. Here we present a novel imaging system that utilizes a unique arrangement of acousto-optic deflectors to steer a focused ultra-fast laser beam to arbitrary locations in 3D space without moving the objective lens. As we demonstrate, this highly versatile randomaccess multiphoton microscope supports functional imaging of complex 3D cellular structures such as neuronal dendrites or neural populations at acquisition rates on the order of tens of kilohertz.

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Section: Discussionmentioning

confidence: 99%

Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity

et al. 2008

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“…After straightforward maths, the complex amplitude distribution along the optical axis is given by [27] U w 40 , w 20…”

Section: The Mismatch Index-induced Spherical Aberrationmentioning

confidence: 99%

Shaded-Mask Filtering for Extended Depth-of-Field Microscopy

Escobar¹,

Saavedra²,

Martı́nez-Corral³

et al. 2013

Journal of information and communication convergence engineerin

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This paper proposes a new spatial filtering approach for increasing the depth-of-field (DOF) of imaging systems, which is very useful for obtaining sharp images for a wide range of axial positions of the object. Many different techniques have been reported to increase the depth of field. However the main advantage in our method is its simplicity, since we propose the use of purely absorbing beam-shaping elements, which allows a high focal depth with a minimum modification of the optical architecture. In the filter design, we have used the analogy between the axial behavior of a system with spherical aberration and the transverse impulse response of a 1D defocused system. This allowed us the design of a ring-shaded filter. Finally, experimental verification of the theoretical statements is also provided.

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“…The spherical aberration can also be explained by saying that the deviation of the principal surface from its ideal shape causes the focal length to slightly vary with the radius of zone of the aperture. There has been a significant number of studies involving diffractive monochromatic aberrations with different aperture systems and techniques [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Spherical Aberration of Point Spread Function with Asymmetric Pupil Mask

Reddy

Dasari

2016

Advances in Optical Technologies

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Point spread function underneath spherical wave aberration with antiphase apodization has been obtained by one-dimensional pupil mask functions. In the presence of spherical aberration, suppression of optical side-lobes has increased on one side of the point spread function with the width of the periphery strips within the pupil mask. On introducing wave aberration effect, there exists dependence of the lateral resolution of central peak of the asymmetric point spread function on the amount of amplitude masking. However, the magnitude of intensity of central peak is originated be to amplified by the highest degree of amplitude and phase masking. Additionally, for aberrated asymmetric PSF, FWHM increases and it further decreases with the control parameters of amplitude and phase mask. The magnitude of this corollary can quantify the super resolution of diffracted structures under spherical aberration.

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Reduction of the spherical aberration effect in high-numerical-aperture optical scanning instruments

Cited by 28 publications

References 37 publications

Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity

Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity

Shaded-Mask Filtering for Extended Depth-of-Field Microscopy

Spherical Aberration of Point Spread Function with Asymmetric Pupil Mask

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