Reduction of Toxicity of Cationic Macromolecules by Complexing with Anionic Derivatives of Synthetic Polyglucoses

Mora, Peter T.; Young, Bobby G.; Shear, M. J.

doi:10.1038/184431a0

Cited by 23 publications

(7 citation statements)

References 5 publications

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“…Since the lytic protein is cationic under the experimental conditions used it is conceivable that the complexes formed are with anionic derivatives of the cell wall and the dissociation of the bonds by NaCl is similar to that reported by Mora, Young & Shear (1959) on the complexing of cationic macromolecules.…”

Section: S Uureus (Sv)supporting

confidence: 56%

The Role of NaCl in the Lysis of Staphylococcus aureus by Lysostaphin

Schindler

1965

Journal of General Microbiology

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SUMMARYLysostaphin attacked both viable staphylococci and the mucopeptide portion of the staphylococcal cell wall. In the absence of salts, lysostaphin activity could only be recovered from the particulate portion of the lysed cell after centrifugation, whereas in the absence of salts its action on the mucopeptide resulted in a recovery of active material in both the sediment and the supernatant fluid. It appears from these observations that lysostaphin is complexed with its substrate and that NaCl is required to break the complex.

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Section: S Uureus (Sv)supporting

confidence: 56%

The Role of NaCl in the Lysis of Staphylococcus aureus by Lysostaphin

Schindler

1965

Journal of General Microbiology

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“…In the second, the stronger anionic polyglucose sulphate formed complexes with the cationic macromolecule, and thus liberated the tumourdamaging site of the bacterial polysaccharide. This mechanism is similar to the one we proposed on the reversible inhibition of enzymes , and on the blocking of toxicity (Mora, Young & Shear, 1959).…”

Section: Discussionmentioning

confidence: 58%

“…We reported previously on the blocking of enzyme activity and of toxicity through interaction of oppositely charged macromolecules (Mora & Young, 1958 ;Mora, Young & Shear, 1959). This approach has been extended to the study of the interaction of a bacterial lipopolysaccharide preparation from Serratia marcescens of negative net charge near neutral pH, with positive (cationic) macromolecules.…”

Section: Introductionmentioning

confidence: 99%

Interaction of an Endotoxin with Cationic Macromolecules

Mora

Young

1961

Journal of General Microbiology

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SUMMARYThe interaction of the anionic lipopolysaccharide complex isolated from a Gramnegative bacterium (Serratia marcescens) was studied in aqueous and saline solution with several cationic macromolecules, with the object of selectively inhibiting certain of the biological activities of this polysaccharide endotoxin. Incubation with lysozyme decreased the pyrogenic activity, while the tumour-damaging ability of this polysaccharide remained high. This was in contrast to interaction with RNase and with polymyxin B, in which cases the tumour-damaging activity was decreased while the pyrogenic activity was not affected. Ultracentrifuge experiments indicated that both the tumour-necrotic and especially the fever-producing fractions of this bacterial polysaccharide preparation are of much higher molecular weight ( > 108) than the major component (3-4S), which is either inactive or of low activity.The highest molecular weight fever-producing components appeared to be broken down in an enzymic type of process by the lysozyme.

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“…90 Sulfate and carboxyl derivatives of polyglucose have a similar protective effect against polymyxin and, to a lesser degree, neomycin and streptomycin. 152 Heparin apparently counteracts the acute toxic effects of neomycin without producing an equivalent loss in antibiotic activity. 89 In recent years, further data on "complexing" by heparin in vitro have been reported.…”

Section: Formation Of "Complexes"mentioning

confidence: 99%