Reduction of transepidermal water loss by oral intake of glucosylceramides in patients with atopic eczema

Miyanishi, Keita; Shiono, Nobuko; Shirai, Hisaya; Dombo, M.; Kimata, Hajime

doi:10.1111/j.1398-9995.2005.00915.x

Cited by 32 publications

(24 citation statements)

References 6 publications

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“…Dietary plant sphingolipids with sphingoid bases distinct from those found in mammals are able to prevent the formation of aberrant crypt foci in 1,2-dimethylhydrazine-treated mice (7,8). In addition, the beneficial effects of dietary plant sphingolipids have been reported in patients with atopic eczema and in model mice of skin damage, although the compositions of sphingoid bases in the stratum corneum of mammals are quite different from those of dietary glucosylceramide derived from plant sources (9)(10)(11). Our previous findings suggest that the skin barrier-improving effect of dietary sphingolipids might be due to the activation of ceramide synthesis in the skin, rather than to the direct reutilization of dietary sphingolipids (12).…”

mentioning

confidence: 97%

Selective Absorption of Dietary Sphingoid Bases from the Intestine via Efflux by P-Glycoprotein in Rats

Fujii

Manabe

Aida

et al. 2017

J Nutr Sci Vitaminol

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Summary Various physiological functions of dietary sphingolipids, such as preventing inflammation and improving the skin barrier function, have been recently demonstrated. The sphingolipid most commonly used as a foodstuff is glucosylceramide from plant sources, which is composed of sphingoid bases that are distinctive from those found in mammals. Although the structure of sphingoid bases in higher plants is more complicated than the structure of those in mammals, the fate of dietary sphingolipids of plant origin is still not understood. In the present study, we investigated the absorption of 4,8-sphingadienine that originated from maize glucosylceramide in the rat intestine by using a lipid absorption assay of lymph collected from the thoracic duct. The cumulative recovery of 4,8-sphingadienine in the lymph was lower than that of sphingosine. Verapamil, a P-glycoprotein inhibitor, significantly increased the absorption of 4,8-sphingadienine but did not affect the absorption of sphingosine. Plant-derived sphingoid bases were detected in the ceramide fraction of lymph fluid by using liquid chromatography-mass spectrometry analysis. These results indicate that 4,8-sphingadienine that originates from the glucosylceramide of higher plants is poorly absorbed in the intestine because of efflux by P-glycoprotein and can be incorporated into a ceramide moiety, at least in part, in intestinal endothelial cells. Key Words sphingolipids, sphingoid bases, sphingadienine, intestinal absorption, P-glycoprotein Sphingolipids are ubiquitous in all eukaryotic organisms and constitute a family of compounds that have a sphingoid base with an amide-linked fatty acid and a polar head group. Sphingolipids, having various structures of sphingoid bases, are ingested daily from foodstuffs because of the diverse structures of sphingoid bases that occur in nature. The most common sphingoid base of mammalian sphingolipids is sphingosine (trans-4-sphingenine, d18:1 4t ). Smaller amounts of other sphingoid bases such as sphinganine (dihydrosphingosine, d18:0) and phytosphingosine (4-hydroxysphinganine, t18:0) are encountered frequently. In higher plants, the structures of sphingoid bases are more complicated than in mammals because they can be desaturated at the C8-position by D8-sphingolipid desaturase, yielding cis-and trans-isomers of D8-unsaturated sphingoid bases (d18:2 4t,8c (t) ). Dietary sphingolipids from various sources have been reported to have beneficial effects such as preventing cancer (1-3), reducing inflammatory responses (4), and lowering levels of plasma lipids (5, 6). Dietary plant sphingolipids with sphingoid bases distinct from those found in mammals are able to prevent the formation of aberrant crypt foci in 1,2-dimethylhydrazine-treated mice (7,8). In addition, the beneficial effects of dietary plant sphingolipids have been reported in patients with atopic eczema and in model mice of skin damage, although the compositions of sphingoid bases in the stratum corneum of mammals are quite different from those of dietary...

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mentioning

confidence: 97%

Selective Absorption of Dietary Sphingoid Bases from the Intestine via Efflux by P-Glycoprotein in Rats

Fujii

Manabe

Aida

et al. 2017

J Nutr Sci Vitaminol

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“…13) It has also been reported that oral intake of glucosylceramide reduced transepidermal water loss (TEWL) 14) in normal adult 12) or in AD patients. 15,16) A. konjac, a rich source of glucocylceramide, has been eaten as food for a long time in Japan. Additionally, A. konjac is extremely safe with no allergenic effects.…”

Section: Introductionmentioning

confidence: 99%

Oral Intake of Glucosylceramide Improves Relatively Higher Level of Transepidermal Water Loss in Mice and Healthy Human Subjects

Uchiyama

Nakano

Ueda

et al. 2008

JOURNAL OF HEALTH SCIENCE

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We examined the effect of oral intake of pure glucosylceramide derived from konjac extract on skin barrier function evaluated by transepidermal water loss (TEWL) in hairless mice with sodium dodecyl sulfate (SDS)-induced skin roughness. The difference of TEWL between SDS-treated site and untreated sites in the pure glucosylceramide-fed group was significantly lower than that in control group on day 14 of ingestion. We investigated interleukin-1α (IL-1α) production in the hairless mouse skin, and it was significantly lower in the glucosylceramide-fed group than that of control animals. This reduced IL-1α production should contribute to improvement of skin barrier function. To investigate the effect of oral intake of glucosylceramide in human, we conducted a randomized double-blind placebo-controlled study including 100 healthy subjects whose TEWL in cheek was relatively high. As a result, cheek TEWL was significantly lower in the test product group as compared with the control group in weeks 8 and 12 of ingestion (p = 0.023 and p = 0.002 respectively).

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“…Alternatively, another 18 elderly healthy people or 18 elderly AD patients first viewed a control film featuring weather information at 8:00 am, and after 2 weeks they viewed a humorous film (The Best Bits of Mr. Bean) at 8:00 am. Before and immediately after each viewing, saliva was collected and TEWL values on the back of the neck were measured by Tewameter (C + K Co. Ltd., Kolen, Germany), as previously reported (18). TEWL values were registered in g/m 2 /h after equilibration of the probe.…”

Section: Methodsmentioning

confidence: 99%

Elevation of Testosterone and Reduction of Transepidermal Water Loss by Viewing a Humorous Film in Elderly Patients with Atopic Dermatitis

Kimata¹

2007

Acta Med. (Hradec Kralove, Czech Repub.)

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Skin barrier function was impaired and transepidermal water loss (TEWL) values were increased in patients with atopic dermatitis (AD) (2). TEWL values were also increased in elderly healthy people, thus elderly patients with AD had higher TEWL values (7). AD patients were vulnerable to stress (4), and stress impaired barrier function and increased TEWL values (5). Stress also augmented allergic responses, while relaxation by viewing a humorous film reduced allergic responses in AD patients (13,14). In addition, inhalation of odorant with a sedative effect prevented the stress-induced delay of skin barrier recovery (8).It was reported that serum levels of testosterone were decreased in male patients with AD, and testosterone was involved in improvement of TEWL (1, 9). Stress decreased serum testosterone levels (3). Since serum testosterone levels were correlated with salivary testosterone levels, we studied whether viewing a humorous film could elevate salivary testosterone levels and reduce TEWL values in elderly AD patients (21). Materials and methodsAfter obtaining informed consents from subjects, 36 elderly healthy people (36 male, mean age 70 years, range 65-76 years) and 36 elderly patients with mild AD (scoring atopic dermatitis [SCORAD] index: mean 9, 95 % CI 7-11) (36 male, mean age 70 years, range 65-76 years) were studied (16). All of the AD patients were treated with an application of pinetar (Yoshida Pharmaceutical Co. Ltd. Tokyo, Japan) mixed with zinc oxide ointment, but without oral medication (4). The application of ointment was stopped 2 days before the study. Neither healthy people nor AD patients had any endocrinological diseases or skin infection.Study was conducted by cross-over style. 18 randomly assigned elderly healthy people or 18 elderly AD patients viewed a humorous film (The Best Bits of Mr. Bean, 72 min, Universal studios, 1996) at 8:00 am. After 2 weeks, they viewed a control non-humorous film featuring weather information (72 min) at 8:00 am. Alternatively, another 18 elderly healthy people or 18 elderly AD patients first viewed a control film featuring weather information at 8:00 am, and after 2 weeks they viewed a humorous film (The Best Bits of Mr. Bean) at 8:00 am. Before and immediately after each viewing, saliva was collected and TEWL values on the back of the neck were measured by Tewameter (C + K Co. Ltd., Kolen, Germany), as previously reported (18). TEWL values were registered in g/m 2 /h after equilibration of the probe. Testosterone levels in fasting saliva were measured by EIA (Testosterone Kit, Oxford Biomedical Research, 135 ORIGINAL ARTICLE ELEVATION OF TESTOSTERONE AND REDUCTION OF TRANSEPIDERMAL WATER LOSS BY VIEWING A HUMOROUS FILM IN ELDERLY PATIENTS WITH ATOPIC DERMATITIS Hajime KimataMoriguchi-Keijinkai Hospital, Osaka Prefecture, Japan: Department of Allergy Summary:The effect of viewing a humorous film on salivary testosterone levels and transepidermal water loss (TEWL) values on the back of the neck in 36 elderly healthy people (36 male, mean 70 years...

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Reduction of transepidermal water loss by oral intake of glucosylceramides in patients with atopic eczema

Cited by 32 publications

References 6 publications

Selective Absorption of Dietary Sphingoid Bases from the Intestine via Efflux by P-Glycoprotein in Rats

Selective Absorption of Dietary Sphingoid Bases from the Intestine via Efflux by P-Glycoprotein in Rats

Oral Intake of Glucosylceramide Improves Relatively Higher Level of Transepidermal Water Loss in Mice and Healthy Human Subjects

Elevation of Testosterone and Reduction of Transepidermal Water Loss by Viewing a Humorous Film in Elderly Patients with Atopic Dermatitis

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