Reduction of Zika virus infectivity in platelet concentrates after treatment with ultraviolet C light and in plasma after treatment with methylene blue and visible light

Fryk, Jesse J.; Marks, Denese C.; Hobson‐Peters, Jody; Watterson, Daniel; Hall, Roy A.; Young, Paul R.; Reichenberg, Stefan; Tolksdorf, Frank; Sumian, Chryslain; Gravemann, Ute; Seltsam, Axel; Faddy, Helen M.

doi:10.1111/trf.14256

Cited by 34 publications

(44 citation statements)

References 36 publications

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“…Hemolytic and lipemic units were discarded. In accordance with the instructions for use of the THERAFLEX UV‐Platelets system, the target platelet concentration was 0.8 to 1.4 × 10/mL and the target plasma content was 30% to 40%. Air was removed from the containers and the PCs were UVC treated as described in detail below.…”

Section: Methodsmentioning

confidence: 99%

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Bacterial inactivation of platelet concentrates with the THERAFLEX UV‐Platelets pathogen inactivation system

et al. 2018

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BACKGROUND The THERAFLEX UV‐Platelets system (Maco Pharma) uses ultraviolet C (UVC) light for pathogen inactivation (PI) of platelet concentrates (PCs) without any additional photoactive compound. The aim of the study was to systematically investigate bacterial inactivation with this system under conditions of intended use. STUDY DESIGN AND METHODS The robustness of the system was evaluated by assessing its capacity to inactivate high concentrations of different bacterial species in accordance with World Health Organization guidelines. The optimal use of the PI system was explored in time‐to‐treatment experiments by testing its ability to sterilize PCs contaminated with low levels of bacteria on the day of manufacture (target concentration, 100 colony‐forming units/unit). The bacteria panel used for spiking experiments in this study included the World Health Organization International Repository Platelet Transfusion Relevant Reference Strains (n = 14), commercially available strains (n = 13), and in‐house clinical isolates (n = 2). RESULTS Mean log reduction factors after UVC treatment ranged from 3.1 to 7.5 and varied between different strains of the same species. All PCs (n = 12/species) spiked with up to 200 colony‐forming units/bag remained sterile until the end of storage when UVC treated 6 hours after spiking. UVC treatment 8 hours after spiking resulted in single breakthrough contaminations with the fast‐growing species Escherichia coli and Streptococcus pyogenes. CONCLUSION The UVC‐based THERAFLEX UV‐Platelets system efficiently inactivates transfusion‐relevant bacterial species in PCs. The comprehensive data from this study may provide a valuable basis for the optimal use of this UVC‐based PI system.

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Section: Methodsmentioning

confidence: 99%

“…It has been demonstrated that UVC treatment significantly reduces the infectivity of platelet products contaminated by disease‐causing bacteria, viruses, and protozoa. In addition, THERAFLEX UV‐Platelets inactivates contaminating donor WBCs while preserving platelet function and coagulation factors …”

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Bacterial inactivation of platelet concentrates with the THERAFLEX UV‐Platelets pathogen inactivation system

et al. 2018

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“… §The UVC transparency of the irradiation bags was increased during the development of the THERAFLEX UV‐Platelets system. The inactivation efficacy of a UVC dose of 0.2 J/cm 2 used as full dose in this study and other recent studies , is equivalent to a UVC dose of 0.3 J/cm 2 used in the early phase of development , …”

Section: Discussionmentioning

confidence: 51%

“…10 This system has been tested in various studies against a wide range of disease-causing viruses, bacteria, and protozoa. 8,[11][12][13][14][15] In particular, the UVC-based PI system was effective against porcine parvovirus (PPV) and encephalomyocarditis virus (EMCV), 7 two nonenveloped viruses listed among those model viruses recommended for the evaluation of pathogen inactivation and removal technologies. 16,17 Its efficacy against PPV and EMCV suggests that THERAFLEX UV-Platelets may also be able to inactivate other nonenveloped viruses.…”

Section: Conclusion: Theraflex Uv-platelets Effectivelymentioning

confidence: 99%

Ultraviolet C light efficiently inactivates nonenveloped hepatitis A virus and feline calicivirus in platelet concentrates

et al. 2018

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BACKGROUND Nonenveloped transfusion‐transmissible viruses such as hepatitis A virus (HAV) and hepatitis E virus (HEV) are resistant to many of the common virus inactivation procedures for blood products. This study investigated the pathogen inactivation (PI) efficacy of the THERAFLEX UV‐Platelets system against two nonenveloped viruses: HAV and feline calicivirus (FCV), in platelet concentrates (PCs). STUDY DESIGN AND METHODS PCs in additive solution were spiked with high titers of cell culture–derived HAV and FCV, and treated with ultraviolet C at various doses. Pre‐ and posttreatment samples were taken and the level of viral infectivity determined at each dose. For some samples, large‐volume plating was performed to improve the detection limit of the virus assay. RESULTS THERAFLEX UV‐Platelets reduced HAV titers in PCs to the limit of detection, resulting in a virus reduction factor of greater than 4.2 log steps, and reduced FCV infectivity in PCs by 3.0 ± 0.2 log steps. CONCLUSIONS THERAFLEX UV‐Platelets effectively inactivates HAV and FCV in platelet units.

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“…Andere umhüllte Viren wie Dengue Virus (DENV) 1-4, ChikungunyaVirus (CHIKV), Ross River Virus (RRV) und Zikavirus (ZIKV) wurden ebenfalls gut inaktiviert [128,131].…”

Section: Unerwünschte Effekte Der Pathogeninaktivierung Mit Mirasol®unclassified

Pathogen-Inaktivierungssysteme für Thrombozytenkonzentrate

2018

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Reduction of Zika virus infectivity in platelet concentrates after treatment with ultraviolet C light and in plasma after treatment with methylene blue and visible light

Cited by 34 publications

References 36 publications

Bacterial inactivation of platelet concentrates with the THERAFLEX UV‐Platelets pathogen inactivation system

Bacterial inactivation of platelet concentrates with the THERAFLEX UV‐Platelets pathogen inactivation system

Ultraviolet C light efficiently inactivates nonenveloped hepatitis A virus and feline calicivirus in platelet concentrates

Pathogen-Inaktivierungssysteme für Thrombozytenkonzentrate

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