Kunitz trypsin inhibitor (KTI), one of the main antinutritional factors of soybean (Maetens et al., 2018), has a severely hazardous influence on the digestive system and pancreas tissues (Liener, 1995). Its content in soybeans is 1.4% (Bode & Huber, 1992). Therefore, establishing comprehensive, specific, and efficient detection methods of KTI in soybeans appears to be particularly necessary (Alves et al., 2010).Enzyme-linked immunosorbent assay (ELISA), as a common method to detect KTI, has significant specificity and efficiency (Chen, Huang,