Reductive amination-assisted quantitation of tamoxifen and its metabolites by liquid phase chromatography tandem mass spectrometry

Liang, Shih‐Shin; Wang, Tsu‐Nai; Chiu, Chien‐Chih; Kuo, Po‐Lin; Huang, Mei-Fang; Liu, Meng-Chieh; Tsai, Eing‐Mei

doi:10.1016/j.chroma.2016.01.015

Cited by 7 publications

(2 citation statements)

References 26 publications

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“…The derivatized method modifies PKU biomarkers, including PHE and TYR using isotopic formaldehyde to generate h 2 -formaldehyde-modified PHE and TYR (as standards) and d 2 -formaldehyde-modified PHE and TYR (as internal standards). The internal standards were used to normalize PHE and TYR signals, [18][19][20][21][22][23] and formaldehydemodified PHE and TYR were detected by mass spectrometry signal enhancement. 24 We compared the concentrations of plasma on filter paper and crude plasma samples and used HPLC-MS/MS to monitor derivatized PHE and TYR in the plasma samples.…”

Section: Introductionmentioning

confidence: 99%

Quantification of derivatized phenylalanine and tyrosine in dried blood spots using liquid chromatography with tandem spectrometry for newborn screening of phenylketonuria

Duh,

Liang,

Shen

et al. 2024

Eur J Mass Spectrom (Chichester)

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Phenylketonuria (PKU) is an autosomal genetic disorder caused by a deficiency of the phenylalanine hydroxylase (PAH) enzyme. The lack of PAH results in the inability of phenylalanine (PHE) to transform into tyrosine (TYR). Consequently, this leads to the accumulation of PHE in the blood samples of newborns causing metabolic diseases such as irreversible neurological problems. An analysis was required for determining the values of PHE and TYR in blood samples from newborn babies. In this study, therefore, we developed a derivatized method to monitor PHE and TYR in plasma samples using liquid phase chromatography linked with quadrupole mass spectrometry. Accessible formaldehyde isotopes and cyanoborohydride were used to react with PHE and TYR amino groups to generate h2-formaldehyde-modified PHE and TYR (as standards) and d2-formaldehyde-modified PHE and TYR (as internal standards). We used tandem mass spectrometry for multiple reaction monitoring. We demonstrated a derivatized method suitable for the PKU screening of newborns. The recoveries for PHE and TYR were 85% and 90%, respectively. Furthermore, we compared the values of PHE and TYR in different human plasma sample storage methods, including direct plasma and dried blood spots, and the results showed no significant difference.

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Section: Introductionmentioning

confidence: 99%

Quantification of derivatized phenylalanine and tyrosine in dried blood spots using liquid chromatography with tandem spectrometry for newborn screening of phenylketonuria

Duh,

Liang,

Shen

et al. 2024

Eur J Mass Spectrom (Chichester)

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“…Previously, we developed a derivatized method to generate ISs beneficial for HPLC–MS/MS analyses [ 33 , 34 , 35 , 36 , 37 , 38 , 39 ]. Herein, we used isotopic formaldehyde and sodium cyanoborohydride to generate two methyl groups on the amine functional group of TRP, 5-HTP, and serotonin.…”

Section: Introductionmentioning

confidence: 99%

Assisted Reductive Amination for Quantitation of Tryptophan, 5-Hydroxytryptophan, and Serotonin by Ultraperformance Liquid Chromatography Coupled with Tandem Mass Spectrometry

et al. 2023

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Herein, we used isotopic formaldehyde and sodium cyanoborohydride via reductive amination to label two methyl groups on primary amine to arrange the standards (h2-formaldehyde-modified) and internal standards (ISs, d2-formaldehyde-modified) of tryptophan and its metabolites, such as serotonin (5-hydroxytryptamine) and 5-hydroxytryptophan. These derivatized reactions with a high yield are very satisfactory for manufacturing standards and ISs. This strategy will generate one or two methyl groups on amine to create different mass unit shifts with 14 vs. 16 or 28 vs. 32 in individual compounds for biomolecules with amine groups. In other words, multiples of two mass units shift are created using this derivatized method with isotopic formaldehyde. Serotonin, 5-hydroxytryptophan, and tryptophan were used as examples to demonstrate isotopic formaldehyde-generating standards and ISs. h2-formaldehyde-modified serotonin, 5-hydroxytryptophan, and tryptophan are standards to construct calibration curves, and d2-formaldehyde-modified analogs such as ISs spike into samples to normalize the signal of each detection. We utilized multiple reaction monitoring modes and triple quadrupole mass spectrometry to demonstrate the derivatized method suitable for these three nervous biomolecules. The derivatized method demonstrated a linearity range of the coefficient of determinations between 0.9938 to 0.9969. The limits of detection and quantification ranged from 1.39 to 15.36 ng/mL.

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Reductive amination derivatization for the quantification of garlic components by isotope dilution analysis

Lin

Huang

et al. 2017

Food Chemistry

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Reductive amination-assisted quantitation of tamoxifen and its metabolites by liquid phase chromatography tandem mass spectrometry

Cited by 7 publications

References 26 publications

Quantification of derivatized phenylalanine and tyrosine in dried blood spots using liquid chromatography with tandem spectrometry for newborn screening of phenylketonuria

Quantification of derivatized phenylalanine and tyrosine in dried blood spots using liquid chromatography with tandem spectrometry for newborn screening of phenylketonuria

Assisted Reductive Amination for Quantitation of Tryptophan, 5-Hydroxytryptophan, and Serotonin by Ultraperformance Liquid Chromatography Coupled with Tandem Mass Spectrometry

Reductive amination derivatization for the quantification of garlic components by isotope dilution analysis

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