Reductive and Non-reductive Mechanisms of Iron Assimilation by the Yeast Saccharomyces cerevisiae

Lesuisse, Emmanuel; Labbé, Pierre

doi:10.1099/00221287-135-2-257

Cited by 92 publications

(116 citation statements)

References 14 publications

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“…The existence of two mechanisms for iron uptake makes the specific study of non-reductive uptake somewhat complicated. Uptake kinetics do not fit well with simple (Lesuisse & Labbe, 1989). Reduction of siderophores at the cell surface is a low-affinity and low-specificity mechanism.…”

Section: Introductionmentioning

confidence: 97%

“…This siderophore can enter cells via an FOB receptor present at the cell surface. We showed that the siderophore transport system of S. cerevisiae is specific, saturable, energydependent and has a high affinity for FOB (Lesuisse & Labbe, 1989). We called this an ' opportunistic strategy of iron uptake ' because S. cerevisiae can use siderophores excreted by other micro-organisms although it does not produce any itself.…”

Section: Introductionmentioning

confidence: 99%

“…The non-reductive mechanism requires that a ferric complex enters the cells prior to any reduction step. In 1989, we described a nonreductive mechanism for ferrioxamine B (FOB) uptake by S. cerevisiae (Lesuisse & Labbe, 1989). This siderophore can enter cells via an FOB receptor present at the cell surface.…”

Section: Introductionmentioning

confidence: 99%

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Siderophore uptake and use by the yeast Saccharomyces cerevisiae

et al. 2001

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The non-reductive uptake of several siderophores (ferrioxamine B, ferrichrome, triacetylfusarinine C and ferricrocin) by various strains of Saccharomyces cerevisiae was studied. Several aspects of siderophore transport were examined, including specificity of transport, regulation of transport and intracellular localization of the ferri-siderophores. Ferrioxamine B was taken up preferentially via the products of the SIT1 gene and triacetylfusarinine C by the TAF1 gene product, but the specificity was not absolute. Ferrichrome and ferricrocin uptake was not dependent on a single major facilitator superfamily (MFS) gene product. The apparent specificity of transport was strongly dependent on the genetic background of the cells. Non-reductive uptake of siderophores was induced under more stringent conditions (of iron deprivation) than was the reductive uptake of ferric citrate. Regulation of transport depended on the transcriptional factors Aft1 and Tup1/Ssn6. Cells disrupted for the TUP1 or SSN6 genes were constitutively derepressed for the uptake of ferrichrome, ferricrocin or ferrioxamine B, but not for the uptake of triacetylfusarinine C. Cells bearing the AFT1 up mutation accumulated large amounts of ferric siderophores. Intracellular decomplexation of the siderophores occurred when transcription of the AFT1 up gene was repressed. Ferrioxamine B and ferrichrome seemed to accumulate in an endosomal compartment, as shown by biochemical studies and by confocal microscopy study of cells loaded with a fluorescent derivative of ferrichrome. Endocytosis was, however, not involved in the non-reductive uptake of siderophores.

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Siderophore uptake and use by the yeast Saccharomyces cerevisiae

et al. 2001

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“…In this way, S. cerevisiae can transport siderophores excreted by other microorganisms, although it does not produce any itself. Non-reductive siderophore transport systems in this yeast have been described for ferrioxamine B (mesylate form) (FOB) (Lesuisse and Labbe, 1989;Lesuisse et al, 1998), ferrichrome (FCH) (Yun et al, 2000), triacetylfusarinine C (TAF) (Heymann et al, 1999), enterobactin (ENB) (Heymann et al, 2000a) and ferricrocin (FC) (Heymann et al, 2000b;Lesuisse et al, 2001). The gene products involved (Sit1p, Arn1p, Taf1p and Enb1p) belong to the major facilitator superfamily (MFS).…”

Section: Introductionmentioning

confidence: 99%

“…At low extracellular concentration (around 1 mM), uptake of the siderophore is essentially nonreductive. When the siderophore concentration is raised, the reductive mechanism becomes quantitatively more important as the non-reductive highaffinity system approaches saturation (Lesuisse and Labbe, 1989). The genes involved in both reductive and non-reductive uptake of iron by S. cerevisiae are transcriptionally regulated via the activator Aft1 (Yamaguchi-Iwai et al, 1995;Yun et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Siderophore uptake by Candida albicans: effect of serum treatment and comparison with Saccharomyces cerevisiae

Lesuisse

Knight

Camadro

et al. 2002

Yeast

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Iron uptake systems often function as virulence factors in pathogenic organisms. Candida albicans is a fungal pathogen that infects immunocompromised hosts, such as AIDS patients or granulocytopenic bone marrow transplant recipients. Here we show that iron uptake from siderophores occurs in C. albicans and is mediated by one or more highaffinity transport systems. Iron carried on ferrioxamine B, triacethyl-fusarinine, ferrichrome, or ferricrocin was actively taken up via a high-affinity mechanism. The kinetic parameters of uptake were similar to those found in S. cerevisiae. Furthermore, for ferrichrome and ferrioxamine B, cellular uptake of fluorescent analogues was observed. In C. albicans, iron uptake from siderophores was regulated by iron availability, with iron deprivation inducing uptake. Serum exposure, which induces a morphogenic shift from yeast to filamentous forms known to be required for virulence, also resulted in induction of iron transport from ferrichrome-type siderophores. In a tup1/tup1 strain which grows constitutively in the filamentous form, iron transport was derepressed for all siderophores tested. The genes mediating uptake and utilization of iron from siderophores in C. albicans have not been identified; however, the transcript abundance for CaSIT1 was regulated in a manner consistent with the pattern of iron uptake from ferrichrome-type siderophores. Furthermore, CaSIT1 overexpression in S. cerevisiae resulted in inhibited siderophore iron uptake, suggesting that the expressed protein may interact with proteins of S. cerevisiae involved in iron uptake from siderophores. In summary, iron uptake from ferrichrome-type siderophores was induced in filamentous C. albicans, and a potential role of this iron acquisition system in pathogenicity should be considered.

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The AFT1 Transcriptional Factor is Differentially Required for Expression of High‐Affinity Iron Uptake Genes in Saccharomyces cerevisiae

Casas

Aldea

Espinet

et al. 1997

Yeast

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High-affinity iron uptake in Saccharomyces cerevisiae involves the extracytoplasmic reduction of ferric ions by FRE1 and FRE2 reductases. Ferrous ions are then transported across the plasma membrane through the FET3 oxidase-FTR1 permease complex. Expression of the high-affinity iron uptake genes is induced upon iron deprivation. We demonstrate that AFT1 is differentially involved in such regulation. Aft1 protein is required for maintaining detectable non-induced levels of FET3 expression and for induction of FRE2 in iron starvation conditions. On the contrary, FRE1 mRNA induction is normal in the absence of Aft1, although the existence of AFT1 point mutations causing constitutive expression of FRE1 (Yamaguchi-Iwai et al., EMBO J. 14: [1231][1232][1233][1234][1235][1236][1237][1238][1239] 1995) indicates that Aft1 may also participate in FRE1 expression in a dispensable way. The alterations in the basal levels of expression of the high-affinity iron uptake genes may explain why the AFT1 mutant is unable to grow on respirable carbon sources. Overexpression of AFT1 leads to growth arrest at the G 1 stage of the cell cycle. Aft1 is a transcriptional activator that would be part of the different transcriptional complexes interacting with the promoter of the high-affinity iron uptake genes. Aft1 displays phosphorylation modifications depending on the growth stage of the cells, and it might link induction of genes for iron uptake to other metabolically dominant requirements for cell growth.

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Reductive and Non-reductive Mechanisms of Iron Assimilation by the Yeast Saccharomyces cerevisiae

Cited by 92 publications

References 14 publications

Siderophore uptake and use by the yeast Saccharomyces cerevisiae

Siderophore uptake and use by the yeast Saccharomyces cerevisiae

Siderophore uptake by Candida albicans: effect of serum treatment and comparison with Saccharomyces cerevisiae

The AFT1 Transcriptional Factor is Differentially Required for Expression of High‐Affinity Iron Uptake Genes in Saccharomyces cerevisiae

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