Reductive dechlorination of tetrachloroethene in marine sediments: Biodiversity and dehalorespiring capabilities of the indigenous microbes

Matturro, Bruna; Presta, E.; Rossetti, Simona

doi:10.1016/j.scitotenv.2015.12.098

Cited by 30 publications

(16 citation statements)

References 57 publications

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“…Engineered bioremediation and natural attenuation (NA) processes permit CAH removal from contaminated groundwater directly in situ, which usually results in an environmentally friendly and cost-effective treatment with respect to conventional technologies such as pump-and-treat [3][4][5]. The engineered bioremediation consists of stimulating the microbial activity that is naturally present in the soil/groundwater matrix to remove pollutants through their metabolic activity [6]. The highly chlorinated CAHs can be removed by a reductive dechlorination (RD) reaction through sequential steps in which the chlorinated molecule loses a chlorine atom at each step until the complete dechlorination of the molecule [7]: For this reaction, the microorganisms usually need an electron donor such as hydrogen, which can be produced by fermenting organic substrates that provide for the slow release of hydrogen [8].…”

Section: Introductionmentioning

confidence: 99%

Reductive/Oxidative Sequential Bioelectrochemical Process for Perchloroethylene Removal

Zeppilli

Dell’Armi

Cristiani

et al. 2019

Water

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An innovative bioelectrochemical reductive/oxidative sequential process was developed and tested on a laboratory scale to obtain the complete mineralization of perchloroethylene (PCE) in a synthetic medium. The sequential bioelectrochemical process consisted of two separate tubular bioelectrochemical reactors that adopted a novel reactor configuration, avoiding the use of an ion exchange membrane to separate the anodic and cathodic chamber and reducing the cost of the reactor. In the reductive reactor, a dechlorinating mixed inoculum received reducing power to perform the reductive dechlorination of perchloroethylene (PCE) through a cathode chamber, while the less chlorinated daughter products were removed in the oxidative reactor, which supported an aerobic dechlorinating culture through in situ electrochemical oxygen evolution. Preliminary fluid dynamics and electrochemical tests were performed to characterize both the reductive and oxidative reactors, which were electrically independent of each other, with each having its own counterelectrode. The first continuous-flow potentiostatic run with the reductive reactor (polarized at −450 mV vs SHE) resulted in obtaining 100% ± 1% removal efficiency of the influent PCE, while the oxidative reactor (polarized at +1.4 V vs SHE) oxidized the vinyl chloride and ethylene from the reductive reactor, with removal efficiencies of 100% ± 2% and 92% ± 1%, respectively.

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Section: Introductionmentioning

confidence: 99%

Reductive/Oxidative Sequential Bioelectrochemical Process for Perchloroethylene Removal

Zeppilli

Dell’Armi

Cristiani

et al. 2019

Water

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“…For example, polycyclic aromatic hydrocarbons (PAHs) are widespread pollutants of sediments ( Patel et al, 2013 ; Johnston and Leff, 2015 ; Louvado et al, 2015 ; Revathy et al, 2015 ; Waigi et al, 2015 ; Xia et al, 2015 ) and while anaerobic transformations of some PAH are known, aerobic processes tend to be more effective ( Doyle et al, 2008 ; Fernandez-Luqueno et al, 2011 ). Conversely, halogenated organic compounds such as polychlorinated biphenyls (PCBs) and trichloroethylene are relatively recalcitrant to degradation by aerobic processes, but have significant potential for transformation by anaerobes (i.e., those of Chloroflexi class Dehalococcoidia) that dehalogenate these compounds as part of a respiratory process ( Zanaroli et al, 2015 ; Matturro et al, 2016 ). Thus, an understanding about the impacts of pollutants upon sediment microbial communities should include consideration of effects on groups important in natural transformations, as well as those that may be active in biodegradation processes.…”

Section: Introductionmentioning

confidence: 99%

Microbial Communities in Sediments of Lagos Lagoon, Nigeria: Elucidation of Community Structure and Potential Impacts of Contamination by Municipal and Industrial Wastes

et al. 2016

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Estuarine sediments are significant repositories of anthropogenic contaminants, and thus knowledge of the impacts of pollution upon microbial communities in these environments is important to understand potential effects on estuaries as a whole. The Lagos lagoon (Nigeria) is one of Africa’s largest estuarine ecosystems, and is impacted by hydrocarbon pollutants and other industrial and municipal wastes. The goal of this study was to elucidate microbial community structure in Lagos lagoon sediments to identify groups that may be adversely affected by pollution, and those that may serve as degraders of environmental contaminants, especially polycyclic aromatic hydrocarbons (PAHs). Sediment samples were collected from sites that ranged in types and levels of anthropogenic impacts. The sediments were characterized for a range of physicochemical properties, and microbial community structure was determined by Illumina sequencing of the 16S rRNA genes. Microbial diversity (species richness and evenness) in the Apapa and Eledu sediments was reduced compared to that of the Ofin site, and communities of both of the former two were dominated by a single operational taxonomic unit (OTU) assigned to the family Helicobacteraceae (Epsilonproteobacteria). In the Ofin community, Epsilonproteobacteria were minor constituents, while the major groups were Cyanobacteria, Bacteroidetes, and Firmicutes, which were all minor in the Apapa and Eledu sediments. Sediment oxygen demand (SOD), a broad indicator of contamination, was identified by multivariate analyses as strongly correlated with variation in alpha diversity. Environmental variables that explained beta diversity patterns included SOD, as well as levels of naphthalene, acenaphthylene, cobalt, cadmium, total organic matter, or nitrate. Of 582 OTU identified, abundance of 167 was significantly correlated (false discovery rate q≤ 0.05) to environmental variables. The largest group of OTU correlated with PAH levels were PAH/hydrocarbon-degrading genera of the Oceanospirillales order (Gammaproteobacteria), which were most abundant in the hydrocarbon-contaminated Apapa sediment. Similar Oceanospirillales taxa are responsive to marine oil spills and thus may present a unifying theme in marine microbiology as bacteria adapted for degradation of high hydrocarbon loads, and may represent a potential means for intrinsic remediation in the case of the Lagos lagoon sediments.

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“…More recently the RD capability of some strains of D. mccartyi (CBDB1, JN, CG3, CG4, CG5) carrying specialized PCB-dechlorinase genes ( pcbA1 , pcbA4 , pcbA5 ) and other Chloroflexi members, similar but distantly related to D. mccartyi such as Dehalobium chlorocoercia DF-1, strain o-17 , phylotype SF-1 and phylotype VL-CHL1 was shown ( Cutter et al, 2001 ; Bedard, 2008 ; Zanaroli et al, 2012a ; Wang and He, 2013 ; Wang et al, 2014 , 2015 ; Matturro et al, 2016a , b ).…”

Section: Introductionmentioning

confidence: 99%

Microbiome Dynamics of a Polychlorobiphenyl (PCB) Historically Contaminated Marine Sediment under Conditions Promoting Reductive Dechlorination

2016

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The toxicity of polychlorinated biphenyls (PCB) can be efficiently reduced in contaminated marine sediments through the reductive dechlorination (RD) process lead by anaerobic organohalide bacteria. Although the process has been extensively investigated on PCB-spiked sediments, the knowledge on the identity and metabolic potential of PCB-dechlorinating microorganisms in real contaminated matrix is still limited. Aim of this study was to explore the composition and the dynamics of the microbial communities of the marine sediment collected from one of the largest Sites of National Interest (SIN) in Italy (Mar Piccolo, Taranto) under conditions promoting the PCBs RD. A long-term microcosm study revealed that autochthonous bacteria were able to sustain the PCB dechlorination at a high extent and the successive addition of an external fermentable organic substrate (lactate) caused the further depletion of the high-chlorinated PCBs (up to 70%). Next Generation Sequencing was used to describe the core microbiome of the marine sediment and to follow the changes caused by the treatments. OTUs affiliated to sulfur-oxidizing ε-proteobacteria, Sulfurovum, and Sulfurimonas, were predominant in the original sediment and increased up to 60% of total OTUs after lactate addition. Other OTUs detected in the sediment were affiliated to sulfate reducing (δ-proteobacteria) and to organohalide respiring bacteria within Chloroflexi phylum mainly belonging to Dehalococcoidia class. Among others, Dehalococcoides mccartyi was enriched during the treatments even though the screening of the specific reductive dehalogenase genes revealed the occurrence of undescribed strains, which deserve further investigations. Overall, this study highlighted the potential of members of Dehalococcoidia class in reducing the contamination level of the marine sediment from Mar Piccolo with relevant implications on the selection of sustainable bioremediation strategies to clean-up the site.

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Reductive dechlorination of tetrachloroethene in marine sediments: Biodiversity and dehalorespiring capabilities of the indigenous microbes

Cited by 30 publications

References 57 publications

Reductive/Oxidative Sequential Bioelectrochemical Process for Perchloroethylene Removal

Reductive/Oxidative Sequential Bioelectrochemical Process for Perchloroethylene Removal

Microbial Communities in Sediments of Lagos Lagoon, Nigeria: Elucidation of Community Structure and Potential Impacts of Contamination by Municipal and Industrial Wastes

Microbiome Dynamics of a Polychlorobiphenyl (PCB) Historically Contaminated Marine Sediment under Conditions Promoting Reductive Dechlorination

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