Reductive dehalogenation of chlorinated dioxins by an anaerobic bacterium

Bunge, Michael; Adrian, Lorenz; Kraus, A.; Opel, Matthias; Lorenz, W.; Andreesen, Jan R.; Görisch, Helmut; Lechner, Ute

doi:10.1038/nature01237

Cited by 316 publications

(252 citation statements)

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“…The D. mccartyi strain CBDB1 is adapted to organohalide respiration with chlorinated aromatic compounds such as different chlorinated benzenes, phenols, biphenyls and even chlorinated dibenzo-p-dioxins [32][33][34][35]. Figure 1 shows three selected chloroaromatic compounds and the dechlorination route observed with strain CBDB1.…”

Section: Introductionmentioning

confidence: 99%

“…Figure 1 shows three selected chloroaromatic compounds and the dechlorination route observed with strain CBDB1. With regard to the dechlorination of polychlorinated dibenzo-p-dioxins, strain CBDB1 can even dechlorinate the most toxic congeners 1,2,3,7,8-penta-and 2,3,7,8-tetrachlorodibenzo-p-dioxin to 2,7-or 2,8-dichlorodibenzo-p-dioxin (DCDD), resulting in a strong reduction in the toxicity [33]. The transcriptional response of all 32 rdhA genes of strain CBDB1 during dechlorination of 1,2,3-and 1,2,4-trichlorobenzenes (TCBs) was investigated using a combined reverse transcription (RT)-PCR/terminal restriction fragment length polymorphism analysis (t-RFLP) approach [36].…”

Section: Introductionmentioning

confidence: 99%

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Regulation of reductive dehalogenase gene transcription in Dehalococcoides mccartyi

Wagner

Segler

Kleinsteuber

et al. 2013

Phil. Trans. R. Soc. B

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The remarkable capacity of the genus Dehalococcoides to dechlorinate a multitude of different chlorinated organic compounds reflects the number and diversity of genes in the genomes of Dehalococcoides species encoding reductive dehalogenase homologues ( rdh ). Most of these genes are located in the vicinity of genes encoding multiple antibiotic resistance regulator (MarR)-type or two-component system regulators. Here, the transcriptional response of rdhA genes (coding for the catalytic subunit) to 2,3- and 1,3-dichlorodibenzo- p -dioxin (DCDD) was studied in Dehalococcoides mccartyi strain CBDB1. Almost all rdhA genes were transcribed in the presence of 2,3-DCDD, albeit at different levels as shown for the transcripts of cbrA , cbdbA1453, cbdbA1624 and cbdbA1588. By contrast, 1,3-DCDD did not induce rdhA transcription. The putative MarR CbdbA1625 was heterologously produced and its ability to bind in vitro to the overlapping promoter regions of the genes cbdbA1624 and cbdbA1625 was demonstrated. To analyse regulation in vivo , single-copy transcriptional promoter– lacZ fusions of different rdhA genes and of cbdbA1625 were constructed and introduced into the heterologous host Escherichia coli , and expression levels of the fusions were measured. The cbdbA1625 gene was cloned into a vector allowing a regulation of expression by arabinose and it was transformed into the strains containing the rdh -promoter– lacZ fusion derivatives. CbdbA1625 was shown to downregulate transcription from its own promoter resulting in a 40–50% reduction in the β-galactosidase activity, giving the first hint that it acts as a repressor.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Regulation of reductive dehalogenase gene transcription in Dehalococcoides mccartyi

Wagner

Segler

Kleinsteuber

et al. 2013

Phil. Trans. R. Soc. B

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“…1) Several bacterial groups, e.g., a sulfur-reducing bacterial group such as Desulfitobacterium sp., 2) and phylum Chloroflexi, such as Dehalococcoides sp., 3) were reported to have anaerobic dehalorespiration, which reductively dechlorinated tetrachloroethene, 2) chlorobenzenes, 4) or chlorophenols or dioxins. 5) Here we represent our results on the enrichment and isolation of anaerobic microorganisms, which could degrade various kinds of POPs under anaerobic conditions. 6,7) Materials and Methods 8) The medium used in this study was designated to enrich microorganisms which use halogenated compounds as electron acceptors.…”

Section: Introductionmentioning

confidence: 99%

Enrichment and isolation of anaerobic microorganisms concerned with reductive degradation of hexachlorobenzene from soils

Watanabe

Yoshikawa

2008

J. Pestic. Sci.

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Enrichment culture media, which reduced the recovery ratios of residual hexachlorobenzene (200 ppm) to several % within 2 weeks, were prepared after 4 transfers of paddy field soil and PCB-treated flooded soil. Eleven morphologically identical anaerobic microorganisms, which had a reduced residual HCB recovery ratio, were isolated using aerobic agar plates. The isolated microorganisms remarkably reduced the recovery ratio of residual heptachlor but their reduction of endrin, aldrin, and dieldrin was not so remarkable, and had novel morphological and physiological characters, which clearly distinguished these microorganisms from any of the known microorganisms. © Pesticide Science Society of Japan

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“…A distant relative of Dehalococcoides spp., "Dehalobium chlorocoercia" DF-1, dechlorinates HCB only to 1,3,5-TCB (48). Besides HCB, strain CBDB1 can also degrade polychlorinated dibenzodioxins, ethenes, phenols, and benzenes (1,3,10), whereas strain 195 can also use chlorinated ethenes and ethanes as electron acceptors (14,31). Enzymes catalyzing the reductive dechlorination of HCB have not yet been identified.…”

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Role of “Dehalococcoides” spp. in the Anaerobic Transformation of Hexachlorobenzene in European Rivers

Taş

Eekert

Wagner

et al. 2011

Appl Environ Microbiol

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The diffuse pollution by chlorinated organic compounds in river basins is a concern, due to their potential adverse effects on human health and the environment. Organohalides, like hexachlorobenzene (HCB), are recalcitrant to aerobic microbial degradation, and "Dehalococcoides" spp. are the only known microorganisms capable of anaerobic transformation of these compounds coupled to their growth. In this study, sediments from four European rivers were studied in order to determine their HCB dechlorination capacities and the role of Dehalococcoides spp. in this process. Only a weak correlation was observed between Dehalococcoides species abundance and HCB transformation rates from different locations. In one of these locations, in the Ebro River sediment, HCB dechlorination could be linked to Dehalococcoides species growth and activity by 16S rRNAbased molecular methods. Furthermore, HCB dechlorination activity in this sediment was found over the full range of ambient temperatures that this sediment can be exposed to during different seasons throughout the year. The sediment contained several reductive dehalogenase (rdh) genes, and analysis of their transcription revealed the dominance of cbrA, previously shown to encode a trichlorobenzene reductive dehalogenase. This study investigated the role of Dehalococcoides spp. in HCB dechlorination in river sediments and evaluated if the current knowledge of rdh genes could be used to assess HCB bioremediation potential.Production of hexachlorobenzene (HCB)-containing pesticides is banned in most of the world due to HCB's toxic and carcinogenic nature. However, production and emission of the compound still occurs as an intermediate in chemical processes and from natural sources, such as volcanoes (5, 17). In aquatic environments, HCB is mainly deposited in the sediment due to its hydrophobicity. Bacterial reductive dechlorination plays an important role in the degradation of chlorinated aromatic contaminants like HCB in these anaerobic environments (15,19). Under anaerobic conditions, HCB is used by some bacteria in their energy metabolism by coupling reductive dehalogenation to electron transport phosphorylation (36). This is the only known pathway, so far, for the microbial transformation of HCB that is linked to microbial growth. Reductive dechlorination of HCB and its lesser chlorinated derivatives was previously reported in several river sediments (8,11,18,19). Nevertheless, in none of these studies were the microorganisms responsible for the process characterized.Three strains of bacteria capable of degrading HCB via reductive dechlorination have been isolated so far. "Dehalococcoides" sp. strain CBDB1 and "Dehalococcoides ethenogenes" strain 195 dechlorinate HCB to 1,3-dichlorobenzene (DCB), 1,4-DCB, and 1,3,5-trichlorobenzene (TCB) (3,14). Additionally, production of 1,2-DCB was also observed with strain 195. A distant relative of Dehalococcoides spp., "Dehalobium chlorocoercia" DF-1, dechlorinates HCB only to 1,3,5-TCB (48). Besides HCB, strain CBDB1 can also degr...

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Reductive dehalogenation of chlorinated dioxins by an anaerobic bacterium

Cited by 316 publications

References 25 publications

Regulation of reductive dehalogenase gene transcription in Dehalococcoides mccartyi

Regulation of reductive dehalogenase gene transcription in Dehalococcoides mccartyi

Enrichment and isolation of anaerobic microorganisms concerned with reductive degradation of hexachlorobenzene from soils

Role of “Dehalococcoides” spp. in the Anaerobic Transformation of Hexachlorobenzene in European Rivers

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