Recent claims for continent wide disappearance of megafauna at 46.5 thousand calendar years ago (ka) in Australia have been used to support a ''blitzkrieg'' model, which explains extinctions as the result of rapid overkill by human colonizers. A number of key sites with megafauna remains that significantly postdate 46.5 ka have been excluded from consideration because of questions regarding their stratigraphic integrity. Of these sites, Cuddie Springs is the only locality in Australia where megafauna and cultural remains are found together in sequential stratigraphic horizons, dated from 36 -30 ka. Verifying the stratigraphic associations found here would effectively refute the rapid-overkill model and necessitate reconsideration of the regional impacts of global climatic change on megafauna and humans in the lead up to the last glacial maximum. Here, we present geochemical evidence that demonstrates the coexistence of humans and now-extinct megafaunal species on the Australian continent for a minimum of 15 ka.archeology ͉ extinction ͉ geochemistry ͉ rare earth element ͉ climate change L ate Quaternary extinctions of megafauna have been documented on all continents except Antarctica, and in North America and Australia, these extinctions have broadly coincided with human colonization (1, 2). In Australia, conservative estimates place human arrival on this continent at Ϸ43-45 thousand calendar years ago (ka), although some researchers argue for colonization up to 60 ka (3). Intense debate surrounds the timing and causes of megafaunal extinctions in Australia, especially in resolving the relative roles of humans and climate (1-6). The following three explanatory hypotheses ( Fig. 1) dominate the discussion.1. Human overkill, or ''blitzkrieg,'' in which megafauna went extinct within 1,000 years of human arrival, with megafauna disappearing by 46.5 ka. 2. Habitat modification by humans through firing the landscape and associated hunting, with extinctions complete by 46.5 ka. 3. A paleoecological explanation in which climate change was the driving force in megafauna demise as factors such as increasing aridity, habitat reconfiguration, competitive exclusion, and possibly the added pressures of a new predator combined to suppress and ultimately drive to extinction a suite of Australian fauna.Developing extinction chronologies has generally relied on the dating of sediments associated with megafaunal remains (2). Direct dating of skeletal material from Australian late Pleistocene contexts has been problematic, and few studies have produced unequivocal results (7,8). The analysis of rare earth element (REE) contents in bone has emerged recently as a valuable test for stratigraphic associations (9-13). In buried bone, REEs are adsorbed rapidly from pore waters onto bone crystal surfaces and then locked in the bone crystal lattice during recrystallization (9). The relative abundances of REEs sequestered into bone are controlled by the immediate pore water chemistry. Therefore, bones in successive depositional units frequen...