Reevaluating Genetic Diversity and Structure of Helianthus verticillatus (Asteraceae) after the Discovery of New Populations

Moore-Pollard, Erika R.; Siniscalchi, Carolina M.; Mandel, Jennifer R.

doi:10.2179/0008-7475.86.2.196

Cited by 2 publications

(2 citation statements)

References 55 publications

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“…Helianthus verticillatus Small, commonly known as the whorled sunflower, stands out as a perennial species within the Helianthus genus, characterized by its unique features. Designated as federally endangered in 2014 [ 4 ], H. verticillatus is restricted to a few locations in southeastern United States, underscoring its rarity [ 5 , 6 , 7 ]. Its appealing inflorescences, adorned with multiple showy yellow ray flowers, position it as a potential ornamental plant and a crucial resource for numerous insect pollinators [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Regeneration from Leaf Explants of Helianthus verticillatus, a Critically Endangered Sunflower

Nowakowska,

Pavlovic,

Nowicki

et al. 2024

Plants

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Helianthus verticillatus (Asteraceae), a whorled sunflower, is a perennial species restricted to a few locations in the southeastern United States and is now considered endangered. Therefore, restoring and protecting H. verticillatus as a species is a priority. This study introduces a highly efficient in vitro adventitious plant regeneration system from leaf explants, utilizing five diverse specimens of H. verticillatus, each representing distinct genotypes with phenotypic variations in leaf and stem morphology. Key factors influencing in vitro morphogenesis, including genetic constitution, explant source, and plant growth regulators (PGRs), were identified. The study revealed a remarkably strong genotype-dependent impact on the regeneration efficiency of the investigated H. verticillatus genotypes, ranging from a lack of regeneration to highly effective regeneration. The selection of two genotypes with varying regeneration abilities provides valuable models for genetic analyses, offering insights into factors influencing the regeneration potential of this endangered species. Optimum adventitious shoot regeneration results were achieved using Murashige and Skoog basal media (MS) supplemented with 8.8 µM N6-benzyladenine (BA) and 1.08 µM α-naphthalene acetic acid (NAA). This combination yielded the highest adventitious shoot production. Subsequent successful rooting on ½ MS medium without PGRs further solidified the efficiency of the developed protocol. Regenerated plantlets, demonstrating robust shoots and roots, were successfully acclimatized to greenhouse conditions with a 95% survival rate. The protocol developed in this study is the first such report for this endangered species and is expected to contribute to future genetic manipulation and modification studies.

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Section: Introductionmentioning

confidence: 99%

In Vitro Regeneration from Leaf Explants of Helianthus verticillatus, a Critically Endangered Sunflower

Nowakowska,

Pavlovic,

Nowicki

et al. 2024

Plants

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show abstract

“…There are more than 50 wild Helianthus species (Schilling 2006;Seiler et al 2018) of which, H. verticillatus Small, the whorled sunflower, is a perennial species and was listed as critically endangered in 2014 (US Fish and Wildlife Service 2014). The whorled sunflower has very limited distribution in the southeastern United States and is restricted to a few locations in Tennessee, Alabama, Virginia, Georgia, and Mississippi (Mandel 2010;Moore et al 2022). A method to conserve rare, self-incompatible plants is to establish gardens or plots with a wide assortment of compatible genotypes to facilitate seed production.…”

mentioning

confidence: 99%

‘Denita’s Autumn Sunshine’: A Cultivar of the Endangered Sunflower Helianthus verticillatus

Trigiano,

Boggess,

Odoi

et al. 2024

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Reevaluating Genetic Diversity and Structure of Helianthus verticillatus (Asteraceae) after the Discovery of New Populations

Cited by 2 publications

References 55 publications

In Vitro Regeneration from Leaf Explants of Helianthus verticillatus, a Critically Endangered Sunflower

In Vitro Regeneration from Leaf Explants of Helianthus verticillatus, a Critically Endangered Sunflower

‘Denita’s Autumn Sunshine’: A Cultivar of the Endangered Sunflower Helianthus verticillatus

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