2004
DOI: 10.1016/j.bbagen.2004.08.006
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Reevaluating the role of 1,10-phenanthroline in oxidative reactions involving ferrous ions and DNA damage

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Cited by 101 publications
(56 citation statements)
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“…Hydroxyl radical-scavenging assay was carried out using the method described by de Avellar et al [16] with some modifications. Both 1,10-phenanthroline (0.75 mM) and FeSO 4 (0.75 mM) were dissolved in phosphate buffer (pH 7.4) and mixed thoroughly.…”
Section: Evaluation Of Hydroxyl Radical-scavenging Activitymentioning
confidence: 99%
“…Hydroxyl radical-scavenging assay was carried out using the method described by de Avellar et al [16] with some modifications. Both 1,10-phenanthroline (0.75 mM) and FeSO 4 (0.75 mM) were dissolved in phosphate buffer (pH 7.4) and mixed thoroughly.…”
Section: Evaluation Of Hydroxyl Radical-scavenging Activitymentioning
confidence: 99%
“…The total carbohydrate content in AAP was 42.5%, consisting of rhamnose, xylose, glucose, uronic acids, and sulfate as main composition besides smaller amounts of mannose, galactose, and arabinose, as reported in our previous research [10]. The total phenolic compounds in HPC and PPC was 102 and 53.5 mg/g, respectively, expressed as gallic acid equivalent.…”
Section: Identification Of Marker Compoundsmentioning
confidence: 76%
“…The polysaccharides of A.auricula (AAP), polyphenolic components of hawthorn (HPC) and flavanoids components of Pueraria radix (PPC) were extracted and lyophilized according to our previous reported procedures [9,10]. Through our preliminary trials, the optimal formulation of weight ratio of three individual components in AHP was set as 5 (AAP): 3 (HPC): 2 (PPC).…”
Section: Preparation Of Functional Formula Dietmentioning
confidence: 99%
“…These results show that the SSPH fractions are good scavengers of hydroxyl free radicals when compared to the values reported by Mau, Lin, and Song (2002) for BHA (23%) and α-tocopherol (34%) at concentrations of 3.6 and 8.6 mg/mL, respectively. One of the most commonly used iron chelators for the prevention of ironmediated OH formation in vitro, and also as a tool to indicate the participation of iron in oxidative stress processes is 1,10-phenathroline (de Avellar et al, 2004). In the presence of other competitive chelators, the [Fe(phen) 3 ] 2+ complex is disrupted, leading to the decrease in the dark orange color of the complex.…”
Section: Hydroxyl Rsamentioning
confidence: 99%