Reevaluation of glypican-3 as a prognostic marker in HCC using X-tile software

Pan, Chen‐Wei; Wang, Xiaodong; Chen, Weilai; Tao, Chonglin; Xu, Xiaorong; Chen, Yongping; Zhu, Lihe; Zhou, Lingli; Pan, Zhenzhen

doi:10.1007/s12032-014-0359-z

Cited by 23 publications

(32 citation statements)

References 19 publications

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“…In this study, we demonstrate that regardless of the expression pattern, the presence of GPC3 expression was associated with more frequent AFP elevation, microvascular invasion, and hepatic causes of death, suggestive of a more aggressive behavior in GPC3-positive HCCs. These results are in line with previous reports that have associated GPC3 positivity with vascular invasion and AFP elevation (12,(15)(16)(17). When the pattern of GPC3 staining was taken into account, we found that the cases with diffuse GPC3 expression were associated with younger age (although marginally significant), higher serum AFP levels, and more frequent microvascular invasion compared with HCCs with focal GPC3 expression.…”

Section: Discussionsupporting

confidence: 92%

“…A recent review (4) attributed the large range of survival to differences in HCC staging. Our own case series included patients who underwent various anti-cancer treatments such as TACE and RFA, while some prognostic studies enrolled patients with no prior treatment (15,18). However, we did not find Determined by log-rank test for comparison of overall survival and disease-free survival between GPC3-positive and GPC3-negative group.…”

Section: Discussionmentioning

confidence: 86%

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Expression profile and prognostic value of glypican‐3 in post‐operative South Korean hepatocellular carcinoma patients

Jeon

Kim

Jang

et al. 2016

APMIS

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Hepatocellular carcinoma (HCC) patients commonly experience poor overall survival (OS) and disease-free survival (DFS) after curative surgical resection. Glypican-3 (GPC3) has been suggested as a prognostic biomarker for post-operative survival. However, few to none of these studies have included South Korean patients. This study aimed to determine GPC3 expression rate, clinical correlation, and post-operative prognostic value in South Korean HCC patients who underwent curative surgical resection. Surgically resected tissues from 185 HCC patients were collected and assembled into tissue microarrays (TMAs), which were stained for GPC3 by immunohistochemistry. GPC3 expression rates were correlated with clinicopathological information, and survival analyses were performed to assess the prognostic value of GPC3. GPC3 expression was present in 153 patients (82.7%). GPC3-positive patients were younger with higher frequencies of microvascular invasion and higher AFP levels than GPC3-negative patients. There was no significant difference in survival between GPC3-negative and GPC3-positive patients. Based on multivariate analysis, GPC3 expression was not a prognostic marker for post-operative survival. In South Korean HCC patients, GPC3 expression was more frequent in HCCs with aggressive features, but it was not an independent prognostic biomarker.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 86%

Expression profile and prognostic value of glypican‐3 in post‐operative South Korean hepatocellular carcinoma patients

Jeon

Kim

Jang

et al. 2016

APMIS

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“…HRs and its 95% CIs of OS and DFS were extracted from all included articles or calculated indirectly by the methods described above. Initial treatment for patients with HCC with surgical resection was performed in 11 studies [ 18 – 22 , 26 – 29 , 31 , 35 ] and liver transplantation (LT) in 1 article. [ 30 ] The scope of sample size in all studies varies from 31 to 362.…”

Section: Resultsmentioning

confidence: 99%

Overexpression of glypican-3 is a predictor of poor prognosis in hepatocellular carcinoma

Zhang

et al. 2018

Medicine

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Background:Glypican-3 (GPC3) has been widely recognized in the progression of liver tumors for several years. The relationship between overexpression of GPC3 and the poorer prognosis of patients with hepatocellular carcinoma (HCC) was performed by 2 meta-analyses. However, there were also some latest literatures that indicated different conclusions distinctly. It is necessary for us to carry out a meta-analysis by adding the latest data from current studies to explore the correlation between GPC3 and prognostic value in HCC.Methods:We conducted a meta-analysis including a total of 14 studies to assess the potential prognostic significance of GPC3 expression for overall survival (OS) and disease-free survival (DFS). The expression of GPC3 was assessed by immunohistochemistry.Results:Fourteen studies with 2364 patients were incorporated in the meta-analysis. The combined hazard ratios (HRs) revealed that the overexpression of GPC3 could forecast a poor OS [n = 2233 in 12 studies, HR = 1.40, 95% confidence interval (95% CI): 1.07–1.85, Z = 2.42, P = .02] and DFS (n = 1308 in 10 studies, HR = 1.61, 95% CI: 1.13–2.30, Z = 2.63, P = .008) in HCC patients. Subgroup treated by hepatectomy indicated that the pooled HR of OS was 1.43 (95% CI: 1.01–2.01, P = .04) and the combined HR of DFS was 1.59 (95% CI: 1.09–2.31, P = .02). The pooled odds ratios (ORs) showed that high GPC3 expression was also extensively associated with worse tumor differentiation, later tumor stage, presence of vascular invasion, and hepatitis B virus (HBV) infection. Subgroup analyses for GPC3 on HCC OS based on the studies categorized by regions, follow-up period, and sample size were also conducted.Conclusion:The meta-analysis indicated that overexpression of GPC3 was significantly associated with poor prognosis in patients with HCC.

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“…An optimal cutoff value for the IHC score that separates patients into low and high CNPY2 isoform2 expression groups with respect to OS was determined using X-tile software (version 3.6.1; Yale University, New Haven, CT, USA) at the largest chi-square value in the training cohort, as described previously[ 25 , 26 ]. Categorical variables are presented as percentages, and they were compared using the chi-square test or Fisher's exact test.…”

Section: Methodsmentioning

confidence: 99%

Expression of a novel CNPY2 isoform in colorectal cancer and its association with oncologic prognosis

Peng

Ou²,

Guo³

et al. 2017

Aging

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Colorectal cancer (CRC) is a leading cause of cancer-related mortality. Recently, we identified a novel biomarker, canopy fibroblast growth factor signaling regulator 2 (CNPY2) isoform2, and subsequently investigated its expression and prognostic value in CRC patients. We initially generated CNPY2 isoform2 monoclonal antibodies and examined CNPY2 isoform2 expression in CRC cell lines and tissues using quantitative real-time polymerase chain reaction, western blot and immunohistochemistry analyses. We found that CNPY2 isoform2 expression significantly increased in tumor cell lines and tissues compared with that in normal colon epithelial cells and tumor-adjacent normal tissues. Survival analysis indicated that patients with low CNPY2 isoform2 expression had poorer 5-year overall survival (OS) in both the training cohort (41.7% vs. 77.7%, P = 0.007) and validation cohort (47.1% vs. 78.8%, P = 0.002). In multivariable analysis, CNPY2 isoform2 was identified as a predictor of 5-year OS in both the training cohort [hazard ratio (HR) = 5.001; 95% confidence interval (CI) 2.156–11.598, P < 0.001) and validation cohort (HR= 2.443; 95% CI 1.197- 4.983, P = 0.014). In conclusion, CNPY2 isoform2 represents as a novel and valuable prognostic indicator for CRC patients, while the oncologic function of CNPY2 requires further study.

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Reevaluation of glypican-3 as a prognostic marker in HCC using X-tile software

Cited by 23 publications

References 19 publications

Expression profile and prognostic value of glypican‐3 in post‐operative South Korean hepatocellular carcinoma patients

Expression profile and prognostic value of glypican‐3 in post‐operative South Korean hepatocellular carcinoma patients

Overexpression of glypican-3 is a predictor of poor prognosis in hepatocellular carcinoma

Expression of a novel CNPY2 isoform in colorectal cancer and its association with oncologic prognosis

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