Reevaluation of Gray and White Matter Injury after Spinal Cord Ischemia in Rabbits

Abstract

The results in the current study show that SCI induced white matter injury as well as gray matter injury in a rabbit model of SCI. The time course for 14 days after reperfusion may differ among the gray and white matter damages and hind-limb motor function in rabbits subjected to SCI.

Evaluation of the Neuroprotective Effect of Minocycline in a Rabbit Spinal Cord Ischemia Model

“…In the present study, a rabbit model of SCI was used due to the following reasons. First, in a rat SCI model, it has been reported that motor function gradually improved without no delayed deterioration, while hind-limb motor function deteriorated after SCI over 2 weeks in a rabbit model (21). Delayed development of paraplegia is well known in human, so that the time course after SCI in human may resemble to that in rabbit.…”

Effects of xenon on ischemic spinal cord injury in rabbits: a comparison with propofol

“…Finally, we cannot conclude about the neuroprotective effect of xenon on white matter injury because white matter injury may not appear within 48 h after reperfusion. Kurita et al 23 reported that white matter injury after spinal cord ischemia did not appear 24 h after reperfusion, but was apparent 4 days after reperfusion in rabbits. Longer recovery periods might be necessary for the assessments of white matter injury.…”

Co-application of ischemic preconditioning and postconditioning provides additive neuroprotection against spinal cord ischemia in rabbits

“…The vacuolation indicates a segmental swelling of myelinated axons, the formation of spaces between myelin sheaths and axolemma, and astrocyte swelling (Kurita et al, 2006). APP is transported by fast anterograde axonal transport; therefore, the accumulation of APP at the sites of injury, accompanied by morphologic evidence of axonal damage in the form of axonal swelling or bulbs, has been regarded as evidence of axonal injury (Kurita et al, 2006). Our data demonstrate that combination of IPC and postconditioning attenuated white matter injury as well as gray matter injury resulting from spinal cord ischemia.…”

“…The percent area of vacuolation of the three target areas was calculated and the data were then averaged (Kurita et al, 2006).…”

“…APP immunoreactivity was assessed in three areas of ventral, ventrolateral and lateral white matter with a dimension of 200 µm. Each area was divided into nine squares and a score of 0 (no APP accummulation) or 1 (APP accummulation in axonal swelling) was assigned to each square (Shi et al, 2007;Kurita et al, 2006). The total score of both sides in each animal was calculated (from 0 to 54).…”