Reevaluation of the Subunit Molecular Weights of Soybean 11S Globulin

A high-resolution carbon-13 nuclear magnetic resonance study of glycinin in deuterium oxide solutions

“…SDS-PAGE under reducing conditions yielded two major bands at 39 000 and 19 000 identified as the acidic and basic subunits of glycinin, respectively. The measured MW values generally agree with those reported in the literature (Ochiai-Yanagi et al, 1977;Moreira et al, 1979; Nielsen, 1985); these might, however, represent an overestimate, particularly with regard to the acidic subunits, since molecular weight estimates by gel filtration under denaturing conditions may not be meaningful (Ochiai-Yanagi et al, 1977), whereas those derived from the primary structure (Nielsen, 1985) yielded lower values. The reason for the overestimate may be related to the high percentage of acidic amino acid residues (Ochiai-Yanagi et al, 1977).…”

Polymerization of soybean 11S globulin due to reactions with peroxidizing linoleic acid.

“…Ochiai-Yanagi et a1. 22, also reported that the basic subunit had a higher lysine amount than the acidic subunit. The amino acid analysis of the crude 11S globulin used in the present experiments showed that the acidic and basic subunit fractions contained 4.4 g and 5.1 g lysine re sidues per 100 g protein, respectively (Table I).…”

Polymerization of Soybean 11S Globulin Due to Reactions with Peroxidizing Linoleic Acid

“…Ochiai-Yanagi et aI. 22) also reported that the basic subunit had a higher lysine amount than the acidic subunit. The amino acid analysis of the crude lIS globulin used in the present experiments showed that the acidic and basic subunit fractions contained 4.4 g and 5.1 g lysine residues per 100 g protein, respectively (Table 1).…”