Reexamining the Role of Pulmonary Lipids in the Pathogenesis of Pulmonary Fibrosis

O’Callaghan, Marissa; Tarling, Elizabeth J.; Bridges, James P.; Redente, Elizabeth F.; Byrne, Adam J.; Keane, Michael P.; McCarthy, Cormac

doi:10.1165/rcmb.2024-0124ps

Am J Respir Cell Mol Biol

2024

DOI: 10.1165/rcmb.2024-0124ps

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Reexamining the Role of Pulmonary Lipids in the Pathogenesis of Pulmonary Fibrosis

Marissa O’Callaghan,

Elizabeth J. Tarling,

James P. Bridges

et al.

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2024

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Cited by 1 publication

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Siglec-F Deficiency Prevents Fibrosis After Bleomycin-Induced Acute Lung Injury

Orlov,

Rangarajan,

Jaramillo

et al. 2024

Preprint

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Injury to the lungs causes acute inflammation that can lead to pathological lung fibrosis. Airspace macrophages (AMs) are critical for repair of injured tissue, but they can contribute fibrosis through mechanisms that are incompletely understood. Siglecs are expressed by immune cells. In mice, Siglec-F is chiefly expressed AMs where it is considered inflammosuppressive. We hypothesized that its deletion would worsen lung injury and fibrosis in response to intratracheal bleomycin challenge. We evaluated Siglec-F expression and function in mice challenged with bleomycin on days 7, 14, and 21 post-challenge (2.5 U/kg). AMs were the predominant inflammatory cells at all timepoints, and they included resident (RAM) and recruited (RecAM) subsets. Siglec-F deficiency prevented fibrosis than in Siglecf−/− mouse lungs, as evident from biochemical and histologic readouts. We performed RNAseq on pooled RAMs and RecAMs from wild type and Siglec-F deficient mice. Lung fibrosis 21 d after bleomycin challenge was associated with differentially expressed genes (DEGs) related to cholesterol synthesis and metabolism. In AMs from healthy lung lavage fluid and idiopathic pulmonary fibrosis patient tissues, the human paralogs Siglec-7 and Siglec-9 were expressed. Findings here identify novel mechanisms that control protective and detrimental functions of AMs after lung injury.

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Siglec-F Deficiency Prevents Fibrosis After Bleomycin-Induced Acute Lung Injury

Orlov,

Rangarajan,

Jaramillo

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

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Reexamining the Role of Pulmonary Lipids in the Pathogenesis of Pulmonary Fibrosis

Cited by 1 publication

References 161 publications

Siglec-F Deficiency Prevents Fibrosis After Bleomycin-Induced Acute Lung Injury

Siglec-F Deficiency Prevents Fibrosis After Bleomycin-Induced Acute Lung Injury

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